FastQCFastQC Report
Fri 10 Feb 2017
ERR1631957.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631957.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568412
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA19060.3353201550987664No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA17260.30365298410308017No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12770.2246609853416184No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12330.21692012132045066No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11080.1949290303512241No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10740.1889474536075945No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9210.1620303582612612No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9090.15991921352821545No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8860.15587285278987778No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8780.15446542296784727No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8640.15200242077929388No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT8620.1516505633237863No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA8600.15129870586827865No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8540.15024313350175578No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC8510.1497153473184943No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8420.14813198876871003No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8350.14690048767443334No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT8180.14390969930261854No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA8040.14144669711406516No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC8000.1407429822030499No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7880.13863183747000415No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC7740.1361688352814508No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC7690.13528919164268172No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT7690.13528919164268172No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7640.13440954800391264No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC7520.1322984032708669No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA7300.12842797126028305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7050.12402975306643772No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7040.1238538243386839No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7040.1238538243386839No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6960.12244639451665341No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6950.1222704657888996No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC6820.11998339232810004No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6800.11963153487259241No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6720.11822410505056191No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6690.11769631886730048No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6540.1150573879509933No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA6510.11452960176773187No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC6490.11417774431222423No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG6430.11312217194570137No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG6380.1122425283069323No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6370.11206659957917849No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC6340.11153881339591705No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6110.10749245265757937No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC6080.10696466647431792No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA5850.10291830573598024No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC5810.102214590824965No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTTC250.005494034429.613
TGTTCTA502.716697E-729.615
AGTCGGG250.005494034429.621
GGTATCA10400.029.1730791
ATGTTCT405.93493E-527.7514
TACCGCT601.3354202E-624.66666835
ATACGGC1250.023.6829
TATTAGC551.900466E-523.5454542
GTATTGC400.001929993323.1251
TACGGCT1300.022.76923430
TATACAC2200.021.86363637
AGGACAC603.7220212E-521.5833345
AAGAGAG705.094438E-621.1428595
GCGGTAA7800.020.63461723
ATACCCA450.003823464720.5555554
GGGCTAA555.138699E-420.1818181
TTAAGCT555.138699E-420.18181836
CTTAGGA1101.7498678E-920.1818182
GCTCATA555.138699E-420.18181834
GTATTAG1355.638867E-1119.1851841
Produced by FastQC (version 0.11.5)