FastQCFastQC Report
Fri 10 Feb 2017
ERR1631958.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631958.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193057
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4200.21755232910487576No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4190.21703434736891178No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4040.20926462132945192No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3610.18699140668300035No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3470.1797396623795045No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3460.17922168064354052No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3060.1585024112049809No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2990.15487653905323298No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2920.15125066690148506No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2880.1491787399576291No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2820.14607084954184515No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2690.1393370869743133No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2630.13622919655852933No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2440.12638754357521353No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2180.11292001844014982No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2060.10670423760858191No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2050.10618625587261793No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2040.10566827413665393No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2020.10463231066472596No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1990.103078365456834No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1990.103078365456834No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGTTG250.00548516129.623
TGTGCCA250.00548516129.612
GCAGTCG405.9149505E-527.7500029
CAGTCGG405.9149505E-527.75000210
AGAAGAG358.8425784E-426.428575
GGTATCA2950.026.3389841
GGGCAGT451.317649E-424.6666667
AAATCTC400.001925403523.1250029
GTGATTC400.001925403523.12500216
ATCTAAG1053.8198777E-1122.9047632
CATCTAA1107.2759576E-1121.86363631
CGCCTTA603.705655E-521.58333225
ACATCTA1151.3278623E-1020.91304430
CTCTATG806.89859E-720.81251
CACTCGT450.003814460720.55555517
TATTAGA450.003814460720.5555552
AACCTAT450.003814460720.55555536
AGTCGGT450.003814460720.55555511
CTTATAC2000.020.3537
TCTAAGT1101.7280399E-920.18181833
Produced by FastQC (version 0.11.5)