FastQCFastQC Report
Fri 10 Feb 2017
ERR1631960.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631960.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334163
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5980.17895458204528927No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5520.1651888449648824No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4850.14513874965211587No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4560.13646035018838112No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4120.12329312341581802No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4040.12089908218444291No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4030.12059982703052102No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4000.11970206156875537No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3890.1164102548756146No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3720.11132291725894249No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3550.10623557964227039No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3490.10444004871873905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACACT250.005490835829.5999984
TACGGCT754.6020432E-1027.13333330
GGTATCA3050.026.6885261
GCGGTAA2550.025.39215723
ATACGGC851.9645086E-923.94117729
ATATTGC400.001928337423.1256
AGCCGCG2900.022.96551719
TTCTGCG2050.022.56097618
CGCGGTA2850.022.07017522
GGTAACG2150.021.51162725
GTAACGT2150.021.51162726
CGGTAAT2950.021.32203324
CGTGCCA3050.021.2295110
GCCTAAG705.0840736E-621.1428571
TGCGGGT2300.020.91304421
TATACAC1251.8189894E-1120.72000137
GATACTT450.003820215620.55555714
GACGTAT450.003820215620.5555571
TAAGTCT450.003820215620.55555735
ATAACGC450.003820215620.55555735
Produced by FastQC (version 0.11.5)