##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631964.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	580605
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20443675131975	33.0	31.0	34.0	30.0	34.0
2	32.378052204166345	34.0	31.0	34.0	31.0	34.0
3	32.470712446499775	34.0	31.0	34.0	30.0	34.0
4	36.01647419502071	37.0	35.0	37.0	35.0	37.0
5	35.88730031604964	37.0	35.0	37.0	35.0	37.0
6	35.93468537129374	37.0	35.0	37.0	35.0	37.0
7	35.939914399634866	37.0	35.0	37.0	35.0	37.0
8	35.91444097105605	37.0	35.0	37.0	35.0	37.0
9	37.71590323886291	39.0	37.0	39.0	35.0	39.0
10	37.6065259513783	39.0	37.0	39.0	35.0	39.0
11	37.675791631143376	39.0	37.0	39.0	35.0	39.0
12	37.593637671050026	39.0	37.0	39.0	35.0	39.0
13	37.66274834009352	39.0	37.0	39.0	35.0	39.0
14	38.9143204071615	40.0	38.0	41.0	36.0	41.0
15	38.91378475900139	40.0	38.0	41.0	36.0	41.0
16	38.84609846625503	40.0	38.0	41.0	35.0	41.0
17	38.82263845471534	40.0	38.0	41.0	35.0	41.0
18	38.85200265240568	40.0	38.0	41.0	36.0	41.0
19	38.86588644603474	40.0	38.0	41.0	35.0	41.0
20	38.86326332015742	40.0	38.0	41.0	35.0	41.0
21	38.8081656203443	40.0	38.0	41.0	35.0	41.0
22	38.77666916406162	40.0	38.0	41.0	35.0	41.0
23	38.72181086969626	40.0	38.0	41.0	35.0	41.0
24	38.724253149731744	40.0	38.0	41.0	35.0	41.0
25	38.66118962117102	40.0	38.0	41.0	35.0	41.0
26	38.55768896237545	40.0	38.0	41.0	35.0	41.0
27	38.41119349643906	40.0	38.0	41.0	34.0	41.0
28	38.41506015277168	40.0	38.0	41.0	34.0	41.0
29	38.40077333126652	40.0	38.0	41.0	34.0	41.0
30	38.345375944058354	40.0	38.0	41.0	34.0	41.0
31	38.27338896495897	40.0	38.0	41.0	34.0	41.0
32	38.224331516263206	40.0	38.0	41.0	34.0	41.0
33	38.1610010247931	40.0	38.0	41.0	34.0	41.0
34	38.12648874880512	40.0	38.0	41.0	33.0	41.0
35	38.05630333875871	40.0	37.0	41.0	33.0	41.0
36	37.98725639634519	40.0	37.0	41.0	33.0	41.0
37	37.93683313095823	40.0	37.0	41.0	33.0	41.0
38	37.88994238768181	40.0	37.0	41.0	33.0	41.0
39	37.80503957079254	40.0	37.0	41.0	33.0	41.0
40	37.50514032776156	40.0	37.0	41.0	33.0	41.0
41	37.576991241894234	39.0	36.0	41.0	33.0	41.0
42	37.54666425538878	39.0	36.0	41.0	33.0	41.0
43	37.017702224403855	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	4.0
18	6.0
19	18.0
20	37.0
21	59.0
22	142.0
23	267.0
24	448.0
25	783.0
26	1270.0
27	1987.0
28	2794.0
29	4051.0
30	5634.0
31	8089.0
32	10650.0
33	14699.0
34	20850.0
35	29631.0
36	46084.0
37	80830.0
38	173062.0
39	179208.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.52701061823443	16.792483702344967	12.29407256224111	27.386433117179493
2	21.85961195649366	20.039269382798977	32.02969316488835	26.071425495819017
3	21.935739444200447	20.0533925818758	29.171639927317194	28.839228046606557
4	15.742716649012667	16.086840450908966	35.33107706616374	32.83936583391463
5	17.2204855280268	33.300781081802604	32.81955890838005	16.659174481790544
6	32.94925121209772	34.90858673280457	15.719120572506265	16.423041482591437
7	29.607564523212854	27.879883914192956	19.76731168350255	22.745239879091635
8	26.722642760568714	31.069315627664245	20.72355560148466	21.484486010282378
9	27.92277021382868	12.624934335735999	17.380663273654207	42.071632176781115
10	19.79900276435787	22.779170003703033	29.610148035239103	27.811679196699995
11	39.546507522325854	19.623840648978224	19.37702913340395	21.452622695291982
12	22.788642881132613	23.168763617261305	27.397800570094986	26.6447929315111
13	35.052574469734154	17.947658046348206	21.004469475805408	25.99529800811223
14	24.079882191851603	19.68928962030985	23.726113278390645	32.5047149094479
15	29.983207171829385	23.62501184109679	20.171545198542898	26.22023578853093
16	24.982561293822823	23.594526399187053	22.70803730591366	28.71487500107646
17	26.40986557125757	23.313784759001386	22.065087279647955	28.211262390093093
18	26.28516805745731	21.299506549202988	23.616916836747876	28.79840855659183
19	28.460829651828696	22.081105054210695	23.22904556454044	26.229019729420173
20	29.858337423894042	20.384771057775943	23.02942620197897	26.727465316351047
21	27.76569268263277	21.70942379070108	22.099189638394435	28.42569388827172
22	27.953944592278745	23.39697384624659	21.01411458737007	27.634966974104596
23	28.371784603990662	22.046658227194047	22.02168427760698	27.559872891208308
24	28.028694206904863	21.03013236193281	22.467426219202384	28.47374721195994
25	28.58466599495354	22.27865760715116	22.849097062546825	26.28757933534847
26	28.508194038976587	22.608830444105717	22.23749364886627	26.64548186805143
27	27.999242169805633	21.622273318348963	23.89645283798796	26.482031673857442
28	26.597773012633375	23.416608537646074	21.546145830642175	28.439472619078376
29	27.712644569027134	23.249369192480255	21.455206207318227	27.58278003117438
30	26.87128081914555	23.208549702465533	23.62570077763712	26.2944687007518
31	28.424316015191053	22.081449522480863	22.469837497093547	27.024396965234537
32	25.188897787652536	22.33945625683554	22.8596033447869	29.61204261072502
33	25.927437758889432	22.146209557272158	24.46069186452063	27.465660819317783
34	25.541116593897744	22.16618871694181	24.008577259927144	28.2841174292333
35	26.310658709449626	23.670137184488592	24.12225178908208	25.896952316979704
36	26.231086539041172	22.920918696876534	24.633787170279277	26.214207593803017
37	27.486156681392686	22.316204648599307	23.58195330732598	26.61568536268203
38	25.97893576527932	21.77194478173629	24.80860481738876	27.44051463559563
39	25.257619207550746	20.642950026265705	26.143763832553972	27.955666933629576
40	25.16960756452321	22.129158377898918	26.560742673590475	26.140491383987392
41	24.167377132473884	20.49810111866071	27.716433719998967	27.61808802886644
42	22.7230216756659	20.92128038856021	29.135470758949715	27.220227176824174
43	21.961746798598014	21.627095874131292	28.596722384409368	27.814434942861325
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	4.0
2	5.0
3	8.5
4	12.0
5	12.0
6	24.5
7	37.0
8	49.0
9	61.0
10	68.5
11	76.0
12	76.0
13	183.0
14	290.0
15	573.5
16	857.0
17	816.0
18	775.0
19	775.0
20	910.5
21	1046.0
22	848.5
23	651.0
24	771.5
25	892.0
26	892.0
27	1040.5
28	1189.0
29	1601.5
30	2014.0
31	2557.5
32	3101.0
33	3101.0
34	4130.0
35	5159.0
36	5803.5
37	6448.0
38	8603.5
39	10759.0
40	10759.0
41	13073.5
42	15388.0
43	19137.5
44	22887.0
45	32269.5
46	41652.0
47	41652.0
48	49535.5
49	57419.0
50	62609.0
51	67799.0
52	70718.5
53	73638.0
54	73638.0
55	70658.5
56	67679.0
57	64564.5
58	61450.0
59	54306.0
60	47162.0
61	47162.0
62	40620.0
63	34078.0
64	27261.0
65	20444.0
66	17366.5
67	14289.0
68	14289.0
69	11826.5
70	9364.0
71	7854.5
72	6345.0
73	4735.5
74	3126.0
75	3126.0
76	2516.5
77	1907.0
78	1538.5
79	1170.0
80	901.0
81	632.0
82	632.0
83	498.0
84	364.0
85	290.5
86	217.0
87	171.0
88	125.0
89	125.0
90	99.5
91	74.0
92	46.0
93	18.0
94	10.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	580605.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.469693314498606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.78900053083423	32.61806846864053
2	6.424830863761686	4.943225458528745
3	2.290261612947328	2.643169855801579
4	1.2630615971236203	1.9435836911466584
5	0.854409450094596	1.6434434755074254
6	0.5939203916149306	1.3708761191191976
7	0.4402344953147665	1.1854980214855497
8	0.3291308542638402	1.0129250419095097
9	0.27515153926978353	0.9526495797648712
>10	2.1840199341360553	17.41691169848242
>50	0.29217019934004856	7.980705109219639
>100	0.23634723367242857	18.306852892231056
>500	0.023409630763745304	5.90023528418572
>1k	0.004051666862955918	2.0818553039771213
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1607	0.2767802550787541	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1546	0.2662739728386769	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1541	0.26541280216326074	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1431	0.2464670473041052	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1374	0.23664970160436094	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1307	0.2251100145537844	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	1164	0.20048053323688222	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	1043	0.17964020289181112	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1008	0.173612008163898	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	979	0.16861721824648426	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	942	0.16224455524840467	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	921	0.1586276384116568	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	916	0.15776646773624065	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	884	0.15225497541357721	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	877	0.1510493364679946	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	867	0.14932699511716227	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	863	0.14863805857682935	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	842	0.14502114174008146	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	790	0.13606496671575338	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	778	0.1339981570947546	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	766	0.13193134747375582	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	753	0.1296923037176738	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	750	0.12917560131242412	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	732	0.12607538688092593	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	717	0.12349187485467744	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	695	0.11970272388284633	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	692	0.11918602147759665	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	672	0.115741338775932	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	664	0.11436346569526615	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	627	0.10799080269718656	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	624	0.10747410029193687	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	621	0.10695739788668715	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	621	0.10695739788668715	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	617	0.10626846134635423	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	613	0.1055795248060213	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	608	0.10471835413060514	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	605	0.10420165172535545	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	592	0.10196260796927342	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	588	0.10127367142894049	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	586	0.10092920315877404	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	584	0.10058473488860757	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	581	0.10006803248335788	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	1.7223413508323215E-4	0.0	0.0	0.0	0.0
13	1.7223413508323215E-4	0.0	0.0	0.0	0.0
14	1.7223413508323215E-4	0.0	0.0	3.444682701664643E-4	0.0
15	1.7223413508323215E-4	0.0	0.0	5.167024052496964E-4	0.0
16	1.7223413508323215E-4	0.0	0.0	0.0010334048104993928	0.0
17	1.7223413508323215E-4	0.0	0.0	0.001205638945582625	0.0
18	1.7223413508323215E-4	0.0	0.0	0.0015501072157490894	0.0
19	3.444682701664643E-4	0.0	0.0	0.0018945754859155536	0.0
20	3.444682701664643E-4	0.0	0.0	0.0029279802964149466	0.0
21	3.444682701664643E-4	0.0	0.0	0.005167024052496964	0.0
22	3.444682701664643E-4	0.0	0.0	0.007406067808578982	0.0
23	3.444682701664643E-4	0.0	0.0	0.009817345699744233	0.0
24	3.444682701664643E-4	0.0	0.0	0.011195218780410089	0.0
25	3.444682701664643E-4	0.0	0.0	0.01291756013124241	0.0
26	3.444682701664643E-4	0.0	0.0	0.016190008697823822	0.0
27	3.444682701664643E-4	0.0	0.0	0.028935334693983	0.0
28	3.444682701664643E-4	0.0	0.0	0.0926619646747789	0.0
29	3.444682701664643E-4	0.0	0.0	0.19720808467030082	0.0
30	3.444682701664643E-4	0.0	0.0	0.35118540143471033	0.0
31	3.444682701664643E-4	0.0	0.0	0.7481850828015605	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	695	0.0	32.208633	1
CCGCTTA	30	3.597304E-4	30.833334	25
GCTTATT	30	3.597304E-4	30.833334	27
TCAGAAC	25	0.005494132	29.599998	3
ACTGTGC	90	0.0	28.777779	8
CTGTGCA	95	0.0	27.263159	9
TGTAGAT	35	8.864089E-4	26.42857	3
CTTATTG	90	1.4188117E-10	24.666668	28
TATTAGA	85	1.9717845E-9	23.941175	2
TTTTACG	70	1.9195068E-7	23.785713	4
CAGTCGG	110	3.6379788E-12	23.545454	10
ACGAAAG	110	3.6379788E-12	23.545454	19
GACCGTT	80	2.7199349E-8	23.125	7
AACCAAC	40	0.0019300424	23.125	11
TAGATAT	40	0.0019300424	23.125	5
ACACGAA	105	4.0017767E-11	22.90476	17
CACGAAA	105	4.0017767E-11	22.90476	18
ATACGGC	130	0.0	22.76923	29
AGCCGCG	625	0.0	22.2	19
CGTTATT	50	2.6998718E-4	22.199999	10
>>END_MODULE