##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631970.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1584973
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65012463934717	34.0	31.0	34.0	31.0	34.0
2	32.810409388677286	34.0	31.0	34.0	31.0	34.0
3	32.91117955952562	34.0	31.0	34.0	31.0	34.0
4	36.33220376624712	37.0	37.0	37.0	35.0	37.0
5	36.24625908453961	37.0	37.0	37.0	35.0	37.0
6	36.29026677426051	37.0	37.0	37.0	35.0	37.0
7	36.274564929497224	37.0	37.0	37.0	35.0	37.0
8	36.257410063136724	37.0	37.0	37.0	35.0	37.0
9	38.08866334000642	39.0	38.0	39.0	37.0	39.0
10	38.01872019270991	39.0	38.0	39.0	35.0	39.0
11	38.09495429890604	39.0	38.0	39.0	37.0	39.0
12	38.034495855765364	39.0	38.0	39.0	35.0	39.0
13	38.08016792715081	39.0	38.0	39.0	37.0	39.0
14	39.50410196262018	41.0	39.0	41.0	37.0	41.0
15	39.494766156899836	41.0	39.0	41.0	37.0	41.0
16	39.46041225938865	41.0	39.0	41.0	37.0	41.0
17	39.42704765317769	41.0	39.0	41.0	37.0	41.0
18	39.446630952073	41.0	39.0	41.0	37.0	41.0
19	39.472727926595596	41.0	39.0	41.0	37.0	41.0
20	39.456600207069776	41.0	39.0	41.0	37.0	41.0
21	39.42630379192579	41.0	39.0	41.0	37.0	41.0
22	39.407612621792296	40.0	39.0	41.0	37.0	41.0
23	39.343748442402486	40.0	39.0	41.0	36.0	41.0
24	39.362299547058534	41.0	39.0	41.0	36.0	41.0
25	39.29952055965622	40.0	39.0	41.0	36.0	41.0
26	39.25372798148612	40.0	39.0	41.0	36.0	41.0
27	39.14911168833791	40.0	39.0	41.0	36.0	41.0
28	39.14421759865941	40.0	39.0	41.0	36.0	41.0
29	39.11593951442706	40.0	39.0	41.0	35.0	41.0
30	39.07191857526911	40.0	39.0	41.0	35.0	41.0
31	38.99569708758446	40.0	39.0	41.0	35.0	41.0
32	38.94619529796407	40.0	38.0	41.0	35.0	41.0
33	38.89075523683999	40.0	38.0	41.0	35.0	41.0
34	38.85224290886974	40.0	38.0	41.0	35.0	41.0
35	38.781264412706086	40.0	38.0	41.0	35.0	41.0
36	38.73761635056244	40.0	38.0	41.0	35.0	41.0
37	38.696045295408815	40.0	38.0	41.0	35.0	41.0
38	38.65667806328562	40.0	38.0	41.0	35.0	41.0
39	38.592552680708124	40.0	38.0	41.0	35.0	41.0
40	38.27378952196662	40.0	38.0	41.0	34.0	41.0
41	38.34244936664536	40.0	38.0	41.0	34.0	41.0
42	38.320701993030795	40.0	37.0	41.0	34.0	41.0
43	37.86468980859611	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	2.0
14	0.0
15	1.0
16	2.0
17	5.0
18	7.0
19	20.0
20	45.0
21	105.0
22	187.0
23	337.0
24	655.0
25	1044.0
26	1905.0
27	2977.0
28	4681.0
29	6901.0
30	10108.0
31	13865.0
32	19011.0
33	25998.0
34	38076.0
35	57462.0
36	89363.0
37	162004.0
38	367245.0
39	782966.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.29714133931619	17.005841739890837	12.276802191583075	27.420214729209896
2	19.670114254312217	19.520963448588713	33.89982037548905	26.909101921610024
3	20.194539591526166	20.615366949468537	29.20567101143048	29.984422447574815
4	14.734572765592851	15.616543625664287	34.386958011272114	35.26192559747074
5	16.37586255412553	33.79414034182286	33.534956115971696	16.295040988079922
6	34.63958061115237	34.56279696878117	15.292563343350329	15.505059076716133
7	30.796360568918207	28.694873666617664	20.13397073641002	20.374795028054105
8	27.538197811571553	32.36086671507969	19.561973610906936	20.53896186244182
9	27.847477528008362	13.471207395961951	17.85210221246671	40.82921286356297
10	18.38043928824024	24.424706288372107	30.879074911686192	26.31577951170146
11	38.6243172596631	20.142803694447792	19.95371529988208	21.27916374600703
12	23.056354903206554	23.82009031068668	27.835616127214784	25.287938658891978
13	33.46826728278652	18.22699818861268	21.955768331700288	26.348966196900513
14	23.57724705720539	19.26297798132839	24.12867600899195	33.03109895247427
15	27.834732831411007	24.922506566357914	21.039222750166722	26.203537852064358
16	25.488257528677146	24.430952451555957	22.87149370998749	27.209296309779408
17	25.91690836373869	24.256690807982217	23.152823423490496	26.673577404788603
18	26.242907607889848	22.795719548534894	24.12482736298978	26.83654548058547
19	27.43523075787411	23.09219147581694	23.937379374916798	25.535198391392157
20	28.02337957807483	22.399245917753806	23.5962379169866	25.981136587184768
21	26.73080235436187	23.21862896087189	23.259702215747524	26.790866469018717
22	27.09339528181237	23.65787934557876	22.71798951780251	26.53073585480636
23	27.045634215850995	23.117680869011647	23.54311398364515	26.293570931492212
24	26.852066249708983	22.932630398120345	23.24039589317925	26.974907458991414
25	27.004687146090184	22.991180291399285	23.912331629623974	26.091800932886557
26	26.811623920407477	23.43005212076168	23.544249649678576	26.21407430915227
27	26.39369882010608	23.057301291567743	24.04381651927194	26.505183369054237
28	26.048645623616302	23.702675061341736	23.365382249413713	26.88329706562825
29	26.49023043294744	23.938010300490923	23.33629658044648	26.235462686115156
30	26.243664718578803	23.318567571813524	24.108801853406966	26.328965856200703
31	26.717174361960737	23.228786862615326	23.512829556087077	26.54120921933686
32	25.07613694365772	23.33743224647991	23.91605409051132	27.670376719351054
33	25.30888538795298	22.99654315877936	25.13714744667575	26.557424006591912
34	25.827443117327554	22.668777323020645	24.765406098400415	26.738373461251392
35	25.38093708851823	23.747660054776958	24.879919090104373	25.991483766600442
36	25.731731707732557	23.002410766618738	25.018091790838078	26.247765734810624
37	25.714255069329255	22.574264672016493	25.00181391102561	26.709666347628634
38	25.05670443597462	22.26864432390962	25.675642424192713	26.999008815923048
39	24.56723237556728	21.64781355896914	26.627772208107015	27.157181857356562
40	24.59713824778088	22.260757754233037	27.037053628042873	26.10505036994321
41	23.679709370443536	21.569326417547806	27.848991749386265	26.901972462622393
42	22.658051588260495	22.16107151352105	28.386603431099456	26.794273467119
43	22.21438472453474	21.994065514049765	28.432913368240342	27.35863639317515
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	19.0
2	21.0
3	87.5
4	154.0
5	154.0
6	200.0
7	246.0
8	247.5
9	249.0
10	379.5
11	510.0
12	510.0
13	1039.5
14	1569.0
15	3082.0
16	4595.0
17	4657.0
18	4719.0
19	4719.0
20	5289.0
21	5859.0
22	5418.5
23	4978.0
24	5930.0
25	6882.0
26	6882.0
27	8313.5
28	9745.0
29	12429.5
30	15114.0
31	18455.0
32	21796.0
33	21796.0
34	27110.5
35	32425.0
36	37364.0
37	42303.0
38	49291.5
39	56280.0
40	56280.0
41	63827.0
42	71374.0
43	80828.0
44	90282.0
45	103517.5
46	116753.0
47	116753.0
48	124170.5
49	131588.0
50	139829.5
51	148071.0
52	147876.0
53	147681.0
54	147681.0
55	140090.0
56	132499.0
57	125256.5
58	118014.0
59	112778.0
60	107542.0
61	107542.0
62	99175.0
63	90808.0
64	78487.5
65	66167.0
66	58572.5
67	50978.0
68	50978.0
69	44327.5
70	37677.0
71	31918.5
72	26160.0
73	21333.0
74	16506.0
75	16506.0
76	13527.0
77	10548.0
78	8531.0
79	6514.0
80	5268.0
81	4022.0
82	4022.0
83	3090.5
84	2159.0
85	1686.5
86	1214.0
87	912.0
88	610.0
89	610.0
90	442.0
91	274.0
92	164.0
93	54.0
94	34.0
95	14.0
96	14.0
97	7.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1584973.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.52257752287632
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.00345577947475	49.471404523101015
2	7.649724522630278	8.800637437632963
3	2.2913387607829883	3.954111344949323
4	1.1025921016214726	2.5369575856652915
5	0.6447492711599575	1.854381996655833
6	0.4208045104562564	1.4523456044817615
7	0.2944126219916909	1.1854761010561223
8	0.2108821898548315	0.9704389691294774
9	0.15586980587408014	0.8069429692680711
>10	1.0437602030824384	11.522237582572101
>50	0.09886363664389493	4.002833353139128
>100	0.07431265690068331	8.599065045066597
>500	0.006595671090435551	2.629431711192572
>1k	0.0026382684361742205	2.213735776089766
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2606	0.16441920461736573	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2236	0.14107495837468526	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2204	0.13905599653748046	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2169	0.1368477570280377	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2138	0.13489188774824556	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1837	0.11590102796703794	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	6.309255741264993E-5	0.0	0.0	0.0	0.0
6	6.309255741264993E-5	0.0	0.0	0.0	0.0
7	6.309255741264993E-5	0.0	0.0	0.0	0.0
8	6.309255741264993E-5	0.0	0.0	0.0	0.0
9	6.309255741264993E-5	0.0	0.0	6.309255741264993E-5	0.0
10	6.309255741264993E-5	0.0	0.0	6.309255741264993E-5	0.0
11	6.309255741264993E-5	0.0	0.0	1.2618511482529986E-4	0.0
12	6.309255741264993E-5	0.0	0.0	1.2618511482529986E-4	0.0
13	6.309255741264993E-5	0.0	0.0	1.892776722379498E-4	0.0
14	6.309255741264993E-5	0.0	0.0	2.523702296505997E-4	0.0
15	6.309255741264993E-5	0.0	0.0	3.785553444758996E-4	0.0
16	6.309255741264993E-5	0.0	0.0	5.678330167138494E-4	0.0
17	6.309255741264993E-5	0.0	0.0	8.83295803777099E-4	0.0
18	6.309255741264993E-5	0.0	0.0	9.46388361189749E-4	0.0
19	6.309255741264993E-5	0.0	0.0	0.0013249437056656486	0.0
20	1.2618511482529986E-4	0.0	0.0	0.001703499050141548	0.0
21	1.2618511482529986E-4	0.0	0.0	0.0032177204280451465	0.0
22	1.2618511482529986E-4	0.0	0.0	0.005425959937487894	0.0
23	1.2618511482529986E-4	0.0	0.0	0.006687811085740893	0.0
24	1.2618511482529986E-4	0.0	0.0	0.008012754791406541	0.0
25	1.2618511482529986E-4	0.0	0.0	0.008896050595183641	0.0
26	1.2618511482529986E-4	0.0	0.0	0.010978104989801087	0.0
27	1.2618511482529986E-4	6.309255741264993E-5	0.0	0.023912079259394325	6.309255741264993E-5
28	1.2618511482529986E-4	6.309255741264993E-5	0.0	0.0977934639896074	1.2618511482529986E-4
29	1.2618511482529986E-4	6.309255741264993E-5	0.0	0.21640747192538926	1.892776722379498E-4
30	1.2618511482529986E-4	6.309255741264993E-5	0.0	0.36524281486183047	1.892776722379498E-4
31	1.2618511482529986E-4	6.309255741264993E-5	0.0	0.858248058484277	1.892776722379498E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1430	0.0	31.825172	1
CGAATTA	45	0.0038264382	20.555555	15
CTTATAC	1390	0.0	19.564749	37
GCGGTAA	880	0.0	18.920454	23
TAAACCG	90	2.1532633E-6	18.5	5
ATACGGC	160	1.8189894E-12	18.5	29
GTATCAA	2565	0.0	17.59844	2
AACTCCG	1005	0.0	17.487562	5
GCTAACT	980	0.0	17.367346	2
CGCGGTA	1005	0.0	17.303482	22
TAACTCC	1005	0.0	17.303482	4
CTAACTC	985	0.0	17.279186	3
CCGCTTA	75	2.0678889E-4	17.266666	25
TAATACG	940	0.0	17.12234	27
CGGTAAT	975	0.0	17.076923	24
CGTGCCA	1040	0.0	16.899038	10
CGGGTAA	575	0.0	16.730434	23
GGTAATA	1020	0.0	16.686274	25
AAGTTCG	100	5.88062E-6	16.65	33
AACGTCA	555	0.0	16.333334	28
>>END_MODULE