##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631966.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	821923
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.549358029888445	34.0	31.0	34.0	31.0	34.0
2	32.714552336411074	34.0	31.0	34.0	31.0	34.0
3	32.804068020970334	34.0	31.0	34.0	31.0	34.0
4	36.249121876380144	37.0	37.0	37.0	35.0	37.0
5	36.169479379455254	37.0	35.0	37.0	35.0	37.0
6	36.22870268869468	37.0	36.0	37.0	35.0	37.0
7	36.22793132690045	37.0	36.0	37.0	35.0	37.0
8	36.2145638946714	37.0	36.0	37.0	35.0	37.0
9	38.05037576512642	39.0	38.0	39.0	35.0	39.0
10	37.98733944663916	39.0	38.0	39.0	35.0	39.0
11	38.053273846820204	39.0	38.0	39.0	35.0	39.0
12	37.9766401475564	39.0	38.0	39.0	35.0	39.0
13	38.04071305949584	39.0	38.0	39.0	35.0	39.0
14	39.37844542615306	40.0	39.0	41.0	37.0	41.0
15	39.40035988772671	40.0	39.0	41.0	37.0	41.0
16	39.32311664231321	40.0	39.0	41.0	36.0	41.0
17	39.305958100698	40.0	39.0	41.0	36.0	41.0
18	39.3461297469471	40.0	39.0	41.0	36.0	41.0
19	39.36438693162255	40.0	39.0	41.0	37.0	41.0
20	39.377749497215675	40.0	39.0	41.0	37.0	41.0
21	39.33306161282748	40.0	39.0	41.0	36.0	41.0
22	39.30802763762542	40.0	39.0	41.0	36.0	41.0
23	39.25401771212145	40.0	39.0	41.0	36.0	41.0
24	39.26602491960926	40.0	39.0	41.0	36.0	41.0
25	39.21002210669369	40.0	39.0	41.0	36.0	41.0
26	39.13805064464676	40.0	39.0	41.0	36.0	41.0
27	39.0054469822599	40.0	39.0	41.0	35.0	41.0
28	39.01302798437323	40.0	39.0	41.0	35.0	41.0
29	39.0007275620709	40.0	39.0	41.0	35.0	41.0
30	38.966018714648456	40.0	38.0	41.0	35.0	41.0
31	38.87312193477978	40.0	38.0	41.0	35.0	41.0
32	38.82965070937302	40.0	38.0	41.0	35.0	41.0
33	38.77637199591689	40.0	38.0	41.0	35.0	41.0
34	38.74818565729393	40.0	38.0	41.0	35.0	41.0
35	38.678836095352	40.0	38.0	41.0	35.0	41.0
36	38.624348022868325	40.0	38.0	41.0	35.0	41.0
37	38.57819284774851	40.0	38.0	41.0	35.0	41.0
38	38.53994473934906	40.0	38.0	41.0	35.0	41.0
39	38.463093258127586	40.0	38.0	41.0	34.0	41.0
40	38.15764372088383	40.0	37.0	41.0	34.0	41.0
41	38.22446993209826	40.0	37.0	41.0	34.0	41.0
42	38.20059299958755	40.0	37.0	41.0	34.0	41.0
43	37.73481822506488	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	1.0
14	1.0
15	2.0
16	1.0
17	1.0
18	5.0
19	18.0
20	16.0
21	57.0
22	96.0
23	206.0
24	342.0
25	603.0
26	1086.0
27	1619.0
28	2574.0
29	3848.0
30	5702.0
31	7749.0
32	10645.0
33	14738.0
34	21411.0
35	32242.0
36	48849.0
37	88659.0
38	202842.0
39	378605.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.7627648818685	16.67102636135015	12.860085433793678	27.706123322987676
2	20.94429770185285	19.752093565942307	32.78725622716483	26.516352505040008
3	21.016567245350235	20.02389518239543	30.070213389818758	28.889324182435583
4	14.607329397035976	16.75680081954149	36.467892977809356	32.16797680561318
5	16.024250446818012	33.07742939423766	34.65361110468986	16.244709054254475
6	31.24830428154462	35.811627123222	16.498260785986034	16.44180780924734
7	28.66509393215666	28.561191255141903	20.660937825076072	22.112776987625363
8	26.338355296055717	30.675379567185736	21.317447011459713	21.66881812529884
9	28.20848181642319	12.666636655745126	18.285897827412057	40.838983700419625
10	19.4722620002117	22.91966522411467	29.68891246503626	27.91916031063737
11	38.80898818989127	19.773871761710037	19.195228750138398	22.2219112982603
12	21.9195715413731	24.560573192379334	27.150353500267055	26.369501765980512
13	35.309998625175346	18.092814047057935	20.770923796998016	25.826263530768696
14	23.277849628249847	21.086768468579173	24.1849905648096	31.450391338361378
15	30.015220403857786	24.09142948913706	20.706319205083688	25.18703090192147
16	24.001031726816258	24.67846744768062	23.681050414698213	27.639450410804905
17	26.25148584478108	24.16102238287528	22.078467204348826	27.50902456799481
18	26.172889674580226	21.251017431073226	23.981686849011403	28.59440604533515
19	27.565112547039078	22.636913676828605	24.10700272409946	25.690971052032857
20	30.206114198045313	20.45872910236117	23.360582438987592	25.97457426060592
21	26.957634717607366	22.35586545211656	22.416820067086576	28.269679763189497
22	28.14168723834228	23.4635117888172	21.656164871891892	26.738636100948632
23	27.816960956196628	22.247826134564917	23.03634282043452	26.89887008880394
24	27.375557077731127	21.563333791608216	23.617054152274605	27.444054978386053
25	27.564017553955782	22.28408257221175	23.914162275541624	26.23773759829084
26	27.45597823640414	23.27529464438883	22.939375099613954	26.329352019593077
27	26.47936607200431	21.975659520417363	25.325608359907193	26.219366047671134
28	25.64425134714566	23.535173002823864	22.562697478960924	28.25787817106955
29	27.027349277243733	24.031691533148482	22.83462076131219	26.106338428295594
30	26.492384323105693	22.142463466772437	24.688322385430265	26.676829824691605
31	27.55610927057644	22.823062500988538	23.434433639160844	26.186394589274176
32	25.00818203165017	22.533497663406425	23.529576371509254	28.928743933434152
33	24.980198875077107	22.18200488366915	25.797672044704917	27.040124196548827
34	25.520152131039037	22.223006291343594	24.963165649336982	27.293675928280386
35	25.01487365604807	24.40216419299618	25.210269088466923	25.37269306248882
36	25.571130142361266	22.955678329965217	25.664204554441234	25.808986973232283
37	26.46890280476395	22.953001680206054	24.74465369627082	25.83344181875918
38	25.478664059772022	22.117278625856677	25.711775920615437	26.692281393755863
39	24.850746359452163	21.0473487175806	26.95678305632036	27.145121866646875
40	24.713263894549733	22.221789632362153	27.604897295732084	25.46004917735603
41	22.848125676006145	21.18677783685333	28.506076603282786	27.45901988385773
42	21.107938334856186	21.82284715234882	29.862286369891095	27.206928142903898
43	20.564091770153652	22.497727889352166	29.104064492659287	27.834115847834894
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	4.0
2	4.0
3	26.0
4	48.0
5	48.0
6	83.0
7	118.0
8	122.5
9	127.0
10	182.5
11	238.0
12	238.0
13	490.5
14	743.0
15	1462.5
16	2182.0
17	2136.0
18	2090.0
19	2090.0
20	2416.0
21	2742.0
22	2231.5
23	1721.0
24	1860.5
25	2000.0
26	2000.0
27	2224.0
28	2448.0
29	3134.0
30	3820.0
31	4712.5
32	5605.0
33	5605.0
34	7431.5
35	9258.0
36	10564.5
37	11871.0
38	15269.5
39	18668.0
40	18668.0
41	22645.0
42	26622.0
43	32466.0
44	38310.0
45	53718.5
46	69127.0
47	69127.0
48	77704.5
49	86282.0
50	95551.5
51	104821.0
52	104552.5
53	104284.0
54	104284.0
55	93331.0
56	82378.0
57	76846.0
58	71314.0
59	64524.5
60	57735.0
61	57735.0
62	50750.0
63	43765.0
64	34457.5
65	25150.0
66	21425.0
67	17700.0
68	17700.0
69	14948.5
70	12197.0
71	10017.0
72	7837.0
73	6006.0
74	4175.0
75	4175.0
76	3344.0
77	2513.0
78	2035.0
79	1557.0
80	1308.5
81	1060.0
82	1060.0
83	840.5
84	621.0
85	494.5
86	368.0
87	300.0
88	232.0
89	232.0
90	185.5
91	139.0
92	89.5
93	40.0
94	24.5
95	9.0
96	9.0
97	4.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	821923.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.5526887124563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.31777018533185	28.788529774297437
2	6.988863849208784	4.829680740709005
3	2.58053787187615	2.6749356539292313
4	1.3711788797696607	1.8951166800710253
5	0.8924889429329514	1.5418946312235724
6	0.6698704346245805	1.3887494763156565
7	0.49622590719163634	1.200215751157419
8	0.40107561604046027	1.1086592728962135
9	0.3219182438028826	1.0010826782083455
>10	2.3105703577797905	16.14579954481768
>50	0.3419727846823068	8.305611617895693
>100	0.2677543911966547	18.242406534545935
>500	0.027343618199127867	6.540929870344342
>1k	0.012428917363239938	6.336387773588433
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2634	0.3204679757106201	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2538	0.3087880494888207	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2455	0.29868977994289003	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2405	0.29260648503570286	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	2099	0.2553767202037174	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1985	0.24150680781533063	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1906	0.2318952018619749	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1813	0.22058027333460678	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1733	0.21084700148310728	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1709	0.20792701992765747	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1687	0.2052503701684951	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1679	0.20427704298334515	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1645	0.2001404024464579	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1405	0.17094058689195946	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1389	0.16899393252165956	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1364	0.165952285068066	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1317	0.16023398785531004	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1288	0.1567056768091415	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1255	0.15269070217039796	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	1152	0.1401591146615924	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	1135	0.13809079439314875	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	1133	0.13784746259686126	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1121	0.13638747181913635	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1118	0.1360224741247051	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	1094	0.13310249256925527	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	1081	0.13152083589338662	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	1079	0.13127750409709912	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	1071	0.13030417691194918	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1071	0.13030417691194918	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	1067	0.1298175133193742	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	1063	0.1293308497267992	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	1062	0.12920918382865548	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	1047	0.12738419535649934	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	1032	0.1255592068843432	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	1009	0.12276089122703708	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	1000	0.1216658981437434	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	991	0.1205709050604497	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	951	0.11570426913469997	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	947	0.11521760554212498	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	932	0.11339261706996885	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	925	0.11254095578296264	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	924	0.1124192898848189	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	924	0.1124192898848189	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	907	0.11035096961637526	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	905	0.11010763782008776	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	876	0.10657932677391921	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	863	0.10499767009805054	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	862	0.1048760041999068	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	854	0.10390267701475685	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	852	0.10365934521846937	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	843	0.10256435213517569	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	830	0.100982695459307	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	826	0.10049603186673205	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.2166589814374339E-4	0.0	0.0	0.0	1.2166589814374339E-4
7	1.2166589814374339E-4	0.0	0.0	0.0	1.2166589814374339E-4
8	1.2166589814374339E-4	0.0	0.0	0.0	1.2166589814374339E-4
9	1.2166589814374339E-4	0.0	0.0	0.0	1.2166589814374339E-4
10	1.2166589814374339E-4	0.0	0.0	0.0	1.2166589814374339E-4
11	1.2166589814374339E-4	0.0	0.0	0.0	1.2166589814374339E-4
12	1.2166589814374339E-4	0.0	0.0	0.0	1.2166589814374339E-4
13	1.2166589814374339E-4	0.0	0.0	0.0	1.2166589814374339E-4
14	1.2166589814374339E-4	0.0	0.0	0.0	1.2166589814374339E-4
15	1.2166589814374339E-4	0.0	0.0	1.2166589814374339E-4	1.2166589814374339E-4
16	1.2166589814374339E-4	0.0	0.0	0.0010949930832936906	1.2166589814374339E-4
17	1.2166589814374339E-4	0.0	0.0	0.0014599907777249207	1.2166589814374339E-4
18	1.2166589814374339E-4	0.0	0.0	0.0020683202684436378	1.2166589814374339E-4
19	1.2166589814374339E-4	0.0	0.0	0.002798315657306098	1.2166589814374339E-4
20	3.6499769443123017E-4	0.0	0.0	0.0036499769443123016	1.2166589814374339E-4
21	4.8666359257497355E-4	0.0	0.0	0.007299953888624603	1.2166589814374339E-4
22	4.8666359257497355E-4	0.0	0.0	0.011193262629224392	1.2166589814374339E-4
23	4.8666359257497355E-4	0.0	0.0	0.013748246490243003	1.2166589814374339E-4
24	4.8666359257497355E-4	0.0	0.0	0.017033225740124076	1.2166589814374339E-4
25	4.8666359257497355E-4	0.0	0.0	0.018493216517848996	1.2166589814374339E-4
26	4.8666359257497355E-4	0.0	0.0	0.021048200378867607	1.2166589814374339E-4
27	6.08329490718717E-4	0.0	0.0	0.03649976944312302	1.2166589814374339E-4
28	6.08329490718717E-4	0.0	0.0	0.11485260784769376	1.2166589814374339E-4
29	6.08329490718717E-4	0.0	0.0	0.26267667409234197	1.2166589814374339E-4
30	6.08329490718717E-4	0.0	0.0	0.46026209267778123	1.2166589814374339E-4
31	6.08329490718717E-4	0.0	0.0	0.9915770698715086	1.2166589814374339E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTTA	25	0.005495444	29.599998	8
GGTATCA	1610	0.0	28.841616	1
TAGCGAA	150	0.0	25.9	10
TAAGGTA	150	0.0	25.9	5
CGTTATT	80	9.786163E-10	25.437502	10
TGTTACG	80	9.786163E-10	25.437502	16
TCCTAAG	175	0.0	24.314287	2
TTATTGT	85	1.9754225E-9	23.941175	12
GTAGCGA	165	0.0	23.545454	9
TCCATTC	40	0.0019307215	23.125002	3
GTCTAAA	40	0.0019307215	23.125002	2
GTTACTC	40	0.0019307215	23.125002	28
GTACTAT	40	0.0019307215	23.125002	1
ATACGGC	185	0.0	23.0	29
TCGGGTA	170	0.0	22.85294	25
TAATACT	115	5.456968E-12	22.52174	4
GACCGTT	150	0.0	22.2	7
CGGGACG	60	3.7246245E-5	21.583332	16
ATTGTTA	95	7.1377144E-9	21.421053	14
GATGCTA	95	7.1377144E-9	21.421053	14
>>END_MODULE