##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631957.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	568412
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54311309402335	34.0	31.0	34.0	31.0	34.0
2	32.71167216737155	34.0	31.0	34.0	31.0	34.0
3	32.801585821551974	34.0	31.0	34.0	31.0	34.0
4	36.23714136928847	37.0	37.0	37.0	35.0	37.0
5	36.161361829095796	37.0	35.0	37.0	35.0	37.0
6	36.21486703306756	37.0	36.0	37.0	35.0	37.0
7	36.21715586581564	37.0	36.0	37.0	35.0	37.0
8	36.19838778913886	37.0	36.0	37.0	35.0	37.0
9	38.04424079716825	39.0	38.0	39.0	35.0	39.0
10	37.963579234780404	39.0	38.0	39.0	35.0	39.0
11	38.03705938650134	39.0	38.0	39.0	35.0	39.0
12	37.95575040639536	39.0	38.0	39.0	35.0	39.0
13	38.018342329155615	39.0	38.0	39.0	35.0	39.0
14	39.34457224689134	40.0	39.0	41.0	36.0	41.0
15	39.36310106049837	40.0	39.0	41.0	36.0	41.0
16	39.30432855041766	40.0	39.0	41.0	36.0	41.0
17	39.29132917672393	40.0	39.0	41.0	36.0	41.0
18	39.33077415677361	40.0	39.0	41.0	36.0	41.0
19	39.34351843381209	40.0	39.0	41.0	36.0	41.0
20	39.34566476429069	40.0	39.0	41.0	36.0	41.0
21	39.300289226828426	40.0	39.0	41.0	36.0	41.0
22	39.26828779125001	40.0	39.0	41.0	36.0	41.0
23	39.19244667600262	40.0	39.0	41.0	36.0	41.0
24	39.20684644236927	40.0	39.0	41.0	36.0	41.0
25	39.1494408985032	40.0	39.0	41.0	36.0	41.0
26	39.07799624216238	40.0	39.0	41.0	35.0	41.0
27	38.91758794677101	40.0	38.0	41.0	35.0	41.0
28	38.92454416866639	40.0	38.0	41.0	35.0	41.0
29	38.89726641942816	40.0	38.0	41.0	35.0	41.0
30	38.859841101173096	40.0	38.0	41.0	35.0	41.0
31	38.764829032462366	40.0	38.0	41.0	35.0	41.0
32	38.70894879066593	40.0	38.0	41.0	35.0	41.0
33	38.64460285848997	40.0	38.0	41.0	35.0	41.0
34	38.61307467118921	40.0	38.0	41.0	35.0	41.0
35	38.53986017184718	40.0	38.0	41.0	35.0	41.0
36	38.473007255300736	40.0	38.0	41.0	34.0	41.0
37	38.421136429209795	40.0	38.0	41.0	34.0	41.0
38	38.38333462347734	40.0	38.0	41.0	34.0	41.0
39	38.304193085297285	40.0	37.0	41.0	34.0	41.0
40	38.013124283090434	40.0	37.0	41.0	34.0	41.0
41	38.068079139778895	40.0	37.0	41.0	34.0	41.0
42	38.04577489567426	40.0	37.0	41.0	34.0	41.0
43	37.579167927489216	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	0.0
14	0.0
15	2.0
16	0.0
17	3.0
18	2.0
19	13.0
20	26.0
21	32.0
22	102.0
23	186.0
24	311.0
25	498.0
26	856.0
27	1325.0
28	1995.0
29	2960.0
30	4167.0
31	5757.0
32	7606.0
33	10744.0
34	15161.0
35	23588.0
36	36231.0
37	63162.0
38	138260.0
39	255422.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.81383925744003	16.596060603928137	12.740406606475585	27.849693532156255
2	21.696234421511157	19.737268037972456	31.89922098759351	26.667276552922882
3	21.155605441123694	19.71562880445874	30.088738450279024	29.040027304138547
4	14.915765325151476	16.406937221592788	35.73780989845394	32.9394875548018
5	16.726599719921463	32.89093122594174	33.47853317663948	16.903935877497307
6	31.865970458048036	35.21143114501453	16.076015284687866	16.846583112249565
7	29.158075480461353	28.43606398175971	19.924983990485774	22.48087654729316
8	27.03584723756712	29.74919600571416	20.832424368239938	22.38253238847878
9	28.347923689155046	12.417401462319585	17.88509039218032	41.34958445634504
10	19.838251127703145	22.72770455233176	29.30638339795782	28.127660922007276
11	39.24670837350372	19.476013877258044	19.002941528328044	22.274336220910186
12	22.659268277235526	24.405536828919868	26.627868517906027	26.30732637593858
13	36.166020421806714	17.303470018226218	20.474761264716438	26.05574829525063
14	23.7910177828758	20.431482797689	23.674025178919518	32.10347424051568
15	30.160517371202577	24.077605680386764	19.987614617566134	25.77426233084453
16	24.567743115908883	24.27816443002611	22.91102228665123	28.243070167413777
17	26.75488905934428	23.463086634342694	21.69447513423362	28.087549172079406
18	26.876983596405424	20.595096514500046	23.51727268249087	29.01064720660366
19	28.62114100335672	21.955377437492523	23.351899678402287	26.071581880748475
20	30.715572507265858	20.04250438062532	22.519932724854506	26.721990387254312
21	27.93959311203845	21.667382110159533	21.949219932021137	28.44380484578088
22	28.469314511305182	22.858243668325088	20.95012068710724	27.722321133262497
23	28.479870234970413	21.606158912901204	22.378661956468196	27.535308895660187
24	27.72883049618938	21.199059836878885	22.822178279135557	28.249931387796174
25	27.992019872909086	21.862838926694018	23.106479103185716	27.03866209721118
26	28.07241930149258	22.60367479926532	22.352272647305124	26.97163325193698
27	26.68029527877666	22.05776795704524	24.567039400997867	26.69489736318023
28	26.42414305116711	23.004088583633	22.24196533500348	28.329803030196405
29	26.910234125950893	23.9803170939389	22.44586673047015	26.66358204964005
30	26.653554112158083	22.09365741750702	24.286784937686047	26.966003532648852
31	28.189763762904374	22.634110469166732	22.511136288466815	26.664989479462083
32	24.78395952231832	22.319198046487408	23.25654630795972	29.640296123234556
33	25.28289339422813	21.878848440919615	25.294680618987638	27.54357754586462
34	25.530425114177746	21.776809778822404	24.9329711547258	27.75979395227405
35	25.561036712806906	23.78503620613217	25.032722743362207	25.62120433769871
36	25.96971914737901	22.442700013370583	25.408506505844354	26.17907433340605
37	26.336530544745713	22.36177279860383	24.6423368964765	26.659359760173956
38	25.32370885906701	21.156485084762462	26.11345291795388	27.406353138216645
39	24.6008177167266	20.324166273759175	27.113959592689806	27.961056416824416
40	24.722912253787747	21.304793002258926	28.030724193014926	25.941570550938405
41	23.253379590860153	20.081560558186666	28.890311956820053	27.774747894133128
42	21.148920149469046	21.232838152607616	30.37040034341288	27.24784135451046
43	20.397000767049253	21.350886328930425	29.833114008852736	28.41899889516759
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11.0
1	7.5
2	4.0
3	18.0
4	32.0
5	32.0
6	41.5
7	51.0
8	55.0
9	59.0
10	90.0
11	121.0
12	121.0
13	267.5
14	414.0
15	798.5
16	1183.0
17	1159.0
18	1135.0
19	1135.0
20	1327.5
21	1520.0
22	1331.5
23	1143.0
24	1224.0
25	1305.0
26	1305.0
27	1501.5
28	1698.0
29	2117.0
30	2536.0
31	3231.5
32	3927.0
33	3927.0
34	5285.5
35	6644.0
36	7579.0
37	8514.0
38	10502.5
39	12491.0
40	12491.0
41	15493.5
42	18496.0
43	22097.0
44	25698.0
45	35297.5
46	44897.0
47	44897.0
48	50729.5
49	56562.0
50	60975.5
51	65389.0
52	65650.5
53	65912.0
54	65912.0
55	60402.0
56	54892.0
57	52055.0
58	49218.0
59	46579.5
60	43941.0
61	43941.0
62	38861.0
63	33781.0
64	26810.5
65	19840.0
66	17113.5
67	14387.0
68	14387.0
69	12275.5
70	10164.0
71	9027.0
72	7890.0
73	5971.0
74	4052.0
75	4052.0
76	3426.0
77	2800.0
78	2686.0
79	2572.0
80	2450.0
81	2328.0
82	2328.0
83	1875.5
84	1423.0
85	1019.5
86	616.0
87	554.0
88	492.0
89	492.0
90	339.0
91	186.0
92	127.0
93	68.0
94	41.5
95	15.0
96	15.0
97	9.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	568412.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.01693738979283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.12723772533525	30.400988168753667
2	7.168536781856284	5.307145544608021
3	2.7737007577397583	3.0802172186182064
4	1.6345240083506547	2.4202029151691757
5	1.045808578003676	1.9356315326835176
6	0.7434902331161262	1.6513038845509271
7	0.5501858821864258	1.425633744755
8	0.4495496159112159	1.3312759988632714
9	0.35448219432551686	1.180966067382963
>10	2.572943606319075	19.036564890777363
>50	0.3345160125370643	8.659427622717102
>100	0.21822503822154954	15.853119306837032
>500	0.02392818401552078	6.242929684794323
>1k	0.0028713820818624942	1.4745934194894565
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1906	0.3353201550987664	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1726	0.30365298410308017	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1277	0.2246609853416184	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1233	0.21692012132045066	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1108	0.1949290303512241	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1074	0.1889474536075945	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	921	0.1620303582612612	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	909	0.15991921352821545	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	886	0.15587285278987778	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	878	0.15446542296784727	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	864	0.15200242077929388	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	862	0.1516505633237863	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	860	0.15129870586827865	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	854	0.15024313350175578	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	851	0.1497153473184943	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	842	0.14813198876871003	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	835	0.14690048767443334	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	818	0.14390969930261854	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	804	0.14144669711406516	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	800	0.1407429822030499	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	788	0.13863183747000415	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	774	0.1361688352814508	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	769	0.13528919164268172	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	769	0.13528919164268172	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	764	0.13440954800391264	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	752	0.1322984032708669	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	730	0.12842797126028305	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	705	0.12402975306643772	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	704	0.1238538243386839	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	704	0.1238538243386839	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	696	0.12244639451665341	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	695	0.1222704657888996	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	682	0.11998339232810004	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	680	0.11963153487259241	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	672	0.11822410505056191	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	669	0.11769631886730048	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	654	0.1150573879509933	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	651	0.11452960176773187	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	649	0.11417774431222423	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	643	0.11312217194570137	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	638	0.1122425283069323	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	637	0.11206659957917849	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	634	0.11153881339591705	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	611	0.10749245265757937	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	608	0.10696466647431792	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	585	0.10291830573598024	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	581	0.102214590824965	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7592872775381237E-4	0.0	0.0	0.0	0.0
2	1.7592872775381237E-4	0.0	0.0	0.0	0.0
3	1.7592872775381237E-4	0.0	0.0	0.0	0.0
4	1.7592872775381237E-4	0.0	0.0	0.0	0.0
5	1.7592872775381237E-4	0.0	0.0	0.0	0.0
6	1.7592872775381237E-4	0.0	0.0	0.0	0.0
7	1.7592872775381237E-4	0.0	0.0	0.0	0.0
8	3.5185745550762475E-4	0.0	0.0	0.0	0.0
9	3.5185745550762475E-4	0.0	0.0	0.0	0.0
10	5.277861832614371E-4	0.0	0.0	0.0	0.0
11	5.277861832614371E-4	0.0	0.0	0.0	0.0
12	5.277861832614371E-4	0.0	0.0	0.0	0.0
13	5.277861832614371E-4	0.0	0.0	0.0	0.0
14	5.277861832614371E-4	0.0	0.0	1.7592872775381237E-4	0.0
15	5.277861832614371E-4	0.0	0.0	7.037149110152495E-4	0.0
16	5.277861832614371E-4	0.0	0.0	7.037149110152495E-4	0.0
17	8.796436387690619E-4	0.0	0.0	0.0010555723665228742	0.0
18	8.796436387690619E-4	0.0	0.0	0.0010555723665228742	0.0
19	8.796436387690619E-4	0.0	0.0	0.001407429822030499	0.0
20	8.796436387690619E-4	0.0	0.0	0.002814859644060998	0.0
21	8.796436387690619E-4	0.0	0.0	0.0052778618326143716	0.0
22	8.796436387690619E-4	0.0	0.0	0.009148293843198244	0.0
23	8.796436387690619E-4	0.0	0.0	0.01108350984849018	0.0
24	8.796436387690619E-4	0.0	0.0	0.013194654581535929	0.0
25	8.796436387690619E-4	0.0	0.0	0.015305799314581677	0.0
26	8.796436387690619E-4	0.0	0.0	0.017944730230888863	0.0
27	8.796436387690619E-4	0.0	0.0	0.042574752116422594	0.0
28	8.796436387690619E-4	0.0	0.0	0.1660767189995989	0.0
29	8.796436387690619E-4	0.0	0.0	0.37015404319402123	0.0
30	0.0010555723665228742	0.0	0.0	0.6080096831171755	0.0
31	0.0010555723665228742	0.0	0.0	1.1781946897672815	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATGTTC	25	0.0054940344	29.6	13
TGTTCTA	50	2.716697E-7	29.6	15
AGTCGGG	25	0.0054940344	29.6	21
GGTATCA	1040	0.0	29.173079	1
ATGTTCT	40	5.93493E-5	27.75	14
TACCGCT	60	1.3354202E-6	24.666668	35
ATACGGC	125	0.0	23.68	29
TATTAGC	55	1.900466E-5	23.545454	2
GTATTGC	40	0.0019299933	23.125	1
TACGGCT	130	0.0	22.769234	30
TATACAC	220	0.0	21.863636	37
AGGACAC	60	3.7220212E-5	21.583334	5
AAGAGAG	70	5.094438E-6	21.142859	5
GCGGTAA	780	0.0	20.634617	23
ATACCCA	45	0.0038234647	20.555555	4
GGGCTAA	55	5.138699E-4	20.181818	1
TTAAGCT	55	5.138699E-4	20.181818	36
CTTAGGA	110	1.7498678E-9	20.181818	2
GCTCATA	55	5.138699E-4	20.181818	34
GTATTAG	135	5.638867E-11	19.185184	1
>>END_MODULE