##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631969.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1271281
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57053711964546	34.0	31.0	34.0	31.0	34.0
2	32.734985420217875	34.0	31.0	34.0	31.0	34.0
3	32.822501870160885	34.0	31.0	34.0	31.0	34.0
4	36.26264924906452	37.0	37.0	37.0	35.0	37.0
5	36.18405057575784	37.0	35.0	37.0	35.0	37.0
6	36.23948363894371	37.0	37.0	37.0	35.0	37.0
7	36.22698994164154	37.0	37.0	37.0	35.0	37.0
8	36.21012506283033	37.0	36.0	37.0	35.0	37.0
9	38.03786888972619	39.0	38.0	39.0	35.0	39.0
10	37.97344725517018	39.0	38.0	39.0	35.0	39.0
11	38.04424120237776	39.0	38.0	39.0	35.0	39.0
12	37.9780489128682	39.0	38.0	39.0	35.0	39.0
13	38.03052511600504	39.0	38.0	39.0	35.0	39.0
14	39.390457341846535	40.0	39.0	41.0	37.0	41.0
15	39.39096627732185	40.0	39.0	41.0	37.0	41.0
16	39.34151536914341	40.0	39.0	41.0	36.0	41.0
17	39.324066040474136	40.0	39.0	41.0	36.0	41.0
18	39.357721856930134	40.0	39.0	41.0	36.0	41.0
19	39.37978228259527	40.0	39.0	41.0	37.0	41.0
20	39.37848123270937	40.0	39.0	41.0	36.0	41.0
21	39.34521636050566	40.0	39.0	41.0	36.0	41.0
22	39.31239670851684	40.0	39.0	41.0	36.0	41.0
23	39.24790191940256	40.0	39.0	41.0	36.0	41.0
24	39.258718568121445	40.0	39.0	41.0	36.0	41.0
25	39.199947139932085	40.0	39.0	41.0	36.0	41.0
26	39.12294449456886	40.0	39.0	41.0	36.0	41.0
27	39.01368068900582	40.0	39.0	41.0	35.0	41.0
28	39.002138787569386	40.0	39.0	41.0	35.0	41.0
29	38.97164828232311	40.0	38.0	41.0	35.0	41.0
30	38.89646270179449	40.0	38.0	41.0	35.0	41.0
31	38.791749424399484	40.0	38.0	41.0	35.0	41.0
32	38.73035937766709	40.0	38.0	41.0	35.0	41.0
33	38.64865045572143	40.0	38.0	41.0	35.0	41.0
34	38.602507234828494	40.0	38.0	41.0	35.0	41.0
35	38.50997458469056	40.0	38.0	41.0	35.0	41.0
36	38.45354567558235	40.0	38.0	41.0	35.0	41.0
37	38.39072872165949	40.0	38.0	41.0	35.0	41.0
38	38.34253009366143	40.0	38.0	41.0	34.0	41.0
39	38.24881674468509	40.0	38.0	41.0	34.0	41.0
40	37.91902341024526	40.0	37.0	41.0	33.0	41.0
41	37.95111466308393	40.0	37.0	41.0	33.0	41.0
42	37.89517187781458	40.0	37.0	41.0	33.0	41.0
43	37.40078472029394	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	0.0
13	0.0
14	2.0
15	1.0
16	1.0
17	1.0
18	10.0
19	18.0
20	34.0
21	86.0
22	176.0
23	367.0
24	636.0
25	1093.0
26	1798.0
27	3025.0
28	4668.0
29	6897.0
30	9673.0
31	13250.0
32	17557.0
33	23540.0
34	34118.0
35	51152.0
36	77049.0
37	146016.0
38	300869.0
39	579240.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.064121936849524	16.634638604682994	13.011206806363033	27.29003265210445
2	19.816940550515582	19.742370097563008	34.032523100714954	26.40816625120646
3	20.529056911886514	20.77762508839509	29.849498261989282	28.84381973772911
4	14.643812028969204	16.645572458016755	34.85382067379281	33.85679483922123
5	16.204678587975437	33.56692973465347	33.717014570342826	16.511377107028267
6	32.4120316436728	34.96937341154316	16.122084731857083	16.49651021292696
7	29.566948613249156	28.86537280113523	20.696289805322348	20.871388780293262
8	27.281222640785163	31.527097470976123	20.14959713863418	21.042082749604532
9	28.525007453112256	13.202667230926915	18.48159454912014	39.790730766840696
10	19.36998979769225	23.99288591585967	29.60753759397018	27.029586692477903
11	37.785037297025596	20.487602662196636	19.686835561925335	22.040524478852433
12	22.620490670433995	24.70736210169113	27.365468374025887	25.30667885384899
13	33.89140559797559	18.327498011847894	21.96705527731477	25.81404111286175
14	23.267711859140505	20.7784116965486	25.09358670506363	30.860289739247264
15	28.839572053700163	24.811115717138854	21.521127115090998	24.82818511406998
16	24.067613690442947	24.95522233086155	23.80339201167956	27.17377196701595
17	25.49868990412033	24.685494395023603	23.375870480247876	26.43994522060819
18	25.575384199087374	22.09448579818309	25.07840516770092	27.251724835028607
19	26.766466265129424	23.121481403403337	25.389980657305504	24.72207167416173
20	28.153571083025703	21.709362446225498	24.939332846160685	25.197733624588114
21	26.36246431748764	23.081207065943723	24.129913056200795	26.42641556036785
22	27.1690523180949	23.738496839015134	23.582984407066572	25.50946643582339
23	26.445844781759504	23.081364387574425	24.697293517326223	25.77549731333985
24	26.347912066647734	22.740920378736092	24.928634975272974	25.9825325793432
25	26.111536316518535	23.016626536540702	25.342626846464317	25.529210300476446
26	26.24242791326229	23.73613701455461	24.789090688840627	25.23234438334247
27	25.844954813294624	22.940246884835062	25.859192420873118	25.3556058809972
28	25.032781894797452	23.68013051402483	24.52888071166013	26.758206879517587
29	25.897657559579667	24.062500737445145	24.70366504336964	25.33617665960555
30	25.42427677279846	22.94480921212541	25.81168128840123	25.819232726674908
31	26.09179245186548	23.32332505559353	25.017443035804042	25.567439456736945
32	24.166568996154272	23.295715109405396	25.276237118308227	27.26147877613211
33	24.381234361246648	23.09347815313845	26.762375902731183	25.762911582883717
34	25.072898910626368	22.876610285216252	26.076374932056723	25.97411587210066
35	24.4441630135273	24.16177068641787	26.33658490923722	25.05748139081761
36	24.914476028509828	23.144371700670426	26.3345397280381	25.606612542781647
37	24.845018528555055	23.060283289060404	26.168329425201826	25.926368757182715
38	24.66873964135388	22.20319504499792	26.895470002304762	26.23259531134344
39	23.906359018973774	21.546770540895366	27.950940822682004	26.595929617448853
40	23.676040151626587	22.447436876662202	28.582272526687646	25.294250445023565
41	22.244885277133854	21.81531856450305	29.261665988872643	26.678130169490462
42	20.86816368686388	22.618838793311628	29.71074058371045	26.802256936114045
43	20.535035133853178	22.91814319572148	29.51542577919437	27.031395891230968
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	55.0
1	53.5
2	52.0
3	150.5
4	249.0
5	249.0
6	372.5
7	496.0
8	533.0
9	570.0
10	919.0
11	1268.0
12	1268.0
13	2428.0
14	3588.0
15	7303.0
16	11018.0
17	10632.0
18	10246.0
19	10246.0
20	10785.0
21	11324.0
22	8696.0
23	6068.0
24	5949.5
25	5831.0
26	5831.0
27	6340.0
28	6849.0
29	8194.5
30	9540.0
31	11486.5
32	13433.0
33	13433.0
34	17327.0
35	21221.0
36	23874.5
37	26528.0
38	31975.5
39	37423.0
40	37423.0
41	43500.0
42	49577.0
43	58124.0
44	66671.0
45	84250.5
46	101830.0
47	101830.0
48	111915.5
49	122001.0
50	133753.0
51	145505.0
52	142411.5
53	139318.0
54	139318.0
55	125663.5
56	112009.0
57	102022.0
58	92035.0
59	84439.5
60	76844.0
61	76844.0
62	70356.5
63	63869.0
64	51958.5
65	40048.0
66	34725.0
67	29402.0
68	29402.0
69	25023.5
70	20645.0
71	18035.0
72	15425.0
73	14077.5
74	12730.0
75	12730.0
76	10816.0
77	8902.0
78	6494.0
79	4086.0
80	3019.5
81	1953.0
82	1953.0
83	1611.5
84	1270.0
85	1015.5
86	761.0
87	566.5
88	372.0
89	372.0
90	281.5
91	191.0
92	128.0
93	65.0
94	38.5
95	12.0
96	12.0
97	6.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1271281.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.810855479234334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.66370502602483	34.55198230153625
2	7.094904975736297	5.790982832073492
3	2.374578206511279	2.9072570403021376
4	1.256870123035697	2.051757797895092
5	0.8075246840488222	1.6477886588315425
6	0.531701608105512	1.3019518491882325
7	0.3849822849663974	1.0998019475680338
8	0.3006592544257285	0.9816129104690201
9	0.23830377053675444	0.8752842665578896
>10	1.8610210423869984	15.220577853133172
>50	0.2643811622766849	7.614281520685806
>100	0.1975461237475776	15.707830669044565
>500	0.015881158798310147	4.361081948525366
>1k	0.007359561394338848	4.524614617647793
>5k	5.810180048162249E-4	1.363193786541558
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6549	0.5151496797324904	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5436	0.4276001922470327	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5262	0.4139132103759908	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2717	0.21372143530816556	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2701	0.21246286226255248	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2438	0.19177506782528803	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	2386	0.18768470542704563	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	2318	0.18233576998319018	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	2291	0.18021192796871816	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2106	0.1656596771288173	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2106	0.1656596771288173	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	2102	0.16534503386741406	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1954	0.15370323319549337	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1879	0.1478036720441822	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1771	0.13930830398629412	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1473	0.11586738101175113	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1443	0.11350755655122667	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1415	0.11130505372140384	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1355	0.10658540480035493	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.866081535081544E-5
2	0.0	0.0	0.0	0.0	7.866081535081544E-5
3	0.0	0.0	0.0	0.0	7.866081535081544E-5
4	0.0	0.0	0.0	0.0	7.866081535081544E-5
5	0.0	0.0	0.0	0.0	7.866081535081544E-5
6	0.0	0.0	0.0	0.0	7.866081535081544E-5
7	0.0	0.0	0.0	0.0	7.866081535081544E-5
8	7.866081535081544E-5	0.0	0.0	0.0	7.866081535081544E-5
9	7.866081535081544E-5	0.0	0.0	0.0	7.866081535081544E-5
10	7.866081535081544E-5	0.0	0.0	0.0	7.866081535081544E-5
11	7.866081535081544E-5	0.0	0.0	7.866081535081544E-5	7.866081535081544E-5
12	7.866081535081544E-5	0.0	0.0	3.933040767540772E-4	7.866081535081544E-5
13	7.866081535081544E-5	0.0	0.0	4.719648921048926E-4	7.866081535081544E-5
14	7.866081535081544E-5	0.0	0.0	9.439297842097853E-4	7.866081535081544E-5
15	1.5732163070163088E-4	0.0	0.0	0.0013372338609638624	7.866081535081544E-5
16	2.359824460524463E-4	0.0	0.0	0.0027531285372785403	7.866081535081544E-5
17	2.359824460524463E-4	0.0	0.0	0.003775719136839141	7.866081535081544E-5
18	2.359824460524463E-4	0.0	0.0	0.004798309736399741	7.866081535081544E-5
19	2.359824460524463E-4	0.0	0.0	0.006214204412714419	7.866081535081544E-5
20	3.1464326140326175E-4	0.0	0.0	0.00810206398113399	7.866081535081544E-5
21	3.1464326140326175E-4	0.0	0.0	0.013765642686392701	1.5732163070163088E-4
22	3.1464326140326175E-4	0.0	0.0	0.019035917314897337	2.359824460524463E-4
23	3.1464326140326175E-4	0.0	0.0	0.022811636451736478	2.359824460524463E-4
24	3.1464326140326175E-4	0.0	0.0	0.02705932048068051	2.359824460524463E-4
25	3.1464326140326175E-4	0.0	0.0	0.029104501679801713	2.359824460524463E-4
26	3.933040767540772E-4	0.0	0.0	0.03453209793900798	2.359824460524463E-4
27	3.933040767540772E-4	0.0	0.0	0.05514123156092162	2.359824460524463E-4
28	3.933040767540772E-4	0.0	0.0	0.1691207530042532	2.359824460524463E-4
29	4.719648921048926E-4	0.0	0.0	0.3511418797260401	2.359824460524463E-4
30	4.719648921048926E-4	0.0	0.0	0.5778423495670902	2.359824460524463E-4
31	4.719648921048926E-4	0.0	0.0	1.2447287421113036	2.359824460524463E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2495	0.0	27.731462	1
GACCGTT	200	0.0	20.349998	7
ATACGGC	100	1.2900273E-8	20.349998	29
AATATAG	75	9.267038E-6	19.733334	5
CTTATAC	1800	0.0	19.322222	37
GTACTAT	125	4.129106E-10	19.24	1
ACTGTGC	195	0.0	18.974358	8
TTTAGGA	150	1.2732926E-11	18.5	2
CGTTATT	150	1.2732926E-11	18.5	10
TACTATT	50	0.0070352843	18.499998	2
CTCTATG	705	0.0	17.843973	1
TTAGGAC	415	0.0	17.831326	3
GCAGTCG	375	0.0	17.759998	9
ACCTAGC	95	3.606996E-6	17.526316	1
TCCGTCG	95	3.606996E-6	17.526316	10
TAAGTCT	160	3.45608E-11	17.34375	35
TGTTACG	160	3.45608E-11	17.34375	16
TATAGAC	75	2.0674833E-4	17.266666	2
GCGGTAA	955	0.0	17.240837	23
TCTATGG	730	0.0	17.232876	2
>>END_MODULE