FastQCFastQC Report
Fri 10 Feb 2017
ERR1631966.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631966.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821923
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA26340.3204679757106201No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA25380.3087880494888207No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA24550.29868977994289003No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA24050.29260648503570286No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC20990.2553767202037174No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA19850.24150680781533063No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG19060.2318952018619749No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18130.22058027333460678No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT17330.21084700148310728No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC17090.20792701992765747No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT16870.2052503701684951No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA16790.20427704298334515No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA16450.2001404024464579No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA14050.17094058689195946No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT13890.16899393252165956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13640.165952285068066No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13170.16023398785531004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12880.1567056768091415No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC12550.15269070217039796No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC11520.1401591146615924No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC11350.13809079439314875No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA11330.13784746259686126No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11210.13638747181913635No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11180.1360224741247051No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA10940.13310249256925527No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT10810.13152083589338662No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA10790.13127750409709912No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA10710.13030417691194918No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10710.13030417691194918No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT10670.1298175133193742No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA10630.1293308497267992No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA10620.12920918382865548No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG10470.12738419535649934No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC10320.1255592068843432No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT10090.12276089122703708No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA10000.1216658981437434No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG9910.1205709050604497No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC9510.11570426913469997No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9470.11521760554212498No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC9320.11339261706996885No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC9250.11254095578296264No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT9240.1124192898848189No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT9240.1124192898848189No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC9070.11035096961637526No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG9050.11010763782008776No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC8760.10657932677391921No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG8630.10499767009805054No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA8620.1048760041999068No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA8540.10390267701475685No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC8520.10365934521846937No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8430.10256435213517569No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC8300.100982695459307No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA8260.10049603186673205No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTTA250.00549544429.5999988
GGTATCA16100.028.8416161
TAGCGAA1500.025.910
TAAGGTA1500.025.95
CGTTATT809.786163E-1025.43750210
TGTTACG809.786163E-1025.43750216
TCCTAAG1750.024.3142872
TTATTGT851.9754225E-923.94117512
GTAGCGA1650.023.5454549
TCCATTC400.001930721523.1250023
GTCTAAA400.001930721523.1250022
GTTACTC400.001930721523.12500228
GTACTAT400.001930721523.1250021
ATACGGC1850.023.029
TCGGGTA1700.022.8529425
TAATACT1155.456968E-1222.521744
GACCGTT1500.022.27
CGGGACG603.7246245E-521.58333216
ATTGTTA957.1377144E-921.42105314
GATGCTA957.1377144E-921.42105314
Produced by FastQC (version 0.11.5)