FastQCFastQC Report
Fri 10 Feb 2017
ERR1631968.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631968.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116680
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29940.26811620159759286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28530.25548948669269617No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23130.20713185514202814No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC22330.19996776157896623No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA20370.18241573234946448No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA17310.1550130744707526No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT16220.1452519969910807No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14910.13352079378156678No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA13530.12116273238528495No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA13170.11793889028190707No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12490.11184941075330443No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA11960.10710319876777591No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG11400.10208833327363255No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGT250.00549627829.5999986
AGACCGT405.93998E-527.7500026
GGTATCA14800.027.3751
CTTACAT358.869234E-426.4285723
ATAGAGC952.8194336E-1023.36842232
ATACGGC1200.023.12500229
GTTATCG400.001931152723.12500211
TATTAGA2500.021.462
ATTAGAG2450.021.1428573
ATACACA2700.019.87036937
CTTATAC12250.019.78367437
GGACGTA1205.173206E-918.527
ACCTCTA1700.018.49999831
CGTTACG500.007034760418.49999816
TAACCCA500.007034760418.4999984
TATCCGG500.007034760418.49999810
ATGCTAG1800.017.47222115
TATACAC4050.017.35802537
GTGACAC3000.017.26666624
ATAATCG650.001579895817.07692315
Produced by FastQC (version 0.11.5)