FastQCFastQC Report
Fri 10 Feb 2017
ERR1631969.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631969.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271281
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT65490.5151496797324904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT54360.4276001922470327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52620.4139132103759908No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA27170.21372143530816556No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA27010.21246286226255248No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA24380.19177506782528803No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA23860.18768470542704563No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG23180.18233576998319018No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC22910.18021192796871816No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA21060.1656596771288173No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA21060.1656596771288173No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT21020.16534503386741406No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC19540.15370323319549337No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA18790.1478036720441822No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT17710.13930830398629412No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA14730.11586738101175113No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG14430.11350755655122667No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT14150.11130505372140384No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13550.10658540480035493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24950.027.7314621
GACCGTT2000.020.3499987
ATACGGC1001.2900273E-820.34999829
AATATAG759.267038E-619.7333345
CTTATAC18000.019.32222237
GTACTAT1254.129106E-1019.241
ACTGTGC1950.018.9743588
TTTAGGA1501.2732926E-1118.52
CGTTATT1501.2732926E-1118.510
TACTATT500.007035284318.4999982
CTCTATG7050.017.8439731
TTAGGAC4150.017.8313263
GCAGTCG3750.017.7599989
ACCTAGC953.606996E-617.5263161
TCCGTCG953.606996E-617.52631610
TAAGTCT1603.45608E-1117.3437535
TGTTACG1603.45608E-1117.3437516
TATAGAC752.0674833E-417.2666662
GCGGTAA9550.017.24083723
TCTATGG7300.017.2328762
Produced by FastQC (version 0.11.5)