FastQCFastQC Report
Fri 10 Feb 2017
ERR1631962.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631962.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631810
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA17300.2738164954654089No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA17190.27207546572545543No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC15260.24152830756081733No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA15020.23772969721910067No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG14450.22870799765752362No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13930.22047767525047085No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT13560.21462148430699104No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13510.21383010715246673No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA13390.2119308019816084No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA13330.2109811493961792No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC13170.20844874250170145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12820.20290910242003135No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT12800.20259255155822162No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA11470.18154191924787513No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10970.1736281477026321No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9210.14577167186337664No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC8930.14133995979804054No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG8860.14023203178170654No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8570.13564204428546556No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA8480.13421756540732183No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA8450.13374273911460724No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8350.13215998480555863No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT8220.13010240420379546No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8030.1270951710166031No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7940.12567069213845933No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7840.12408793782941074No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7840.12408793782941074No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA7660.12123898007312325No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7370.11664899257688228No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA7340.11617416628416771No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7310.11569933999145313No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT7250.11474968740602395No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6920.10952659818616356No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG6790.10746901758440038No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6760.10699419129168578No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA6680.1057279878444469No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6650.10525316155173232No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA6350.1005048986245865No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACTT358.864957E-426.4285714
GGTATCA12000.026.3625011
CGTCAGA701.9200706E-723.7857131
GTATAAG652.6796715E-622.7692311
TATAAGA652.6796715E-622.7692312
TATACAC1750.022.237
GTGTATA855.171387E-821.7647069
GCAGTCG2300.021.7173929
CAGTCGG2400.021.58333410
CTGTGCA2100.021.1428579
GCTTATT806.949904E-720.812527
ATACGGC806.949904E-720.812529
GTATTGC555.1395723E-420.1818181
GTTGCGG555.1395723E-420.18181818
ACGAAAG1750.020.08571419
CTTAAAC656.895844E-519.9230773
GGACGTA1950.019.92307727
TAAGTCT759.254942E-619.73333435
TAACCCT851.2434921E-619.58823635
TAGCGAA1800.019.52777710
Produced by FastQC (version 0.11.5)