##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631963.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	489222
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.549423370167325	34.0	31.0	34.0	31.0	34.0
2	32.71987359521853	34.0	31.0	34.0	31.0	34.0
3	32.81001467636369	34.0	31.0	34.0	31.0	34.0
4	36.25358630642121	37.0	37.0	37.0	35.0	37.0
5	36.1702437748098	37.0	35.0	37.0	35.0	37.0
6	36.21725924018135	37.0	36.0	37.0	35.0	37.0
7	36.21354517989788	37.0	36.0	37.0	35.0	37.0
8	36.189186095474035	37.0	36.0	37.0	35.0	37.0
9	38.04543540560318	39.0	38.0	39.0	35.0	39.0
10	37.97346807788693	39.0	38.0	39.0	35.0	39.0
11	38.04200546990937	39.0	38.0	39.0	35.0	39.0
12	37.97322892265679	39.0	38.0	39.0	35.0	39.0
13	38.0175564467665	39.0	38.0	39.0	35.0	39.0
14	39.392001995004314	40.0	39.0	41.0	37.0	41.0
15	39.40551324347638	40.0	39.0	41.0	37.0	41.0
16	39.35914370163239	40.0	39.0	41.0	36.0	41.0
17	39.327883864585	40.0	39.0	41.0	36.0	41.0
18	39.32082367514135	40.0	39.0	41.0	36.0	41.0
19	39.34308759622421	40.0	39.0	41.0	36.0	41.0
20	39.32639578759745	40.0	39.0	41.0	36.0	41.0
21	39.29897878672668	40.0	39.0	41.0	36.0	41.0
22	39.262396212762305	40.0	39.0	41.0	36.0	41.0
23	39.19592127909211	40.0	39.0	41.0	36.0	41.0
24	39.209144314850924	40.0	39.0	41.0	36.0	41.0
25	39.15047360911815	40.0	39.0	41.0	36.0	41.0
26	39.08077314593375	40.0	39.0	41.0	35.0	41.0
27	38.96298408493485	40.0	39.0	41.0	35.0	41.0
28	38.95871608390465	40.0	38.0	41.0	35.0	41.0
29	38.93669745023732	40.0	38.0	41.0	35.0	41.0
30	38.8907571613705	40.0	38.0	41.0	35.0	41.0
31	38.83708623079093	40.0	38.0	41.0	35.0	41.0
32	38.7883414891399	40.0	38.0	41.0	35.0	41.0
33	38.725496809219536	40.0	38.0	41.0	35.0	41.0
34	38.68323787564746	40.0	38.0	41.0	35.0	41.0
35	38.59368139617597	40.0	38.0	41.0	35.0	41.0
36	38.557237818413725	40.0	38.0	41.0	35.0	41.0
37	38.51709653286238	40.0	38.0	41.0	34.0	41.0
38	38.497224164080926	40.0	38.0	41.0	34.0	41.0
39	38.42865406706976	40.0	38.0	41.0	34.0	41.0
40	38.110213767982636	40.0	37.0	41.0	34.0	41.0
41	38.17485313415995	40.0	37.0	41.0	34.0	41.0
42	38.15929169170642	40.0	37.0	41.0	34.0	41.0
43	37.66789514780611	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	3.0
18	3.0
19	3.0
20	8.0
21	28.0
22	78.0
23	131.0
24	213.0
25	397.0
26	656.0
27	1022.0
28	1588.0
29	2361.0
30	3359.0
31	4768.0
32	6357.0
33	8889.0
34	13212.0
35	21077.0
36	30409.0
37	52509.0
38	118483.0
39	223666.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.75539121298715	15.467211204729143	11.858420103756577	28.91897747852713
2	21.10044110853559	18.26164808614494	32.90919051064752	27.728720294671948
3	21.555040451982943	19.13671094104517	28.56228869511183	30.745959911860055
4	15.62133346415329	15.034687728679414	33.54509813540683	35.79888067176047
5	17.165622151088872	32.936376532535334	32.643462477157605	17.254538839218185
6	35.2553646401838	33.16653789077351	15.08129233762995	16.496805131412735
7	30.978165331894314	27.33339874331081	19.182906737636493	22.505529187158384
8	28.196197227434578	30.803806860689015	19.50709493849418	21.492900973382227
9	29.026903941359954	12.426873689245372	16.38192885847325	42.164293510921425
10	19.89996361570003	22.71136621002326	28.5105739316711	28.878096242605604
11	40.11798324686952	19.16859830506396	18.212386196859505	22.501032251207018
12	24.357857986762657	22.698284214528375	25.976550523075414	26.967307275633555
13	34.99331591792683	16.92340082825384	20.426309528189655	27.656973725629676
14	24.04205043926888	19.125059788807537	23.0878823928605	33.74500737906308
15	30.32365674479071	22.764103004362028	19.829852296094614	27.082387954752647
16	25.81343439174853	23.187428202329414	21.09655739112305	29.902580014799007
17	27.113662100232617	23.4552002976154	21.270507049969133	28.16063055218285
18	26.894743081872853	20.29794244739607	23.14981746528161	29.657497005449468
19	28.75381728540417	21.410525283000357	22.21568122447478	27.619976207120693
20	29.679572872847093	20.234780937897316	22.351611333913844	27.73403485534175
21	29.380322225901534	20.8539272559288	21.058333435536422	28.707417082633242
22	29.571033191475443	22.447886644509037	20.17836483232561	27.80271533168991
23	29.10028575983909	21.7710977838282	21.07489033608464	28.05372612024807
24	29.176324858653125	20.776866126216728	21.62065483563699	28.426154179493157
25	28.601534681596497	21.27357314266325	22.504915968619564	27.619976207120693
26	29.379300195003495	21.669916724922427	22.04254919034712	26.908233889726958
27	29.062061804252465	20.742730294222255	22.899215489082668	27.295992412442615
28	26.990200767749613	21.836712167482247	21.836916573661856	29.33617049110629
29	28.313321968349747	22.166623741368948	21.957516219630353	27.562538070650948
30	27.291291070311637	21.060990715871323	23.779388498473086	27.868329715343954
31	27.963582995041108	21.52274427560494	22.403121691174967	28.11055103817899
32	25.701010992964342	21.40337106671409	23.010003638429996	29.88561430189157
33	26.902714922877546	21.75576732035763	23.6859748743924	27.65554288237242
34	27.098944855300864	21.81852001749717	23.349726708937865	27.7328084182641
35	27.000012264370778	22.64370776457314	24.079456770137075	26.276823200919008
36	27.06317377386953	21.602462685651915	23.641209921058334	27.693153619420226
37	26.471417883905467	21.558515357036274	23.93902972474664	28.031037034311623
38	26.838326976301147	20.33412234118662	25.03423803508428	27.79331264742796
39	25.099852418738323	19.872777593812216	26.376573416567528	28.65079657088193
40	25.297104382059675	20.18183973737894	27.717273548613925	26.803782331947463
41	23.474210072318908	19.388334948142152	28.350932705397547	28.78652227414139
42	21.747795479352934	20.37255070295285	29.40239809329916	28.47725572439506
43	21.251701681445233	19.881567059535342	29.62417879817343	29.242552460845992
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	5.0
2	2.0
3	11.0
4	20.0
5	20.0
6	30.0
7	40.0
8	34.5
9	29.0
10	63.5
11	98.0
12	98.0
13	163.5
14	229.0
15	543.0
16	857.0
17	806.5
18	756.0
19	756.0
20	812.5
21	869.0
22	695.0
23	521.0
24	572.0
25	623.0
26	623.0
27	709.5
28	796.0
29	1084.0
30	1372.0
31	1816.5
32	2261.0
33	2261.0
34	3244.5
35	4228.0
36	4953.0
37	5678.0
38	7502.5
39	9327.0
40	9327.0
41	11236.5
42	13146.0
43	16250.5
44	19355.0
45	25868.5
46	32382.0
47	32382.0
48	36668.0
49	40954.0
50	46418.0
51	51882.0
52	54442.5
53	57003.0
54	57003.0
55	54496.5
56	51990.0
57	48702.5
58	45415.0
59	41638.0
60	37861.0
61	37861.0
62	36022.0
63	34183.0
64	26946.0
65	19709.0
66	17328.0
67	14947.0
68	14947.0
69	12774.0
70	10601.0
71	9953.0
72	9305.0
73	10200.0
74	11095.0
75	11095.0
76	9408.0
77	7721.0
78	5091.0
79	2461.0
80	1576.0
81	691.0
82	691.0
83	533.5
84	376.0
85	302.0
86	228.0
87	177.0
88	126.0
89	126.0
90	92.0
91	58.0
92	37.0
93	16.0
94	9.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	489222.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.7311373199504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.97977473612251	32.52632187403262
2	6.67884437536123	5.173584772813883
3	2.4480735991050384	2.8445002420884733
4	1.3510202212142783	2.093061988395199
5	0.8754797581529574	1.6954163366929582
6	0.61202663513668	1.422269258936756
7	0.4758253127030208	1.2900478868626326
8	0.37623363969755064	1.165756541080846
9	0.3067525115118583	1.0692786281944904
>10	2.304131652565305	18.197606864262355
>50	0.3222877793428428	8.893987462398083
>100	0.2514377385947466	18.78307320422052
>500	0.015981212198818644	3.8384425441448924
>1k	0.0021308282931758187	1.0066523958763132
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1588	0.32459701321690354	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1224	0.25019316383972917	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	1048	0.21421767622878773	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1019	0.2082898970201667	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	897	0.18335234310803683	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	836	0.17088356615197192	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	819	0.16740866109864233	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	768	0.1569839459386536	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	766	0.1565751335794384	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	761	0.15555310268140027	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	700	0.14308432572533533	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	687	0.14042704539043624	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	640	0.13081995494887802	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	635	0.1297979240508399	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	635	0.1297979240508399	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	611	0.12489217574025697	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	590	0.12059964596849691	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	587	0.11998642742967407	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	576	0.11773795945399022	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	573	0.11712474091516735	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	572	0.11692033473555974	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	569	0.11630711619673686	No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC	568	0.11610271001712923	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	565	0.11548949147830638	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	560	0.11446746058026826	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	539	0.1101749308085082	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	538	0.10997052462890057	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	531	0.10853968137164723	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	521	0.10649561957557102	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	519	0.10608680721635577	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	518	0.10588240103674815	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	515	0.10526918249792527	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	505	0.10322512070184905	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	503	0.10281630834263382	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	2.044061796076219E-4	0.0
14	0.0	0.0	0.0	4.088123592152438E-4	0.0
15	2.044061796076219E-4	0.0	0.0	4.088123592152438E-4	0.0
16	2.044061796076219E-4	0.0	0.0	0.0016352494368609752	0.0
17	2.044061796076219E-4	0.0	0.0	0.0026572803348990847	0.0
18	2.044061796076219E-4	0.0	0.0	0.003679311232937194	0.0
19	2.044061796076219E-4	0.0	0.0	0.0051101544901905474	0.0
20	2.044061796076219E-4	0.0	0.0	0.007971841004697253	0.0
21	2.044061796076219E-4	0.0	0.0	0.012468776956064936	0.0
22	2.044061796076219E-4	0.0	0.0	0.018600962344293592	0.0
23	2.044061796076219E-4	0.0	0.0	0.02187146121801554	0.0
24	2.044061796076219E-4	0.0	0.0	0.025550772450952736	0.0
25	2.044061796076219E-4	0.0	0.0	0.027390428067421334	0.0
26	2.044061796076219E-4	0.0	0.0	0.03127414547996615	0.0
27	2.044061796076219E-4	0.0	0.0	0.049875107824259746	0.0
28	2.044061796076219E-4	0.0	0.0	0.17578931446255483	0.0
29	4.088123592152438E-4	0.0	0.0	0.36220775026470603	0.0
30	4.088123592152438E-4	0.0	0.0	0.5970704506338635	0.0
31	4.088123592152438E-4	0.0	0.0	1.2153991439469198	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATTTT	20	0.0018409026	37.0	18
GGTATCA	575	0.0	30.886957	1
GTTTAGG	30	3.596482E-4	30.833334	1
CTTATTG	105	0.0	28.190475	28
ATACGGC	90	5.456968E-12	26.722221	29
CTTTATG	35	8.8620826E-4	26.428572	1
ACACGAA	50	9.077157E-6	25.900002	17
CACGAAA	50	9.077157E-6	25.900002	18
AAACACG	50	9.077157E-6	25.900002	15
CTTTTTA	45	1.3216947E-4	24.666666	2
TAAACCA	45	1.3216947E-4	24.666666	5
TACGAGG	45	1.3216947E-4	24.666666	7
GACAGGC	340	0.0	23.941175	7
TCACGTA	85	1.9699655E-9	23.941175	25
GTGATTC	125	0.0	23.68	16
GGACGTA	55	1.8997638E-5	23.545454	27
CATCAGA	350	0.0	23.257141	16
TAAAGTG	40	0.0019296102	23.125002	5
CCAGGAC	400	0.0	23.125002	3
GAACTAA	40	0.0019296102	23.125002	11
>>END_MODULE