FastQCFastQC Report
Fri 10 Feb 2017
ERR1631955.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631955.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510858
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA17940.3511739074263298No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA17650.34549718317027434No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA17480.3421694482615521No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA17150.33570972755638556No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC15300.299496141784997No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA14220.2783552376589972No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG14000.27404875718888616No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13560.26543579624866404No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12940.2532993512874419No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12190.23861816786660872No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC11590.22687322112994218No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT11470.22452423178260886No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA11180.21884750752655338No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9850.19281287559360918No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA9180.1796976850709982No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8930.17480395726405382No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA8310.16266751230283172No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8280.16208026496599837No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA8260.1616887667414428No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8140.1593397773941095No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT7940.155424795148554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7860.1538588022503318No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT7610.1489650744433874No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7590.14857357621883185No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA7570.1481820779942763No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC7470.14622458687149853No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7420.14524584131010965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7410.14505009219783188No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA7380.14446284486099856No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT7240.1417223572891097No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA7200.14093936083999858No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7080.1385903714926653No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6970.13643713125760976No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC6880.13467538924710976No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG6820.1335008945734431No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT6780.13271789812433202No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6720.13154340345066534No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6720.13154340345066534No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT6660.1303689087769987No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC6480.12684542475599872No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6470.12664967564372095No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC6360.12449643540866542No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC6290.123126191622721No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA6240.12214744606133211No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC6190.12116870049994324No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG6190.12116870049994324No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA6020.11784096559122105No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5930.11607922358072106No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5920.11588347446844328No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA5850.11451323068249884No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA5820.11392598334566553No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5800.11353448512110997No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG5760.11275148867199887No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC5740.11235999044744331No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC5630.11020675021238778No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC5480.10727051352822115No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA5370.10511727329316561No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC5330.10433427684405452No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT5290.1035512803949434No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5280.10335553128266563No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC5170.1012022910476101No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG5120.10022354548622121No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTAGC200.00184097937.031
TATAAAG303.5967032E-430.8333344
GGTATCA10550.029.8104271
CATCTAC250.00549352129.61
GTATAAG250.00549352129.612
TATACAA358.862622E-426.4285725
TAGCGAA856.91216E-1126.11764510
TACCGCT509.078185E-625.90000235
GCAGTCG2450.024.9183679
GCCTAGT451.3218068E-424.6666661
CAGTCGG2500.024.4210
AACGACC1450.024.2413837
CTCCACT701.9185791E-723.7857152
TTTTACG701.9185791E-723.7857154
ATACGGC1200.023.12500229
GTTTATA400.001929726623.1251
ATTGTTA802.7182978E-823.12514
TTTTTAC802.7182978E-823.1253
TCAATGC400.001929726623.12532
TTGAAAG400.001929726623.1252
Produced by FastQC (version 0.11.5)