FastQCFastQC Report
Fri 10 Feb 2017
ERR1631963.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631963.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489222
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC15880.32459701321690354No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA12240.25019316383972917No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT10480.21421767622878773No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA10190.2082898970201667No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8970.18335234310803683No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8360.17088356615197192No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8190.16740866109864233No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7680.1569839459386536No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7660.1565751335794384No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7610.15555310268140027No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG7000.14308432572533533No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6870.14042704539043624No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6400.13081995494887802No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG6350.1297979240508399No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6350.1297979240508399No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT6110.12489217574025697No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT5900.12059964596849691No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5870.11998642742967407No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5760.11773795945399022No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5730.11712474091516735No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT5720.11692033473555974No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5690.11630711619673686No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC5680.11610271001712923No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5650.11548949147830638No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT5600.11446746058026826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5390.1101749308085082No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5380.10997052462890057No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5310.10853968137164723No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT5210.10649561957557102No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT5190.10608680721635577No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC5180.10588240103674815No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG5150.10526918249792527No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5050.10322512070184905No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5030.10281630834263382No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTTT200.001840902637.018
GGTATCA5750.030.8869571
GTTTAGG303.596482E-430.8333341
CTTATTG1050.028.19047528
ATACGGC905.456968E-1226.72222129
CTTTATG358.8620826E-426.4285721
ACACGAA509.077157E-625.90000217
CACGAAA509.077157E-625.90000218
AAACACG509.077157E-625.90000215
CTTTTTA451.3216947E-424.6666662
TAAACCA451.3216947E-424.6666665
TACGAGG451.3216947E-424.6666667
GACAGGC3400.023.9411757
TCACGTA851.9699655E-923.94117525
GTGATTC1250.023.6816
GGACGTA551.8997638E-523.54545427
CATCAGA3500.023.25714116
TAAAGTG400.001929610223.1250025
CCAGGAC4000.023.1250023
GAACTAA400.001929610223.12500211
Produced by FastQC (version 0.11.5)