##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631960.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	334163
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.577670178924656	34.0	31.0	34.0	31.0	34.0
2	32.74658475055587	34.0	31.0	34.0	31.0	34.0
3	32.839835649069464	34.0	31.0	34.0	31.0	34.0
4	36.28097964167188	37.0	37.0	37.0	35.0	37.0
5	36.190380143822026	37.0	35.0	37.0	35.0	37.0
6	36.232195066479534	37.0	37.0	37.0	35.0	37.0
7	36.222526132456316	37.0	36.0	37.0	35.0	37.0
8	36.20393939484623	37.0	36.0	37.0	35.0	37.0
9	38.040866283819575	39.0	38.0	39.0	35.0	39.0
10	37.97182213470671	39.0	38.0	39.0	35.0	39.0
11	38.04278151680467	39.0	38.0	39.0	35.0	39.0
12	37.98082672228823	39.0	38.0	39.0	35.0	39.0
13	38.02944371459437	39.0	38.0	39.0	35.0	39.0
14	39.40948279731748	40.0	39.0	41.0	37.0	41.0
15	39.41224192983664	40.0	39.0	41.0	37.0	41.0
16	39.361844369364654	40.0	39.0	41.0	36.0	41.0
17	39.3268015908404	40.0	39.0	41.0	36.0	41.0
18	39.35185523232674	40.0	39.0	41.0	36.0	41.0
19	39.3782615071088	40.0	39.0	41.0	37.0	41.0
20	39.371674901170984	40.0	39.0	41.0	36.0	41.0
21	39.32339307463723	40.0	39.0	41.0	36.0	41.0
22	39.319359115162364	40.0	39.0	41.0	36.0	41.0
23	39.23746794229164	40.0	39.0	41.0	36.0	41.0
24	39.26138740674461	40.0	39.0	41.0	36.0	41.0
25	39.203613206728456	40.0	39.0	41.0	36.0	41.0
26	39.14272376055997	40.0	39.0	41.0	36.0	41.0
27	39.029814790985235	40.0	39.0	41.0	35.0	41.0
28	39.03475549357649	40.0	39.0	41.0	35.0	41.0
29	39.01670143014038	40.0	39.0	41.0	35.0	41.0
30	38.978112478042156	40.0	38.0	41.0	35.0	41.0
31	38.90559996169534	40.0	38.0	41.0	35.0	41.0
32	38.860642859921654	40.0	38.0	41.0	35.0	41.0
33	38.8122084132594	40.0	38.0	41.0	35.0	41.0
34	38.78070881575758	40.0	38.0	41.0	35.0	41.0
35	38.72372464934777	40.0	38.0	41.0	35.0	41.0
36	38.689651457522224	40.0	38.0	41.0	35.0	41.0
37	38.64715722566532	40.0	38.0	41.0	35.0	41.0
38	38.619975880034595	40.0	38.0	41.0	35.0	41.0
39	38.54774466353247	40.0	38.0	41.0	35.0	41.0
40	38.25532748987769	40.0	38.0	41.0	34.0	41.0
41	38.341689534748014	40.0	38.0	41.0	34.0	41.0
42	38.33078168438756	40.0	37.0	41.0	34.0	41.0
43	37.8597151689445	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	5.0
20	10.0
21	22.0
22	42.0
23	88.0
24	144.0
25	275.0
26	476.0
27	737.0
28	1052.0
29	1485.0
30	2158.0
31	3069.0
32	4187.0
33	5936.0
34	8491.0
35	12993.0
36	19718.0
37	34965.0
38	80352.0
39	157955.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.823412526222235	16.386613718454765	12.826075897092137	27.96389785823086
2	20.717434306012336	19.182255366393047	32.92854086179499	27.17176946579962
3	20.97479373838516	19.828646498864327	29.061266507662424	30.135293255088087
4	15.730047910750141	15.632789985725529	33.461813546083796	35.17534855744053
5	16.916893851204353	32.957269356571494	33.38131390967881	16.744522882545347
6	34.816242372734266	33.438471644077886	15.35567971319386	16.389606269993983
7	30.68592273830436	27.68289726869821	19.896876673958516	21.73430331903891
8	27.36927786738807	31.19764905151079	20.010892887602758	21.422180193498384
9	28.021654102937788	13.281242986207332	17.732364145641498	40.96473876521338
10	19.0161687559664	23.7288389199283	30.05988095629977	27.19511136780553
11	38.843618234215036	19.725104215607352	19.181656856085205	22.249620694092403
12	22.990576455202998	23.531330518339853	26.80039381978256	26.67769920667459
13	34.11209499555606	17.875707364370083	21.44103326819546	26.571164371878396
14	24.087645849480644	19.550039950563047	23.262300134964075	33.10001406499224
15	29.084907664822257	24.256126501138667	20.243114887046143	26.415850946992936
16	25.884673048781583	24.124454233413033	22.331317351113082	27.659555366692302
17	26.750118953923685	23.652528855678217	22.229271343625715	27.368080846772386
18	26.43410551138217	22.099993117131458	23.6181145129772	27.84778685850917
19	28.089285767724135	22.427378255522008	23.497215430792757	25.986120545961107
20	29.288999679796984	21.248612204223686	23.08394406322663	26.378444052752698
21	27.311222367527225	22.571918494866278	22.117649171212854	27.999209966393646
22	28.020457082322096	22.81461442469693	21.670861226407474	27.494067266573495
23	27.870230995053312	22.61531049218495	22.451318667835757	27.06313984492598
24	27.80020528903559	22.082636318203992	22.610821664876124	27.506336727884296
25	27.886690028519016	22.186178601460963	23.073170877685442	26.85396049233458
26	27.588931150366736	22.828380161777336	22.639849414806545	26.94283927304938
27	27.224737628043798	22.080840787280458	23.758165925012644	26.9362556596631
28	26.319790042584007	23.111774792541365	22.63595909780556	27.932476067069068
29	27.016755296068084	23.448436840703486	22.76852913099296	26.766278732235467
30	26.744133850845248	22.604836561797686	23.668688633989998	26.982340953367068
31	27.479403764031325	22.67157046112227	22.93521425172745	26.91381152311896
32	25.4597307302125	22.44383728898771	23.58489719089187	28.511534789907923
33	25.898438785861988	21.924928852087156	24.681966585169512	27.494665776881344
34	26.308119091581055	21.756448200429134	24.424008642488847	27.511424065500968
35	25.893949958553158	22.940600844498043	24.6352827811577	26.530166415791097
36	25.82272723191975	21.766323620508555	25.448957544671313	26.961991602900383
37	26.122580896149483	21.167214802356934	25.31997857333098	27.390225728162605
38	25.19937874630046	20.672845288077973	26.604680949117647	27.523095016503923
39	24.65323809039301	19.960318766589957	27.605988694140283	27.780454448876746
40	24.233981619748445	20.78596373626045	28.504352666213794	26.475701977777312
41	22.71975054090369	20.054883395229275	29.494288715387402	27.731077348479634
42	21.620287105394674	20.613293512447516	29.98207461628008	27.784344765877727
43	20.91314717667725	21.13280045965592	29.93030347465159	28.023748889015245
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	4.0
2	3.0
3	4.0
4	5.0
5	5.0
6	12.5
7	20.0
8	18.5
9	17.0
10	32.0
11	47.0
12	47.0
13	99.0
14	151.0
15	286.5
16	422.0
17	420.5
18	419.0
19	419.0
20	511.0
21	603.0
22	568.5
23	534.0
24	650.5
25	767.0
26	767.0
27	920.0
28	1073.0
29	1534.0
30	1995.0
31	2506.0
32	3017.0
33	3017.0
34	3888.0
35	4759.0
36	5365.5
37	5972.0
38	7725.0
39	9478.0
40	9478.0
41	11100.5
42	12723.0
43	14394.0
44	16065.0
45	20137.0
46	24209.0
47	24209.0
48	26850.5
49	29492.0
50	32952.0
51	36412.0
52	37584.5
53	38757.0
54	38757.0
55	35112.0
56	31467.0
57	29629.5
58	27792.0
59	25753.0
60	23714.0
61	23714.0
62	21676.5
63	19639.0
64	16648.5
65	13658.0
66	11940.5
67	10223.0
68	10223.0
69	8831.0
70	7439.0
71	6332.0
72	5225.0
73	4202.0
74	3179.0
75	3179.0
76	2623.0
77	2067.0
78	1655.0
79	1243.0
80	985.5
81	728.0
82	728.0
83	574.5
84	421.0
85	326.5
86	232.0
87	167.5
88	103.0
89	103.0
90	83.0
91	63.0
92	43.5
93	24.0
94	12.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	334163.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.642592936431036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.12012274482454	50.218297719518276
2	6.142825294291622	7.0817675583692825
3	2.0754006860082947	3.588944307706975
4	1.0676175988858498	2.461609866573878
5	0.6526290664269134	1.8809615807264788
6	0.4604422542139867	1.592465125823372
7	0.31390920930168653	1.2666178539541835
8	0.26503367859344107	1.2221782759685293
9	0.20568953955745253	1.067083055999295
>10	1.4823889549010647	16.68820047650011
>50	0.14662783758937856	5.775038010748129
>100	0.06626952090338459	6.810935453926073
>500	0.0010436145024155052	0.34590071418543467
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	598	0.17895458204528927	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	552	0.1651888449648824	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	485	0.14513874965211587	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	456	0.13646035018838112	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	412	0.12329312341581802	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	404	0.12089908218444291	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	403	0.12059982703052102	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	400	0.11970206156875537	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	389	0.1164102548756146	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	372	0.11132291725894249	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	355	0.10623557964227039	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	349	0.10444004871873905	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	2.992551539218884E-4	0.0	0.0	0.0	0.0
11	2.992551539218884E-4	0.0	0.0	0.0	0.0
12	2.992551539218884E-4	0.0	0.0	0.0	0.0
13	2.992551539218884E-4	0.0	0.0	0.0	0.0
14	2.992551539218884E-4	0.0	0.0	0.0	0.0
15	2.992551539218884E-4	0.0	0.0	0.0	0.0
16	2.992551539218884E-4	0.0	0.0	0.0	0.0
17	2.992551539218884E-4	0.0	0.0	5.985103078437769E-4	0.0
18	2.992551539218884E-4	0.0	0.0	5.985103078437769E-4	0.0
19	2.992551539218884E-4	0.0	0.0	5.985103078437769E-4	0.0
20	2.992551539218884E-4	0.0	0.0	0.0011970206156875537	0.0
21	2.992551539218884E-4	0.0	0.0	0.0026932963852969957	0.0
22	2.992551539218884E-4	0.0	0.0	0.004189572154906438	0.0
23	2.992551539218884E-4	0.0	0.0	0.004788082462750215	0.0
24	2.992551539218884E-4	0.0	0.0	0.007481378848047211	0.0
25	2.992551539218884E-4	0.0	0.0	0.008977654617656653	0.0
26	2.992551539218884E-4	0.0	0.0	0.01137169584903176	0.0
27	2.992551539218884E-4	0.0	0.0	0.04189572154906438	0.0
28	2.992551539218884E-4	0.0	0.0	0.16159778311781975	0.0
29	2.992551539218884E-4	0.0	0.0	0.34384417185624977	0.0
30	5.985103078437769E-4	0.0	0.0	0.5850438259172919	0.0
31	5.985103078437769E-4	0.0	0.0	1.3029569401759022	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACACT	25	0.0054908358	29.599998	4
TACGGCT	75	4.6020432E-10	27.133333	30
GGTATCA	305	0.0	26.688526	1
GCGGTAA	255	0.0	25.392157	23
ATACGGC	85	1.9645086E-9	23.941177	29
ATATTGC	40	0.0019283374	23.125	6
AGCCGCG	290	0.0	22.965517	19
TTCTGCG	205	0.0	22.560976	18
CGCGGTA	285	0.0	22.070175	22
GGTAACG	215	0.0	21.511627	25
GTAACGT	215	0.0	21.511627	26
CGGTAAT	295	0.0	21.322033	24
CGTGCCA	305	0.0	21.22951	10
GCCTAAG	70	5.0840736E-6	21.142857	1
TGCGGGT	230	0.0	20.913044	21
TATACAC	125	1.8189894E-11	20.720001	37
GATACTT	45	0.0038202156	20.555557	14
GACGTAT	45	0.0038202156	20.555557	1
TAAGTCT	45	0.0038202156	20.555557	35
ATAACGC	45	0.0038202156	20.555557	35
>>END_MODULE