##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631965.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	255008
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5875501945037	34.0	31.0	34.0	31.0	34.0
2	32.755619431547245	34.0	31.0	34.0	31.0	34.0
3	32.8405226502698	34.0	31.0	34.0	31.0	34.0
4	36.28159508721295	37.0	37.0	37.0	35.0	37.0
5	36.20283285230267	37.0	35.0	37.0	35.0	37.0
6	36.247243223742004	37.0	37.0	37.0	35.0	37.0
7	36.24154536328272	37.0	37.0	37.0	35.0	37.0
8	36.22136952566194	37.0	36.0	37.0	35.0	37.0
9	38.06174316099887	39.0	38.0	39.0	35.0	39.0
10	37.99336099259631	39.0	38.0	39.0	35.0	39.0
11	38.061566695946794	39.0	38.0	39.0	35.0	39.0
12	37.99543935876522	39.0	38.0	39.0	35.0	39.0
13	38.04528485380851	39.0	38.0	39.0	35.0	39.0
14	39.42633956581754	40.0	39.0	41.0	37.0	41.0
15	39.42975906638223	40.0	39.0	41.0	37.0	41.0
16	39.370960911030245	40.0	39.0	41.0	36.0	41.0
17	39.34102851675242	40.0	39.0	41.0	36.0	41.0
18	39.37150991341448	40.0	39.0	41.0	37.0	41.0
19	39.397387532940144	40.0	39.0	41.0	37.0	41.0
20	39.40179523779646	40.0	39.0	41.0	37.0	41.0
21	39.364392489647386	40.0	39.0	41.0	36.0	41.0
22	39.339557974651775	40.0	39.0	41.0	36.0	41.0
23	39.28305778642239	40.0	39.0	41.0	36.0	41.0
24	39.29461820805622	40.0	39.0	41.0	36.0	41.0
25	39.241663006650775	40.0	39.0	41.0	36.0	41.0
26	39.1817550821935	40.0	39.0	41.0	36.0	41.0
27	39.067335142426906	40.0	39.0	41.0	35.0	41.0
28	39.0698566319488	40.0	39.0	41.0	35.0	41.0
29	39.05066507717405	40.0	39.0	41.0	35.0	41.0
30	39.01045457397415	40.0	39.0	41.0	35.0	41.0
31	38.91475953695571	40.0	38.0	41.0	35.0	41.0
32	38.866694378215584	40.0	38.0	41.0	35.0	41.0
33	38.8223859643619	40.0	38.0	41.0	35.0	41.0
34	38.78808115823817	40.0	38.0	41.0	35.0	41.0
35	38.7235733780901	40.0	38.0	41.0	35.0	41.0
36	38.673994541347724	40.0	38.0	41.0	35.0	41.0
37	38.61934919688794	40.0	38.0	41.0	35.0	41.0
38	38.583358169155474	40.0	38.0	41.0	35.0	41.0
39	38.51875235286736	40.0	38.0	41.0	35.0	41.0
40	38.21684025599197	40.0	38.0	41.0	34.0	41.0
41	38.29282610741624	40.0	38.0	41.0	34.0	41.0
42	38.275995262893716	40.0	37.0	41.0	34.0	41.0
43	37.81033144058225	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	4.0
18	1.0
19	3.0
20	6.0
21	17.0
22	42.0
23	66.0
24	99.0
25	172.0
26	321.0
27	481.0
28	821.0
29	1167.0
30	1658.0
31	2347.0
32	3194.0
33	4395.0
34	6597.0
35	9831.0
36	15015.0
37	26513.0
38	61246.0
39	121011.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.666504580248464	16.471247960848288	12.974494917806501	27.88775254109675
2	20.41700652528548	19.315864600326265	33.22405571589911	27.043073158489143
3	20.663273309072657	19.995451123102022	29.669657422512234	29.67161814531309
4	15.256384113439578	16.04537896850295	34.2534351863471	34.44480173171038
5	16.631242941397918	33.07504078303426	33.688747019701346	16.604969255866482
6	33.691884176182704	33.98246329526916	15.778328523026728	16.547324005521396
7	30.05748839252102	28.265779897101268	20.117408081315098	21.559323629062618
8	27.101110553394403	31.357839754046935	20.356616263019202	21.184433429539464
9	28.203428912034134	13.118411971389133	18.011591793198644	40.66656732337809
10	19.188025473710628	23.911014556406073	29.713577613251346	27.18738235663195
11	38.727020328773996	19.690362655289245	19.027638348600828	22.554978667335927
12	22.712620780524535	24.254925335675743	26.672104404567698	26.360349479232028
13	34.57107228008533	17.918653532438196	21.332271928723806	26.178002258752663
14	23.737294516250472	20.360537708620907	23.551025850169406	32.351141924959215
15	29.373588279583384	24.54354373196135	20.527199146693437	25.555668841761825
16	25.369400175680763	24.36002007780148	22.660073409461663	27.61050633705609
17	26.574068264525035	24.171398544359395	21.98793763332915	27.26659555778642
18	26.283489145438576	21.74520015058351	23.869055088467814	28.102255615510103
19	28.00931735474966	22.6408583260133	23.696903626552892	25.65292069268415
20	29.5418182958966	20.87895281716652	23.543967248086332	26.035261638850542
21	27.148952189735226	22.631446856569205	22.24283159744008	27.976769356255488
22	28.17205734722048	23.16241059104028	21.676574852553646	26.988957209185592
23	27.365415986949433	22.66517128874388	22.867910026352114	27.101502697954572
24	27.63128999874514	21.992643368051198	23.242800225875264	27.1332664073284
25	27.486980800602336	22.362827832852304	23.52161500815661	26.628576358388756
26	27.439531308821685	23.004768477851677	23.080452377964615	26.475247835362026
27	26.516030869619776	22.031465679508095	24.670990714016817	26.781512736855312
28	25.947421257372316	23.252603839879534	22.721247960848288	28.078726941899863
29	26.984251474463544	23.71258940895972	22.766344585267912	26.53681453130882
30	26.42152403061865	22.78869682519764	23.84238925837621	26.947389885807503
31	27.465412849792948	22.633799723930228	23.448676119964865	26.45211130631196
32	24.842357886811396	22.737325887815285	23.703570084075793	28.716746141297527
33	25.525865855188854	22.109502446982056	25.22430668841762	27.14032500941147
34	25.704291630066507	22.13538398795332	24.90117957083699	27.259144811143184
35	25.295284853808507	23.603573221232274	25.12862341573598	25.972518509223242
36	25.57958965993224	22.4804712009035	25.458024846279333	26.481914292884927
37	25.833699334922827	22.101267411218473	25.2552861086711	26.8097471451876
38	25.241168904504956	21.222863596436188	26.469365666959465	27.066601832099384
39	24.552563056845276	20.164073283975405	27.710111055339443	27.573252603839883
40	24.118851173296523	21.297371062868617	28.455185719663696	26.12859204417116
41	22.55027293261388	20.553472832224873	29.497898105157482	27.398356130003766
42	21.421288743882545	21.101298782783285	30.14650520767976	27.330907265654407
43	20.44641736729828	21.546382858576983	30.094742125737234	27.9124576483875
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	3.0
2	5.0
3	9.5
4	14.0
5	14.0
6	22.0
7	30.0
8	24.5
9	19.0
10	45.0
11	71.0
12	71.0
13	153.5
14	236.0
15	438.5
16	641.0
17	633.5
18	626.0
19	626.0
20	690.0
21	754.0
22	635.5
23	517.0
24	564.0
25	611.0
26	611.0
27	727.0
28	843.0
29	1247.5
30	1652.0
31	1857.5
32	2063.0
33	2063.0
34	2646.0
35	3229.0
36	3692.5
37	4156.0
38	5438.5
39	6721.0
40	6721.0
41	7915.5
42	9110.0
43	10319.0
44	11528.0
45	15671.5
46	19815.0
47	19815.0
48	22300.0
49	24785.0
50	27571.5
51	30358.0
52	30741.5
53	31125.0
54	31125.0
55	27737.5
56	24350.0
57	22803.5
58	21257.0
59	19298.0
60	17339.0
61	17339.0
62	15663.0
63	13987.0
64	11524.5
65	9062.0
66	7883.0
67	6704.0
68	6704.0
69	5718.5
70	4733.0
71	4095.0
72	3457.0
73	2752.5
74	2048.0
75	2048.0
76	1671.5
77	1295.0
78	1057.0
79	819.0
80	644.5
81	470.0
82	470.0
83	364.5
84	259.0
85	219.5
86	180.0
87	126.5
88	73.0
89	73.0
90	61.0
91	49.0
92	29.5
93	10.0
94	8.0
95	6.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	255008.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.423980969219606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.34746752611656	37.76473284326645
2	8.438040294233094	7.834548440739707
3	2.938373639357042	4.092330057419035
4	1.5635381723531523	2.903426654318846
5	1.0472536762976294	2.430884236919321
6	0.7277576588465381	2.0271244622697298
7	0.4973992767990822	1.6163878192156929
8	0.38535414544857616	1.4311738811771666
9	0.3367911870583077	1.4071668892736182
>10	2.382233963930159	21.417737102574545
>50	0.2238603063732401	7.079766537046335
>100	0.10994908852844978	9.505906329814357
>500	0.0019810646581702664	0.4888147459652023
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	547	0.21450307441335173	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	516	0.2023465930480612	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	499	0.19568013552516	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	469	0.18391579872004016	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	454	0.17803363031748023	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	447	0.17528861839628562	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	438	0.17175931735474967	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	411	0.1611714142301418	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	411	0.1611714142301418	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	400	0.1568578240682645	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	373	0.14626992094365665	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	369	0.14470134270297402	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	349	0.1368584514995608	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	307	0.12038837997239303	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	307	0.12038837997239303	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	283	0.11097691052829717	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	278	0.10901618772744386	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	276	0.10823189860710253	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	262	0.10274187476471326	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	261	0.10234973020454259	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	3.921445601706613E-4	0.0
16	0.0	0.0	0.0	0.001176433680511984	0.0
17	0.0	0.0	0.0	0.002352867361023968	0.0
18	0.0	0.0	0.0	0.002745011921194629	0.0
19	0.0	0.0	0.0	0.006274312962730581	0.0
20	0.0	0.0	0.0	0.007058602083071903	0.0
21	0.0	0.0	0.0	0.010587903124607856	0.0
22	0.0	0.0	0.0	0.012940770485631824	0.0
23	0.0	0.0	0.0	0.016077926966997114	0.0
24	0.0	0.0	0.0	0.020391517128874388	0.0
25	0.0	0.0	0.0	0.022352239929727694	0.0
26	0.0	0.0	0.0	0.028234408332287613	0.0
27	0.0	0.0	0.0	0.05058664826201531	0.0
28	0.0	0.0	0.0	0.1650928598318484	0.0
29	0.0	0.0	0.0	0.36351800727820305	0.0
30	0.0	0.0	0.0	0.5988047433805999	0.0
31	0.0	0.0	0.0	1.32231145689547	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACTAA	25	0.005488426	29.599998	34
TGAACTA	25	0.005488426	29.599998	33
AGGGGTC	25	0.005488426	29.599998	7
CGGAGAC	25	0.005488426	29.599998	10
GGTATCA	355	0.0	29.1831	1
TAGGACC	45	1.3192713E-4	24.666666	4
CTCTAGA	45	1.3192713E-4	24.666666	1
AGGACCG	55	1.8951416E-5	23.545454	5
CCTTGCC	55	1.8951416E-5	23.545454	9
GACCGTT	40	0.0019270907	23.125002	7
TAAACCA	40	0.0019270907	23.125002	5
CGCCGTT	40	0.0019270907	23.125002	25
GGACCGT	50	2.6941203E-4	22.199999	6
AGTACCT	70	5.076279E-6	21.142857	37
GCCTTAG	45	0.0038177709	20.555555	2
GGGTCGA	45	0.0038177709	20.555555	9
GTTACGG	45	0.0038177709	20.555555	17
AGAGAAT	55	5.128024E-4	20.181818	8
CTTATAC	350	0.0	20.085714	37
GTATCAA	530	0.0	19.198114	2
>>END_MODULE