##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631962.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	631810
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.608755796837656	34.0	31.0	34.0	31.0	34.0
2	32.78068406641237	34.0	31.0	34.0	31.0	34.0
3	32.864220256089645	34.0	31.0	34.0	31.0	34.0
4	36.29001915132714	37.0	37.0	37.0	35.0	37.0
5	36.206576344154094	37.0	35.0	37.0	35.0	37.0
6	36.26693151422105	37.0	37.0	37.0	35.0	37.0
7	36.259533720580556	37.0	37.0	37.0	35.0	37.0
8	36.23832639559361	37.0	37.0	37.0	35.0	37.0
9	38.07209287602286	39.0	38.0	39.0	37.0	39.0
10	38.01060287111632	39.0	38.0	39.0	35.0	39.0
11	38.0832607904275	39.0	38.0	39.0	37.0	39.0
12	38.01552523701746	39.0	38.0	39.0	35.0	39.0
13	38.071216030135645	39.0	38.0	39.0	37.0	39.0
14	39.443130054921575	40.0	39.0	41.0	37.0	41.0
15	39.45684462100948	40.0	39.0	41.0	37.0	41.0
16	39.39725550402811	40.0	39.0	41.0	37.0	41.0
17	39.37317547997024	40.0	39.0	41.0	37.0	41.0
18	39.40715246672259	40.0	39.0	41.0	37.0	41.0
19	39.42350073598075	40.0	39.0	41.0	37.0	41.0
20	39.4337063357655	40.0	39.0	41.0	37.0	41.0
21	39.38785394343236	40.0	39.0	41.0	37.0	41.0
22	39.36524113261898	40.0	39.0	41.0	36.0	41.0
23	39.30706066697267	40.0	39.0	41.0	36.0	41.0
24	39.31779174118802	40.0	39.0	41.0	36.0	41.0
25	39.27762143682436	40.0	39.0	41.0	36.0	41.0
26	39.214630980832844	40.0	39.0	41.0	36.0	41.0
27	39.10434624333265	40.0	39.0	41.0	36.0	41.0
28	39.09758155141577	40.0	39.0	41.0	35.0	41.0
29	39.09175068454124	40.0	39.0	41.0	35.0	41.0
30	39.03661543818553	40.0	39.0	41.0	35.0	41.0
31	38.95419667305044	40.0	38.0	41.0	35.0	41.0
32	38.90570424652981	40.0	38.0	41.0	35.0	41.0
33	38.84478719868315	40.0	38.0	41.0	35.0	41.0
34	38.81891866225606	40.0	38.0	41.0	35.0	41.0
35	38.74765831499976	40.0	38.0	41.0	35.0	41.0
36	38.70142131336952	40.0	38.0	41.0	35.0	41.0
37	38.651748152134346	40.0	38.0	41.0	35.0	41.0
38	38.60449343948339	40.0	38.0	41.0	35.0	41.0
39	38.52332979851538	40.0	38.0	41.0	35.0	41.0
40	38.22400721735965	40.0	38.0	41.0	34.0	41.0
41	38.28446051819376	40.0	37.0	41.0	34.0	41.0
42	38.25870752283123	40.0	37.0	41.0	34.0	41.0
43	37.796548012851964	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	2.0
18	6.0
19	7.0
20	19.0
21	38.0
22	69.0
23	141.0
24	245.0
25	452.0
26	757.0
27	1203.0
28	1968.0
29	2892.0
30	4039.0
31	5837.0
32	7819.0
33	10473.0
34	15827.0
35	23580.0
36	36244.0
37	66845.0
38	149727.0
39	303618.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.58099745176556	16.572070717462527	13.303524793846252	27.54340703692566
2	20.131052056789226	19.79424193982368	33.3051075481553	26.769598455231797
3	21.279182032573082	20.188347762776786	30.141339959798042	28.391130244852093
4	14.982035738592298	16.721799275098526	35.3759832861145	32.920181700194675
5	16.60499200709074	32.8524398157674	33.90892831705734	16.63363986008452
6	31.887751064402277	34.57067789367056	16.740950602238016	16.800620439689148
7	28.819740111742455	28.448742501701464	21.050948861208273	21.68056852534781
8	27.018249157183334	30.578813250819074	21.175353349899495	21.2275842420981
9	28.282553299251354	13.114069103053133	18.58485937227964	40.01851822541587
10	19.299472942815086	23.39231731058388	30.003640334910813	27.30456941169022
11	38.277330210031494	20.068216710719994	19.366265174656938	22.28818790459157
12	21.952960541935077	24.513540463113912	27.476298254221998	26.057200740729016
13	34.67767208496225	18.154191924787515	21.569617448283505	25.59851854196673
14	23.431569617448282	21.446637438470425	24.29891897880692	30.822873965274372
15	29.66002437441636	24.61277915829126	20.685332615818048	25.041863851474332
16	24.141276649625677	24.882322217122237	23.69557303619759	27.280828097054492
17	25.86204713442332	24.46827368987512	22.55978854402431	27.109890631677246
18	25.999905034741456	21.58876877542299	24.387711495544547	28.023614694291005
19	27.080926227821656	23.109637390987796	24.471280923062313	25.338155458128238
20	29.45489941596366	21.00647346512401	23.84435194124816	25.69427517766417
21	26.847153416375175	22.693689558569822	22.830756081733433	27.62840094332157
22	27.72906411737706	23.513398015226098	22.367958721767618	26.38957914562922
23	27.191877304885963	22.514838321647332	23.453886453205868	26.839397920260836
24	27.103559614441053	22.130862126272138	24.137952865576676	26.627625393710137
25	26.806476630632623	22.617875627166395	24.33801301024042	26.237634731960558
26	26.99324163910036	23.289280005064814	23.394533166616547	26.322945189218277
27	26.258210537978186	22.195121951219512	25.492790554122287	26.053876956680018
28	25.507351893765527	23.460850572165683	23.07165128757063	27.96014624649816
29	26.63094917775914	23.832481283930296	23.29956790807363	26.23700163023694
30	25.98154508475649	22.724078441303554	24.700463747012552	26.5939127269274
31	27.07063832481284	22.8166695683829	23.90687073645558	26.20582137034868
32	24.745572244820437	22.94946265491208	23.692407527579494	28.61255757268799
33	24.950697203273137	22.40578654975388	25.970307529162245	26.673208717810738
34	25.097260252291036	22.344692233424606	25.16547696301103	27.392570551273327
35	24.757917728431018	24.427122077839858	25.66990076130482	25.145059432424304
36	25.310457257719882	23.132745603899906	25.62764122125322	25.929155917126984
37	25.92725661195613	23.002168373403396	25.32438549563951	25.746189519000968
38	25.34749370855162	22.210158117155473	25.90984631455659	26.532501859736314
39	24.691125496589166	21.028948576312498	27.279245342745444	27.000680584352892
40	24.327725107231604	22.379038001930958	27.73222962599516	25.561007264842278
41	22.521169338883524	21.292318893338187	28.958547664646016	27.22796410313227
42	21.21270635159304	21.556638862949303	29.964229752615502	27.26642503284215
43	20.53876956680015	22.55868061600798	29.418654342286448	27.483895474905427
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	8.5
2	12.0
3	27.0
4	42.0
5	42.0
6	70.5
7	99.0
8	111.5
9	124.0
10	179.0
11	234.0
12	234.0
13	550.0
14	866.0
15	1671.5
16	2477.0
17	2386.5
18	2296.0
19	2296.0
20	2598.5
21	2901.0
22	2398.5
23	1896.0
24	2065.5
25	2235.0
26	2235.0
27	2502.5
28	2770.0
29	3416.5
30	4063.0
31	4733.5
32	5404.0
33	5404.0
34	6635.0
35	7866.0
36	8615.5
37	9365.0
38	12114.5
39	14864.0
40	14864.0
41	17446.0
42	20028.0
43	23658.0
44	27288.0
45	40148.0
46	53008.0
47	53008.0
48	59776.0
49	66544.0
50	73667.0
51	80790.0
52	79811.0
53	78832.0
54	78832.0
55	70640.5
56	62449.0
57	58503.0
58	54557.0
59	48498.5
60	42440.0
61	42440.0
62	37263.0
63	32086.0
64	25732.0
65	19378.0
66	16438.5
67	13499.0
68	13499.0
69	11395.5
70	9292.0
71	7668.0
72	6044.0
73	4713.5
74	3383.0
75	3383.0
76	2640.5
77	1898.0
78	1522.0
79	1146.0
80	914.0
81	682.0
82	682.0
83	565.5
84	449.0
85	358.5
86	268.0
87	205.0
88	142.0
89	142.0
90	108.5
91	75.0
92	43.0
93	11.0
94	6.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	631810.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.77383022188473
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.65335135398114	34.516413758207825
2	6.857296368784632	5.591964758439426
3	2.2684158977046924	2.7747601405690614
4	1.2136054343741565	1.979333677501141
5	0.8231770311053513	1.678204025442236
6	0.5728098196915828	1.4013390202519802
7	0.4381492428880211	1.2505515988954452
8	0.3186256502429696	1.0393270533875576
9	0.2684412633379322	0.9850840646300189
>10	2.086449606579479	17.171589462854605
>50	0.28268187289482044	8.045737748730984
>100	0.19705624331763497	16.232721084990846
>500	0.014075445951259641	4.014339935979282
>1k	0.005864769146358184	3.318633670119563
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1730	0.2738164954654089	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1719	0.27207546572545543	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1526	0.24152830756081733	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1502	0.23772969721910067	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1445	0.22870799765752362	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1393	0.22047767525047085	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1356	0.21462148430699104	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1351	0.21383010715246673	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1339	0.2119308019816084	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1333	0.2109811493961792	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1317	0.20844874250170145	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1282	0.20290910242003135	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1280	0.20259255155822162	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1147	0.18154191924787513	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1097	0.1736281477026321	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	921	0.14577167186337664	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	893	0.14133995979804054	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	886	0.14023203178170654	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	857	0.13564204428546556	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	848	0.13421756540732183	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	845	0.13374273911460724	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	835	0.13215998480555863	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	822	0.13010240420379546	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	803	0.1270951710166031	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	794	0.12567069213845933	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	784	0.12408793782941074	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	784	0.12408793782941074	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	766	0.12123898007312325	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	737	0.11664899257688228	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	734	0.11617416628416771	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	731	0.11569933999145313	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	725	0.11474968740602395	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	692	0.10952659818616356	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	679	0.10746901758440038	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	676	0.10699419129168578	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	668	0.1057279878444469	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	665	0.10525316155173232	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	635	0.1005048986245865	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.5827543090486063E-4	0.0
10	0.0	0.0	0.0	1.5827543090486063E-4	0.0
11	0.0	0.0	0.0	1.5827543090486063E-4	0.0
12	0.0	0.0	0.0	3.1655086180972127E-4	0.0
13	1.5827543090486063E-4	0.0	0.0	3.1655086180972127E-4	0.0
14	3.1655086180972127E-4	0.0	0.0	4.748262927145819E-4	0.0
15	4.748262927145819E-4	0.0	0.0	4.748262927145819E-4	0.0
16	6.331017236194425E-4	0.0	0.0	7.913771545243032E-4	0.0
17	6.331017236194425E-4	0.0	0.0	7.913771545243032E-4	0.0
18	6.331017236194425E-4	0.0	0.0	9.496525854291639E-4	0.0
19	6.331017236194425E-4	0.0	0.0	9.496525854291639E-4	0.0
20	6.331017236194425E-4	0.0	0.0	0.0015827543090486064	0.0
21	6.331017236194425E-4	0.0	0.0	0.003007233187192352	0.0
22	6.331017236194425E-4	0.0	0.0	0.003956885772621516	0.0
23	6.331017236194425E-4	0.0	0.0	0.00506481378895554	0.0
24	6.331017236194425E-4	0.0	0.0	0.006489292667099286	0.0
25	6.331017236194425E-4	0.0	0.0	0.00981307671610136	0.0
26	6.331017236194425E-4	0.0	0.0	0.012503759041483991	0.0
27	6.331017236194425E-4	0.0	0.0	0.0333961159209256	0.0
28	6.331017236194425E-4	0.0	0.0	0.1234548361057913	0.0
29	6.331017236194425E-4	0.0	0.0	0.27793165666893527	0.0
30	7.913771545243032E-4	0.0	0.0	0.47593422073091596	0.0
31	7.913771545243032E-4	0.0	0.0	1.0207182539054462	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACTT	35	8.864957E-4	26.42857	14
GGTATCA	1200	0.0	26.362501	1
CGTCAGA	70	1.9200706E-7	23.785713	1
GTATAAG	65	2.6796715E-6	22.76923	11
TATAAGA	65	2.6796715E-6	22.76923	12
TATACAC	175	0.0	22.2	37
GTGTATA	85	5.171387E-8	21.764706	9
GCAGTCG	230	0.0	21.717392	9
CAGTCGG	240	0.0	21.583334	10
CTGTGCA	210	0.0	21.142857	9
GCTTATT	80	6.949904E-7	20.8125	27
ATACGGC	80	6.949904E-7	20.8125	29
GTATTGC	55	5.1395723E-4	20.181818	1
GTTGCGG	55	5.1395723E-4	20.181818	18
ACGAAAG	175	0.0	20.085714	19
CTTAAAC	65	6.895844E-5	19.923077	3
GGACGTA	195	0.0	19.923077	27
TAAGTCT	75	9.254942E-6	19.733334	35
TAACCCT	85	1.2434921E-6	19.588236	35
TAGCGAA	180	0.0	19.527777	10
>>END_MODULE