FastQCFastQC Report
Fri 10 Feb 2017
ERR1631961.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631961.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271531
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5840.2150767315702443No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5380.19813575613834145No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5320.19592606369070198No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4920.18119478070643869No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4760.17530226751273334No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4300.15836129208083055No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4270.1572564458570108No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4250.15651988170779763No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4210.1550467534093713No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4170.15357362511094497No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4100.1509956505886989No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3970.14620798361881332No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3440.12668903366466444No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3420.12595246951545128No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3400.1252159053662381No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3240.1193233921725328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3200.11785026387410645No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG3150.11600885350107354No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2910.10717008371051556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.10643351956130238No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC2860.10532867333748264No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2800.10311898088984316No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2730.10054100636759708No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2730.10054100636759708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATTA352.3816436E-531.71428515
CAGTATT352.3816436E-531.71428514
GGTATCA4000.030.9875011
TCCAATC250.00548904429.62
TCTAGCA405.9236856E-527.753
TAGCATC405.9236856E-527.755
CTAGCAT405.9236856E-527.754
ATATGCC556.232185E-726.9090935
CCTAGGA358.8518864E-426.428572
CCTCTAG509.057738E-625.9000021
GATATGC659.336691E-825.61538334
CTCTATG1400.025.1071411
CTAAGTC451.3195784E-424.66666834
TCTATGG1500.023.4333342
GGCACTA400.001927410623.12526
CACTACG400.001927410623.12528
GCAGTCG1054.0017767E-1122.904769
TATGCCC652.6703347E-622.76922836
AGTCTGT652.6703347E-622.76922837
TATACAC903.796231E-922.61111337
Produced by FastQC (version 0.11.5)