FastQCFastQC Report
Fri 10 Feb 2017
ERR1631967.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631967.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405636
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8880.2189154808744786No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8640.21299884625624946No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8260.20363084144405327No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8230.20289126211677466No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7320.1804573558559891No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7140.17601987989231727No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7090.1747872476801862No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6830.1683775601771046No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6690.16492618998313757No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6510.16048871401946574No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6370.15703734382549872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6280.1548186058436628No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5970.14717628612845013No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5800.1429853366072045No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5710.14076659862536856No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5120.12622153852222187No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5020.12375627409795975No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC4620.11389521640091116No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4560.11241605774635387No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4530.11167647841907524No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4310.10625289668569851No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4280.10551331735841986No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4160.10255500004930529No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4150.10230847360687909No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4120.10156889427960043No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC4080.10058278850989556No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4080.10058278850989556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6250.031.3761
CTATAAG250.00549220329.62
ATACGGC509.072162E-625.90000229
TACGGCT551.8987257E-523.54545430
CCGGTGG400.001929045223.12515
GACCGTT652.6757443E-622.769237
CCGTTTA603.718638E-521.58333427
CTCCACT450.00382160520.5555552
ATATACA450.00382160520.5555554
AGTTCGC450.00382160520.5555558
AATAGAC450.00382160520.55555518
TATACAA450.00382160520.5555555
GTATCAA9850.019.7208142
TCTTATA10550.019.63980937
CCGTCGC500.00702722718.518
TTATACA2800.018.537
TATCTGG701.2167302E-418.51
CTAAAGC500.00702722718.533
TTCGCTT609.2227734E-418.510
TACCGGT500.00702722718.513
Produced by FastQC (version 0.11.5)