FastQCFastQC Report
Fri 10 Feb 2017
ERR1631965.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631965.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255008
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5470.21450307441335173No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5160.2023465930480612No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4990.19568013552516No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4690.18391579872004016No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4540.17803363031748023No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4470.17528861839628562No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4380.17175931735474967No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4110.1611714142301418No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4110.1611714142301418No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4000.1568578240682645No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3730.14626992094365665No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3690.14470134270297402No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.1368584514995608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3070.12038837997239303No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3070.12038837997239303No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2830.11097691052829717No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2780.10901618772744386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2760.10823189860710253No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2620.10274187476471326No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2610.10234973020454259No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTAA250.00548842629.59999834
TGAACTA250.00548842629.59999833
AGGGGTC250.00548842629.5999987
CGGAGAC250.00548842629.59999810
GGTATCA3550.029.18311
TAGGACC451.3192713E-424.6666664
CTCTAGA451.3192713E-424.6666661
AGGACCG551.8951416E-523.5454545
CCTTGCC551.8951416E-523.5454549
GACCGTT400.001927090723.1250027
TAAACCA400.001927090723.1250025
CGCCGTT400.001927090723.12500225
GGACCGT502.6941203E-422.1999996
AGTACCT705.076279E-621.14285737
GCCTTAG450.003817770920.5555552
GGGTCGA450.003817770920.5555559
GTTACGG450.003817770920.55555517
AGAGAAT555.128024E-420.1818188
CTTATAC3500.020.08571437
GTATCAA5300.019.1981142