FastQCFastQC Report
Fri 10 Feb 2017
ERR1631964.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631964.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580605
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16070.2767802550787541No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15460.2662739728386769No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT15410.26541280216326074No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA14310.2464670473041052No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13740.23664970160436094No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA13070.2251100145537844No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA11640.20048053323688222No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA10430.17964020289181112No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10080.173612008163898No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9790.16861721824648426No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC9420.16224455524840467No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC9210.1586276384116568No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9160.15776646773624065No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8840.15225497541357721No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA8770.1510493364679946No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC8670.14932699511716227No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC8630.14863805857682935No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC8420.14502114174008146No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7900.13606496671575338No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7780.1339981570947546No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7660.13193134747375582No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7530.1296923037176738No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7500.12917560131242412No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7320.12607538688092593No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7170.12349187485467744No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT6950.11970272388284633No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6920.11918602147759665No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT6720.115741338775932No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT6640.11436346569526615No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6270.10799080269718656No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6240.10747410029193687No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA6210.10695739788668715No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC6210.10695739788668715No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6170.10626846134635423No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6130.1055795248060213No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6080.10471835413060514No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC6050.10420165172535545No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5920.10196260796927342No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA5880.10127367142894049No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5860.10092920315877404No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5840.10058473488860757No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC5810.10006803248335788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6950.032.2086331
CCGCTTA303.597304E-430.83333425
GCTTATT303.597304E-430.83333427
TCAGAAC250.00549413229.5999983
ACTGTGC900.028.7777798
CTGTGCA950.027.2631599
TGTAGAT358.864089E-426.428573
CTTATTG901.4188117E-1024.66666828
TATTAGA851.9717845E-923.9411752
TTTTACG701.9195068E-723.7857134
CAGTCGG1103.6379788E-1223.54545410
ACGAAAG1103.6379788E-1223.54545419
GACCGTT802.7199349E-823.1257
AACCAAC400.001930042423.12511
TAGATAT400.001930042423.1255
ACACGAA1054.0017767E-1122.9047617
CACGAAA1054.0017767E-1122.9047618
ATACGGC1300.022.7692329
AGCCGCG6250.022.219
CGTTATT502.6998718E-422.19999910
Produced by FastQC (version 0.11.5)