FastQCFastQC Report
Fri 10 Feb 2017
ERR1631956.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631956.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845399
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC19800.23420893566233222No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA16060.18996947003722503No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT15680.18547455106996813No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA15140.17908703464281364No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA13270.15696730183026003No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC12560.1485689006019643No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12430.14703116516579745No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11890.14064364873864293No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC11890.14064364873864293No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG11840.14005221203242493No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT11250.1330732588990524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11240.1329549715578088No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10700.1265674551306543No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10690.12644916778941068No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT10410.12313712223458982No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG10100.11947021465603816No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT9390.11107181342774239No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9300.11000722735654998No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT9150.10823291723789595No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC8720.10314656156442106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8690.10279169954069026No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG8630.10208197549322864No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACTAG250.00549553229.59999816
GGTATCA10750.028.2232551
AAGAGCG358.867447E-426.428575
ATACACA1300.025.61538537
TTACACC451.3228077E-424.6666664
CTATATC1059.822543E-1021.1428594
TCGCTTA705.0992894E-621.14285723
GTACTAT806.9556336E-720.81251
CTTATAC9300.019.89247337
CCGCTTA851.2445107E-619.58823625
CTTAGTC851.2445107E-619.58823618
TATACAC2750.019.50909237
CCAGGAC5450.019.0091743
GACAGGC4950.018.6868697
CATCAGA4950.018.68686916
GCGGTAA5150.018.67961123
CAACCAA701.2187132E-418.510
TGCGGGT4400.018.521
TACCTTA801.6158467E-518.531
TCTATCG701.2187132E-418.531
Produced by FastQC (version 0.11.5)