##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631956.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	845399
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.211713049104624	33.0	31.0	34.0	30.0	34.0
2	32.39684693263181	34.0	31.0	34.0	31.0	34.0
3	32.47539919020486	34.0	31.0	34.0	30.0	34.0
4	36.04231256483625	37.0	35.0	37.0	35.0	37.0
5	35.89418369314371	37.0	35.0	37.0	35.0	37.0
6	35.9303843510579	37.0	35.0	37.0	35.0	37.0
7	35.926034925520376	37.0	35.0	37.0	35.0	37.0
8	35.89701430921967	37.0	35.0	37.0	35.0	37.0
9	37.69619907286382	39.0	37.0	39.0	35.0	39.0
10	37.579812609194	39.0	37.0	39.0	35.0	39.0
11	37.664694422397	39.0	37.0	39.0	35.0	39.0
12	37.59001016088261	39.0	37.0	39.0	35.0	39.0
13	37.65477957745396	39.0	37.0	39.0	35.0	39.0
14	38.918974354121545	40.0	38.0	41.0	36.0	41.0
15	38.90622061298866	40.0	38.0	41.0	36.0	41.0
16	38.852763014860436	40.0	38.0	41.0	36.0	41.0
17	38.815741442798014	40.0	38.0	41.0	35.0	41.0
18	38.81842419969742	40.0	38.0	41.0	35.0	41.0
19	38.83816753982439	40.0	38.0	41.0	35.0	41.0
20	38.814448562158226	40.0	38.0	41.0	35.0	41.0
21	38.79172083241168	40.0	38.0	41.0	35.0	41.0
22	38.750450379051784	40.0	38.0	41.0	35.0	41.0
23	38.69383924040601	40.0	38.0	41.0	35.0	41.0
24	38.70006115455542	40.0	38.0	41.0	35.0	41.0
25	38.633504416257885	40.0	38.0	41.0	35.0	41.0
26	38.54212862802061	40.0	38.0	41.0	34.0	41.0
27	38.43949188489695	40.0	38.0	41.0	34.0	41.0
28	38.41842017792782	40.0	38.0	41.0	34.0	41.0
29	38.3902547791043	40.0	38.0	41.0	34.0	41.0
30	38.3286377201771	40.0	38.0	41.0	34.0	41.0
31	38.264507055248465	40.0	38.0	41.0	34.0	41.0
32	38.21809110254448	40.0	38.0	41.0	34.0	41.0
33	38.142998749702805	40.0	38.0	41.0	34.0	41.0
34	38.1063592457526	40.0	38.0	41.0	33.0	41.0
35	38.00770760315543	40.0	37.0	41.0	33.0	41.0
36	37.974134107090265	40.0	37.0	41.0	33.0	41.0
37	37.92983194917429	40.0	37.0	41.0	33.0	41.0
38	37.905600787320544	40.0	37.0	41.0	33.0	41.0
39	37.839319658528105	40.0	37.0	41.0	33.0	41.0
40	37.51467295324456	40.0	37.0	41.0	33.0	41.0
41	37.583792978226846	39.0	36.0	41.0	33.0	41.0
42	37.55972032141036	39.0	36.0	41.0	33.0	41.0
43	37.03949969186148	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	1.0
16	1.0
17	4.0
18	8.0
19	15.0
20	48.0
21	102.0
22	192.0
23	402.0
24	664.0
25	1098.0
26	1790.0
27	2783.0
28	4299.0
29	6103.0
30	8311.0
31	11305.0
32	15303.0
33	20938.0
34	30469.0
35	45155.0
36	67612.0
37	118396.0
38	251893.0
39	258506.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.00123373696917	16.30981347269159	12.504154842861182	28.184797947478057
2	20.839272343591606	18.643977577451594	33.263938093137085	27.252811985819715
3	21.39628743350773	19.875467087138734	29.03232674748846	29.695918731865074
4	15.804135088875192	15.476124291606686	33.42102368230859	35.29871693720953
5	16.898056420696026	32.84993239878448	33.20349326176161	17.048517918757888
6	35.06131424333362	33.36980526355011	15.076549652885799	16.49233084023047
7	30.92965570103584	27.51079667707201	19.779417766048933	21.780129855843217
8	28.123051955348892	30.83029433439122	19.58767398589305	21.458979724366838
9	28.90765188981771	12.772667107484159	17.14799757274376	41.17168342995438
10	19.785213845769867	23.181834849579904	29.32804510059747	27.704906204052758
11	39.171444489525065	19.532670372214778	18.667398471017826	22.62848666724233
12	24.28770320286634	23.332059772959276	25.819051122606012	26.561185901568372
13	33.77825145286427	17.564723875944967	21.145636557412537	27.511388113778228
14	23.94289560314124	19.594298077002694	23.362459619658882	33.100346700197186
15	29.697693041983726	23.506651888634835	20.335368269893863	26.46028679948758
16	25.84483776299712	23.733290434457576	21.664444836106973	28.75742696643833
17	26.768070461403433	23.832888375784687	21.868845361775918	27.530195801035962
18	26.495773001860663	21.17662784081836	23.57218307568379	28.755416081637193
19	28.364476418827085	21.80544334686935	22.84518907640061	26.984891157902958
20	28.800601845992247	20.93331077988027	23.178404516683837	27.087682857443646
21	28.581770264691585	21.885169014867536	21.703834520741093	27.829226199699786
22	29.12825778123703	23.021673789536067	20.9382788482125	26.911789581014407
23	28.21803669036751	22.42976393395308	21.887416474351166	27.46478290132825
24	28.515056204230195	21.79763638234727	22.033501340787012	27.653806072635522
25	27.74453246336937	21.498369409000954	23.273862401067426	27.48323572656225
26	28.240747859886277	22.33087571667343	22.70265283020207	26.72572359323822
27	28.293622301422168	21.38741588291446	23.021555502194822	27.297406313468553
28	26.430714964176676	22.170832943970836	22.803078782917886	28.595373308934597
29	27.892628214606358	22.48228351346524	22.7816687741528	26.843419497775606
30	26.88919669883688	21.31821778828695	24.10246522647886	27.690120286397313
31	27.282028959106885	22.15959564655269	23.086022103172585	27.47235329116784
32	25.50440679489803	22.153799566831754	23.25529128849218	29.08650234977803
33	26.371453006213635	22.177102173056745	24.36660085947582	27.084843961253796
34	26.644223615121383	22.03929742050795	24.083184389856154	27.23329457451452
35	26.451888398259282	23.187394354618352	24.438401275610687	25.92231597151168
36	26.636889799964276	21.897116036333138	24.0046415952704	27.461352568432183
37	25.762509773491573	21.941000639934515	24.598798910337013	27.697690676236903
38	26.135706335115135	20.79018309697551	25.557162949092678	27.51694761881668
39	24.750324994470066	20.384812378533688	26.764521841166122	28.100340785830124
40	24.914744398798675	20.842820963828913	27.703841617981567	26.538593019390845
41	23.12529350046546	19.96560204116636	28.712477776765766	28.196626681602417
42	21.69780186633767	20.919116298931037	29.219220746653356	28.163861088077937
43	21.33099282114126	20.30934505482027	29.542145188248387	28.81751693579008
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15.0
1	11.5
2	8.0
3	21.0
4	34.0
5	34.0
6	47.0
7	60.0
8	67.0
9	74.0
10	131.0
11	188.0
12	188.0
13	398.0
14	608.0
15	1233.0
16	1858.0
17	1790.0
18	1722.0
19	1722.0
20	1839.0
21	1956.0
22	1560.5
23	1165.0
24	1236.0
25	1307.0
26	1307.0
27	1556.0
28	1805.0
29	2452.0
30	3099.0
31	4183.5
32	5268.0
33	5268.0
34	7814.5
35	10361.0
36	12239.5
37	14118.0
38	18301.5
39	22485.0
40	22485.0
41	25811.5
42	29138.0
43	35218.0
44	41298.0
45	50806.5
46	60315.0
47	60315.0
48	66401.5
49	72488.0
50	81811.0
51	91134.0
52	93178.0
53	95222.0
54	95222.0
55	88944.5
56	82667.0
57	76519.0
58	70371.0
59	64300.0
60	58229.0
61	58229.0
62	55364.0
63	52499.0
64	42169.5
65	31840.0
66	27838.5
67	23837.0
68	23837.0
69	20614.5
70	17392.0
71	16248.0
72	15104.0
73	16599.5
74	18095.0
75	18095.0
76	15351.0
77	12607.0
78	8367.0
79	4127.0
80	2716.5
81	1306.0
82	1306.0
83	1016.5
84	727.0
85	588.5
86	450.0
87	351.0
88	252.0
89	252.0
90	192.5
91	133.0
92	81.5
93	30.0
94	18.0
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	845399.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.37803829885239
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.93013334884246	32.210738720896636
2	7.032318797775022	5.397732003014986
3	2.424105524463882	2.790972439750035
4	1.331177533281127	2.0435192941933975
5	0.8638791157406157	1.657699289473604
6	0.603920867041018	1.3906378898865794
7	0.4488238448878389	1.2057485095980565
8	0.35704119169503296	1.0962032423311896
9	0.2763505011265378	0.9545211104527184
>10	2.164838895457455	17.053028358889932
>50	0.28603446987024783	7.6819445528079555
>100	0.26025718811053117	20.03710779900572
>500	0.016149610718075705	4.037338364616716
>1k	0.0049691109901771405	2.442808425082502
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1980	0.23420893566233222	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1606	0.18996947003722503	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	1568	0.18547455106996813	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1514	0.17908703464281364	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1327	0.15696730183026003	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1256	0.1485689006019643	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1243	0.14703116516579745	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1189	0.14064364873864293	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1189	0.14064364873864293	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1184	0.14005221203242493	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1125	0.1330732588990524	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1124	0.1329549715578088	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1070	0.1265674551306543	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1069	0.12644916778941068	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1041	0.12313712223458982	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	1010	0.11947021465603816	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	939	0.11107181342774239	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	930	0.11000722735654998	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	915	0.10823291723789595	No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC	872	0.10314656156442106	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	869	0.10279169954069026	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	863	0.10208197549322864	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1828734124360213E-4	0.0	0.0	0.0
3	0.0	1.1828734124360213E-4	0.0	0.0	0.0
4	0.0	1.1828734124360213E-4	0.0	0.0	0.0
5	1.1828734124360213E-4	1.1828734124360213E-4	0.0	0.0	0.0
6	1.1828734124360213E-4	1.1828734124360213E-4	0.0	0.0	0.0
7	1.1828734124360213E-4	1.1828734124360213E-4	0.0	0.0	0.0
8	1.1828734124360213E-4	1.1828734124360213E-4	0.0	0.0	0.0
9	1.1828734124360213E-4	1.1828734124360213E-4	0.0	0.0	0.0
10	1.1828734124360213E-4	1.1828734124360213E-4	0.0	0.0	0.0
11	1.1828734124360213E-4	1.1828734124360213E-4	1.1828734124360213E-4	0.0	0.0
12	1.1828734124360213E-4	1.1828734124360213E-4	1.1828734124360213E-4	0.0	0.0
13	1.1828734124360213E-4	1.1828734124360213E-4	1.1828734124360213E-4	2.3657468248720426E-4	0.0
14	1.1828734124360213E-4	1.1828734124360213E-4	1.1828734124360213E-4	2.3657468248720426E-4	0.0
15	2.3657468248720426E-4	1.1828734124360213E-4	1.1828734124360213E-4	4.731493649744085E-4	0.0
16	2.3657468248720426E-4	1.1828734124360213E-4	1.1828734124360213E-4	0.0010645860711924191	0.0
17	2.3657468248720426E-4	1.1828734124360213E-4	2.3657468248720426E-4	0.00165602277741043	0.0
18	3.548620237308064E-4	1.1828734124360213E-4	2.3657468248720426E-4	0.00165602277741043	0.0
19	3.548620237308064E-4	1.1828734124360213E-4	2.3657468248720426E-4	0.0027206088486028492	0.0
20	4.731493649744085E-4	1.1828734124360213E-4	2.3657468248720426E-4	0.0031937582135772575	0.0
21	5.914367062180107E-4	1.1828734124360213E-4	2.3657468248720426E-4	0.005086355673474892	0.0
22	5.914367062180107E-4	1.1828734124360213E-4	2.3657468248720426E-4	0.008043539204564945	0.0
23	5.914367062180107E-4	1.1828734124360213E-4	2.3657468248720426E-4	0.010290998688193385	0.0
24	5.914367062180107E-4	1.1828734124360213E-4	2.3657468248720426E-4	0.012301883489334623	0.0
25	5.914367062180107E-4	1.1828734124360213E-4	2.3657468248720426E-4	0.013721331584257847	0.0
26	5.914367062180107E-4	1.1828734124360213E-4	2.3657468248720426E-4	0.017388239162809512	0.0
27	5.914367062180107E-4	1.1828734124360213E-4	2.3657468248720426E-4	0.039153109951632306	0.0
28	5.914367062180107E-4	1.1828734124360213E-4	2.3657468248720426E-4	0.14679459048331026	0.0
29	5.914367062180107E-4	1.1828734124360213E-4	2.3657468248720426E-4	0.31428946568425087	0.0
30	5.914367062180107E-4	1.1828734124360213E-4	2.3657468248720426E-4	0.5288627027001451	0.0
31	5.914367062180107E-4	1.1828734124360213E-4	2.3657468248720426E-4	1.1146216165384628	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACTAG	25	0.005495532	29.599998	16
GGTATCA	1075	0.0	28.223255	1
AAGAGCG	35	8.867447E-4	26.42857	5
ATACACA	130	0.0	25.615385	37
TTACACC	45	1.3228077E-4	24.666666	4
CTATATC	105	9.822543E-10	21.142859	4
TCGCTTA	70	5.0992894E-6	21.142857	23
GTACTAT	80	6.9556336E-7	20.8125	1
CTTATAC	930	0.0	19.892473	37
CCGCTTA	85	1.2445107E-6	19.588236	25
CTTAGTC	85	1.2445107E-6	19.588236	18
TATACAC	275	0.0	19.509092	37
CCAGGAC	545	0.0	19.009174	3
GACAGGC	495	0.0	18.686869	7
CATCAGA	495	0.0	18.686869	16
GCGGTAA	515	0.0	18.679611	23
CAACCAA	70	1.2187132E-4	18.5	10
TGCGGGT	440	0.0	18.5	21
TACCTTA	80	1.6158467E-5	18.5	31
TCTATCG	70	1.2187132E-4	18.5	31
>>END_MODULE