##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631967.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	405636
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57384206529992	34.0	31.0	34.0	31.0	34.0
2	32.74200268220769	34.0	31.0	34.0	31.0	34.0
3	32.8294283544853	34.0	31.0	34.0	31.0	34.0
4	36.2723846009723	37.0	37.0	37.0	35.0	37.0
5	36.19182962064511	37.0	35.0	37.0	35.0	37.0
6	36.24048654458677	37.0	37.0	37.0	35.0	37.0
7	36.23211943713083	37.0	37.0	37.0	35.0	37.0
8	36.21268082714552	37.0	36.0	37.0	35.0	37.0
9	38.045328816968905	39.0	38.0	39.0	35.0	39.0
10	37.97974538749026	39.0	38.0	39.0	35.0	39.0
11	38.055981224546144	39.0	38.0	39.0	35.0	39.0
12	37.98708941021014	39.0	38.0	39.0	35.0	39.0
13	38.03816722381643	39.0	38.0	39.0	35.0	39.0
14	39.40521058288712	40.0	39.0	41.0	37.0	41.0
15	39.41226863493378	40.0	39.0	41.0	37.0	41.0
16	39.34839363370115	40.0	39.0	41.0	36.0	41.0
17	39.32844964450887	40.0	39.0	41.0	36.0	41.0
18	39.359664822648874	40.0	39.0	41.0	36.0	41.0
19	39.3842262521078	40.0	39.0	41.0	37.0	41.0
20	39.37994162253843	40.0	39.0	41.0	37.0	41.0
21	39.34403504619905	40.0	39.0	41.0	36.0	41.0
22	39.3122257393328	40.0	39.0	41.0	36.0	41.0
23	39.25543837331992	40.0	39.0	41.0	36.0	41.0
24	39.26120216154385	40.0	39.0	41.0	36.0	41.0
25	39.206142945892374	40.0	39.0	41.0	36.0	41.0
26	39.145421017858375	40.0	39.0	41.0	36.0	41.0
27	39.032248123933776	40.0	39.0	41.0	35.0	41.0
28	39.0308281316254	40.0	39.0	41.0	35.0	41.0
29	39.007437702768	40.0	39.0	41.0	35.0	41.0
30	38.95951296236034	40.0	38.0	41.0	35.0	41.0
31	38.87645573864253	40.0	38.0	41.0	35.0	41.0
32	38.82988935893264	40.0	38.0	41.0	35.0	41.0
33	38.7688987170764	40.0	38.0	41.0	35.0	41.0
34	38.74584602944512	40.0	38.0	41.0	35.0	41.0
35	38.67891903085525	40.0	38.0	41.0	35.0	41.0
36	38.62747635811417	40.0	38.0	41.0	35.0	41.0
37	38.58684386987348	40.0	38.0	41.0	35.0	41.0
38	38.5522882584386	40.0	38.0	41.0	35.0	41.0
39	38.485486988334365	40.0	38.0	41.0	35.0	41.0
40	38.1929463854293	40.0	38.0	41.0	34.0	41.0
41	38.25775325661431	40.0	37.0	41.0	34.0	41.0
42	38.23844284037906	40.0	37.0	41.0	34.0	41.0
43	37.76702265084953	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	4.0
20	13.0
21	21.0
22	49.0
23	101.0
24	199.0
25	319.0
26	537.0
27	834.0
28	1290.0
29	1993.0
30	2745.0
31	3774.0
32	5130.0
33	7068.0
34	10260.0
35	15765.0
36	24338.0
37	43805.0
38	98228.0
39	189160.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.45949570550937	16.870050981668292	13.127286532753505	27.54316678006883
2	20.37467088719936	19.912680334092634	33.46990898243746	26.24273979627055
3	20.872407774457887	20.302685166010907	30.14796517074421	28.67694188878699
4	14.961689790846966	16.55572976757487	35.166503959214666	33.3160764823635
5	16.34075870977921	33.401621157885394	33.81677168693114	16.440848445404256
6	32.21459633760317	34.955230798055396	16.154138192862565	16.676034671478863
7	29.23433817511266	28.698882742162922	20.541815815164334	21.52496326756008
8	26.614501671449283	31.39662160163299	20.73632517823862	21.25255154867911
9	28.107465806782432	13.37110118431303	18.262432328491553	40.25900068041298
10	19.13735467266219	23.91824197063377	29.91228589178475	27.032117464919285
11	38.29665019870031	20.2509146131014	19.489147906004398	21.96328728219389
12	22.116133676498144	24.676557307536807	27.30033823427901	25.906970781686045
13	34.318946050153336	18.302123085722176	21.68002839984617	25.698902464278316
14	23.343588833338263	21.052372077429023	24.223441706357423	31.380597382875287
15	29.120936011596605	24.9041012138962	20.856630082142612	25.118332692364582
16	24.39822895403761	24.996302103363607	23.312773028034986	27.292695914563797
17	26.07066433945705	24.52716228342652	22.612391405102112	26.78978197201432
18	25.88429034898283	21.89204114033271	24.296413533315583	27.92725497736887
19	27.55771184017198	22.98809770335966	24.266830360224436	25.18736009624392
20	29.030460807226184	21.41723121221982	23.897780275912396	25.6545277046416
21	26.681556863789208	23.142423256318473	22.87420248695875	27.301817392933565
22	27.509392657456438	23.72743050419588	22.27834807561459	26.48482876273309
23	27.103610133222887	22.81380350856433	23.4323383526117	26.650248005601078
24	26.892336972063625	22.31951799149977	23.90788786005187	26.880257176384738
25	26.9635831139248	22.709769349860466	24.2034730645209	26.123174471693833
26	27.065891587531677	23.48460195840606	23.332002090544233	26.11750436351803
27	26.28317013282845	22.461763748779696	24.927521225926693	26.327544892465166
28	25.641215276750586	23.68576753542585	23.176197378930862	27.496819808892702
29	26.431825577611455	24.239958977999983	23.32238755928961	26.005827885098952
30	25.94961985622578	22.877900383595147	24.816091273950043	26.356388486229033
31	26.91427782543956	22.980701910086875	23.95472788411285	26.150292380360717
32	24.627252019051564	23.024583616838743	24.128282499580905	28.21988186452879
33	24.80327189894388	22.476801861767694	25.946168486031812	26.773757753256618
34	25.26674161070516	22.588231813744343	25.290654675620505	26.854371899929987
35	24.989152836533247	23.990967271149504	25.456566971373352	25.563312920943897
36	25.21497105779566	22.75463716238204	25.971314183159283	26.059077596663016
37	25.794308197497262	22.263803015511442	25.56208028873177	26.37980849825952
38	24.96548629806033	21.664497233973314	26.59108165941879	26.778934808547568
39	24.3097259612066	20.656450610892524	28.019455866836278	27.0143675610646
40	24.085879951483598	21.742153063337575	28.47552978532477	25.696437199854056
41	22.377205179027502	20.918015166306738	29.498861047835987	27.20591860682977
42	20.93625812304628	21.4226547939532	30.429744894437377	27.211342188563147
43	20.227001548186056	22.153359169304498	30.01656657693104	27.603072705578402
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11.0
1	9.0
2	7.0
3	20.0
4	33.0
5	33.0
6	39.0
7	45.0
8	54.5
9	64.0
10	93.0
11	122.0
12	122.0
13	270.5
14	419.0
15	863.5
16	1308.0
17	1233.5
18	1159.0
19	1159.0
20	1368.5
21	1578.0
22	1367.5
23	1157.0
24	1309.5
25	1462.0
26	1462.0
27	1660.0
28	1858.0
29	2559.5
30	3261.0
31	3765.0
32	4269.0
33	4269.0
34	5311.5
35	6354.0
36	7028.0
37	7702.0
38	9603.0
39	11504.0
40	11504.0
41	13835.0
42	16166.0
43	18239.5
44	20313.0
45	26628.0
46	32943.0
47	32943.0
48	36598.5
49	40254.0
50	44305.0
51	48356.0
52	48409.0
53	48462.0
54	48462.0
55	42843.5
56	37225.0
57	34380.0
58	31535.0
59	28973.0
60	26411.0
61	26411.0
62	23548.0
63	20685.0
64	16806.0
65	12927.0
66	11151.5
67	9376.0
68	9376.0
69	7970.0
70	6564.0
71	5677.5
72	4791.0
73	3782.5
74	2774.0
75	2774.0
76	2268.5
77	1763.0
78	1448.0
79	1133.0
80	903.0
81	673.0
82	673.0
83	584.0
84	495.0
85	368.5
86	242.0
87	189.5
88	137.0
89	137.0
90	106.0
91	75.0
92	47.5
93	20.0
94	11.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	405636.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.41044360411419
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.31232477290914	40.44695162381882
2	6.464642885335739	6.129831738718963
3	2.315079302018374	3.2927681006218257
4	1.2126568121854775	2.299703896210579
5	0.7902821509790631	1.8733813675165463
6	0.5760231036480056	1.6385706522102355
7	0.41403678571107544	1.3740767375288558
8	0.31313303667651404	1.1876620940749512
9	0.2491311064739486	1.0630274646162337
>10	1.9980567536898763	18.96510538054943
>50	0.214770530358718	7.086181766529473
>100	0.13095764046263283	11.765351523063366
>500	0.008905119551459032	2.8773876545407218
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	888	0.2189154808744786	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	864	0.21299884625624946	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	826	0.20363084144405327	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	823	0.20289126211677466	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	732	0.1804573558559891	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	714	0.17601987989231727	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	709	0.1747872476801862	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	683	0.1683775601771046	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	669	0.16492618998313757	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	651	0.16048871401946574	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	637	0.15703734382549872	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	628	0.1548186058436628	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	597	0.14717628612845013	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	580	0.1429853366072045	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	571	0.14076659862536856	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	512	0.12622153852222187	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	502	0.12375627409795975	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	462	0.11389521640091116	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	456	0.11241605774635387	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	453	0.11167647841907524	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	431	0.10625289668569851	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	428	0.10551331735841986	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	416	0.10255500004930529	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	415	0.10230847360687909	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	412	0.10156889427960043	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	408	0.10058278850989556	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	408	0.10058278850989556	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.4652644242621465E-4	0.0
11	0.0	0.0	0.0	2.4652644242621465E-4	0.0
12	0.0	0.0	0.0	2.4652644242621465E-4	0.0
13	0.0	0.0	0.0	2.4652644242621465E-4	0.0
14	0.0	0.0	0.0	2.4652644242621465E-4	0.0
15	0.0	0.0	0.0	4.930528848524293E-4	0.0
16	0.0	0.0	0.0	0.0012326322121310731	0.0
17	0.0	0.0	0.0	0.0022187379818359317	0.0
18	0.0	0.0	0.0	0.0029583173091145758	0.0
19	0.0	0.0	0.0	0.0036978966363932194	2.4652644242621465E-4
20	0.0	0.0	0.0	0.0051770552909505075	2.4652644242621465E-4
21	0.0	0.0	0.0	0.007888846157638869	2.4652644242621465E-4
22	0.0	0.0	0.0	0.011833269236458303	2.4652644242621465E-4
23	0.0	0.0	0.0	0.014052007218294233	2.4652644242621465E-4
24	0.0	0.0	0.0	0.01602421875770395	2.4652644242621465E-4
25	0.0	0.0	0.0	0.01750337741226124	2.4652644242621465E-4
26	0.0	0.0	0.0	0.022187379818359318	2.4652644242621465E-4
27	0.0	0.0	0.0	0.04610044473370214	2.4652644242621465E-4
28	0.0	0.0	0.0	0.155065132286089	2.4652644242621465E-4
29	0.0	0.0	0.0	0.33059195929355384	2.4652644242621465E-4
30	0.0	0.0	0.0	0.5635594473863267	2.4652644242621465E-4
31	0.0	0.0	2.4652644242621465E-4	1.2055143034641895	2.4652644242621465E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	625	0.0	31.376	1
CTATAAG	25	0.005492203	29.6	2
ATACGGC	50	9.072162E-6	25.900002	29
TACGGCT	55	1.8987257E-5	23.545454	30
CCGGTGG	40	0.0019290452	23.125	15
GACCGTT	65	2.6757443E-6	22.76923	7
CCGTTTA	60	3.718638E-5	21.583334	27
CTCCACT	45	0.003821605	20.555555	2
ATATACA	45	0.003821605	20.555555	4
AGTTCGC	45	0.003821605	20.555555	8
AATAGAC	45	0.003821605	20.555555	18
TATACAA	45	0.003821605	20.555555	5
GTATCAA	985	0.0	19.720814	2
TCTTATA	1055	0.0	19.639809	37
CCGTCGC	50	0.007027227	18.5	18
TTATACA	280	0.0	18.5	37
TATCTGG	70	1.2167302E-4	18.5	1
CTAAAGC	50	0.007027227	18.5	33
TTCGCTT	60	9.2227734E-4	18.5	10
TACCGGT	50	0.007027227	18.5	13
>>END_MODULE