FastQCFastQC Report
Fri 10 Feb 2017
ERR1631970.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631970.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584973
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26060.16441920461736573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22360.14107495837468526No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22040.13905599653748046No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA21690.1368477570280377No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA21380.13489188774824556No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18370.11590102796703794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14300.031.8251721
CGAATTA450.003826438220.55555515
CTTATAC13900.019.56474937
GCGGTAA8800.018.92045423
TAAACCG902.1532633E-618.55
ATACGGC1601.8189894E-1218.529
GTATCAA25650.017.598442
AACTCCG10050.017.4875625
GCTAACT9800.017.3673462
CGCGGTA10050.017.30348222
TAACTCC10050.017.3034824
CTAACTC9850.017.2791863
CCGCTTA752.0678889E-417.26666625
TAATACG9400.017.1223427
CGGTAAT9750.017.07692324
CGTGCCA10400.016.89903810
CGGGTAA5750.016.73043423
GGTAATA10200.016.68627425
AAGTTCG1005.88062E-616.6533
AACGTCA5550.016.33333428
Produced by FastQC (version 0.11.5)