##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631955.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	510858
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55788105500942	34.0	31.0	34.0	31.0	34.0
2	32.72522697109569	34.0	31.0	34.0	31.0	34.0
3	32.81278359152641	34.0	31.0	34.0	31.0	34.0
4	36.24729376852276	37.0	37.0	37.0	35.0	37.0
5	36.16968707546911	37.0	35.0	37.0	35.0	37.0
6	36.22433827012595	37.0	36.0	37.0	35.0	37.0
7	36.23346409374034	37.0	36.0	37.0	35.0	37.0
8	36.20960814942704	37.0	36.0	37.0	35.0	37.0
9	38.05064616781963	39.0	38.0	39.0	35.0	39.0
10	37.9770542890588	39.0	38.0	39.0	35.0	39.0
11	38.05204773146354	39.0	38.0	39.0	35.0	39.0
12	37.976050096112814	39.0	38.0	39.0	35.0	39.0
13	38.04306480470111	39.0	38.0	39.0	35.0	39.0
14	39.375016149301764	40.0	39.0	41.0	37.0	41.0
15	39.40733824272107	40.0	39.0	41.0	37.0	41.0
16	39.32343625821657	40.0	39.0	41.0	36.0	41.0
17	39.31010770116158	40.0	39.0	41.0	36.0	41.0
18	39.35280645502273	40.0	39.0	41.0	36.0	41.0
19	39.364686468646866	40.0	39.0	41.0	36.0	41.0
20	39.372919676309266	40.0	39.0	41.0	37.0	41.0
21	39.327182896225565	40.0	39.0	41.0	36.0	41.0
22	39.30053752706232	40.0	39.0	41.0	36.0	41.0
23	39.24475098755427	40.0	39.0	41.0	36.0	41.0
24	39.24975041988185	40.0	39.0	41.0	36.0	41.0
25	39.20168618285316	40.0	39.0	41.0	36.0	41.0
26	39.12963093462371	40.0	39.0	41.0	36.0	41.0
27	38.98547541586899	40.0	39.0	41.0	35.0	41.0
28	38.99692478144612	40.0	39.0	41.0	35.0	41.0
29	38.980667817671446	40.0	38.0	41.0	35.0	41.0
30	38.942457590954824	40.0	38.0	41.0	35.0	41.0
31	38.846704955193026	40.0	38.0	41.0	35.0	41.0
32	38.81263873718333	40.0	38.0	41.0	35.0	41.0
33	38.73750631290887	40.0	38.0	41.0	35.0	41.0
34	38.71492665280763	40.0	38.0	41.0	35.0	41.0
35	38.64404394176072	40.0	38.0	41.0	35.0	41.0
36	38.59398306378681	40.0	38.0	41.0	35.0	41.0
37	38.541440087069205	40.0	38.0	41.0	35.0	41.0
38	38.508272357484856	40.0	38.0	41.0	35.0	41.0
39	38.413821453319706	40.0	38.0	41.0	34.0	41.0
40	38.121137380641976	40.0	37.0	41.0	34.0	41.0
41	38.17650501704975	40.0	37.0	41.0	34.0	41.0
42	38.15537194288824	40.0	37.0	41.0	34.0	41.0
43	37.67447509875543	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	0.0
17	2.0
18	4.0
19	7.0
20	18.0
21	30.0
22	63.0
23	127.0
24	237.0
25	413.0
26	680.0
27	1106.0
28	1705.0
29	2414.0
30	3631.0
31	4891.0
32	6650.0
33	9164.0
34	13358.0
35	20152.0
36	30258.0
37	55421.0
38	127115.0
39	233410.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.36421862826852	16.04692497719523	13.3228803307377	28.265976063798547
2	20.93321431787307	19.78357978146569	32.48593542628284	26.797270474378397
3	21.321776305744454	19.589396662086138	30.241280355793588	28.847546676375824
4	14.983028551965516	16.950894377694	35.94932446981353	32.11675260052695
5	16.419827036084392	32.76174592548223	33.87301363588316	16.94541340255022
6	30.8277446961778	35.14303387634137	16.986716465240832	17.042504962239995
7	28.71933100783388	28.115836494681496	20.45284599634341	22.71198650114122
8	26.567852514788846	30.02908831808448	21.517916916246786	21.88514225087989
9	28.697602856371045	12.511304511234043	17.883834646809877	40.907257985585034
10	19.596835128352694	22.750353327147664	28.837954969874215	28.814856574625438
11	39.189363776235275	19.64107442772747	18.7547224473337	22.414839348703552
12	21.700746587114228	24.36352176142881	27.173696017288563	26.762035634168402
13	36.12921790399681	17.756206225604767	20.628237200944294	25.486338669454135
14	23.568976114693317	21.19532237921301	24.117856625520204	31.117844880573465
15	30.54547447627325	23.96438932149443	20.01241049371841	25.47772570851391
16	23.713634708666596	24.71195518128325	23.479323021270098	28.09508708878005
17	26.672382540745176	24.09710721961876	21.532010852330785	27.69849938730528
18	26.6281432413704	20.54935030869635	23.55214169103743	29.270364758895816
19	27.969220409585443	22.520348120221275	23.762180488511483	25.748250981681796
20	30.883141694952414	19.938221580165134	22.88874794952805	26.289888775354402
21	27.354568197033224	21.881618766858892	22.06307819394039	28.700734842167492
22	28.607166766498715	22.940425715169383	21.350551425249286	27.101856093082617
23	28.11838123314111	21.721887491240228	22.52602484447733	27.633706431141334
24	27.57087096610017	20.892694251631568	23.99590492857115	27.540529853697116
25	27.74215143934322	22.06072920459306	23.66822091461815	26.52889844144557
26	27.914410658147666	22.89325017911044	22.59394978643772	26.598389376304176
27	26.457058517239624	21.540819562383284	25.502977343997745	26.499144576379347
28	25.843189301136523	23.146158032173325	22.112798468458948	28.897854198231208
29	27.195815666975946	23.82579895000176	22.322250018596165	26.65613536442612
30	26.561392794083677	21.827396262757947	24.422246495112145	27.188964448046228
31	28.137760395256606	22.334582212669666	23.16514569606427	26.36251169600946
32	25.242631024668306	22.231813928723835	23.257735026171655	29.267820020436208
33	25.412541254125415	21.673537460507617	25.544280406688358	27.369640878678613
34	25.609660610189135	22.128066899216613	24.394841619393258	27.867430871200998
35	24.942351886434196	24.281111385159868	25.216596392735358	25.55994033567058
36	25.957898280931296	22.517411883537108	25.488883407913743	26.035806427617853
37	26.939580079004344	22.485113280011277	24.553789898562812	26.021516742421575
38	25.695398721366796	21.674516206069004	25.574230020866857	27.055855051697343
39	25.190953259026973	20.197001906596356	26.803142947746732	27.808901886629943
40	24.952922338497192	21.523202142278286	27.620591240618726	25.9032842786058
41	22.899709899815605	20.626475458933793	28.76689804211738	27.706916599133223
42	21.053012774587067	20.963359681163844	30.278668436238643	27.704959108010446
43	20.00301453632908	22.47336833327461	29.44360272326165	28.080014407134662
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	4.0
2	5.0
3	16.0
4	27.0
5	27.0
6	37.0
7	47.0
8	57.5
9	68.0
10	100.5
11	133.0
12	133.0
13	293.0
14	453.0
15	889.0
16	1325.0
17	1274.0
18	1223.0
19	1223.0
20	1340.0
21	1457.0
22	1172.5
23	888.0
24	914.5
25	941.0
26	941.0
27	1051.0
28	1161.0
29	1580.0
30	1999.0
31	2167.0
32	2335.0
33	2335.0
34	2935.5
35	3536.0
36	3924.5
37	4313.0
38	6015.5
39	7718.0
40	7718.0
41	10360.5
42	13003.0
43	15969.0
44	18935.0
45	31331.5
46	43728.0
47	43728.0
48	50173.0
49	56618.0
50	63616.0
51	70614.0
52	70314.5
53	70015.0
54	70015.0
55	61753.5
56	53492.0
57	50040.0
58	46588.0
59	41945.5
60	37303.0
61	37303.0
62	32725.5
63	28148.0
64	21933.0
65	15718.0
66	13377.5
67	11037.0
68	11037.0
69	9170.5
70	7304.0
71	6027.5
72	4751.0
73	3584.0
74	2417.0
75	2417.0
76	1872.5
77	1328.0
78	1111.0
79	894.0
80	737.5
81	581.0
82	581.0
83	478.5
84	376.0
85	278.0
86	180.0
87	152.0
88	124.0
89	124.0
90	88.0
91	52.0
92	35.5
93	19.0
94	10.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	510858.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.93679300479081
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.71997927061389	27.733140208626196
2	7.621343702963447	5.172879273316727
3	2.857163362282258	2.9088888481993536
4	1.5387709724468028	2.088838078948312
5	0.9925449118201248	1.684189561019896
6	0.6928672423794677	1.4108215310659327
7	0.5013550388532586	1.191006752283032
8	0.4450097625300976	1.2081763356876025
9	0.3374298617588896	1.0306158634931952
>10	2.6110067101642644	17.98701191649508
>50	0.37054896849263624	8.7219843225012
>100	0.2748673099616738	18.456285597956963
>500	0.029574330818661107	6.732576842443333
>1k	0.007538554914560674	3.673584867963219
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1794	0.3511739074263298	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1765	0.34549718317027434	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1748	0.3421694482615521	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1715	0.33570972755638556	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1530	0.299496141784997	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1422	0.2783552376589972	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1400	0.27404875718888616	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1356	0.26543579624866404	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1294	0.2532993512874419	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1219	0.23861816786660872	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1159	0.22687322112994218	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1147	0.22452423178260886	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1118	0.21884750752655338	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	985	0.19281287559360918	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	918	0.1796976850709982	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	893	0.17480395726405382	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	831	0.16266751230283172	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	828	0.16208026496599837	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	826	0.1616887667414428	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	814	0.1593397773941095	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	794	0.155424795148554	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	786	0.1538588022503318	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	761	0.1489650744433874	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	759	0.14857357621883185	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	757	0.1481820779942763	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	747	0.14622458687149853	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	742	0.14524584131010965	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	741	0.14505009219783188	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	738	0.14446284486099856	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	724	0.1417223572891097	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	720	0.14093936083999858	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	708	0.1385903714926653	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	697	0.13643713125760976	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	688	0.13467538924710976	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	682	0.1335008945734431	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	678	0.13271789812433202	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	672	0.13154340345066534	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	672	0.13154340345066534	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	666	0.1303689087769987	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	648	0.12684542475599872	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	647	0.12664967564372095	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	636	0.12449643540866542	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	629	0.123126191622721	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	624	0.12214744606133211	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	619	0.12116870049994324	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	619	0.12116870049994324	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	602	0.11784096559122105	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	593	0.11607922358072106	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	592	0.11588347446844328	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	585	0.11451323068249884	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	582	0.11392598334566553	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	580	0.11353448512110997	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	576	0.11275148867199887	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	574	0.11235999044744331	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	563	0.11020675021238778	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	548	0.10727051352822115	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	537	0.10511727329316561	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	533	0.10433427684405452	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	529	0.1035512803949434	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	528	0.10335553128266563	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	517	0.1012022910476101	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	512	0.10022354548622121	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	5.872473368333274E-4	0.0	0.0	0.0	0.0
9	7.829964491111032E-4	0.0	0.0	0.0	0.0
10	7.829964491111032E-4	0.0	0.0	0.0	0.0
11	7.829964491111032E-4	0.0	0.0	0.0	0.0
12	7.829964491111032E-4	0.0	0.0	0.0	0.0
13	7.829964491111032E-4	0.0	0.0	0.0	0.0
14	7.829964491111032E-4	0.0	0.0	0.0	0.0
15	7.829964491111032E-4	0.0	0.0	1.957491122777758E-4	0.0
16	7.829964491111032E-4	0.0	0.0	7.829964491111032E-4	0.0
17	9.78745561388879E-4	0.0	0.0	0.0013702437859444308	0.0
18	9.78745561388879E-4	0.0	0.0	0.0017617420104999823	0.0
19	9.78745561388879E-4	0.0	0.0	0.002153240235055534	0.0
20	9.78745561388879E-4	0.0	0.0	0.0037192331332777406	0.0
21	9.78745561388879E-4	0.0	0.0	0.006655469817444378	0.0
22	9.78745561388879E-4	0.0	0.0	0.009983204726166567	0.0
23	9.78745561388879E-4	0.0	0.0	0.012332194073499878	0.0
24	9.78745561388879E-4	0.0	0.0	0.015659928982222064	0.0
25	9.78745561388879E-4	0.0	0.0	0.017421670992722047	0.0
26	9.78745561388879E-4	0.0	0.0	0.022315398799666444	0.0
27	9.78745561388879E-4	0.0	0.0	0.04306480470111068	0.0
28	9.78745561388879E-4	0.0	0.0	0.14191810640138747	0.0
29	9.78745561388879E-4	0.0	0.0	0.3149603216549413	0.0
30	9.78745561388879E-4	0.0	0.0	0.5461400232549946	0.0
31	9.78745561388879E-4	0.0	0.0	1.1363235967724887	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTTAGC	20	0.001840979	37.0	31
TATAAAG	30	3.5967032E-4	30.833334	4
GGTATCA	1055	0.0	29.810427	1
CATCTAC	25	0.005493521	29.6	1
GTATAAG	25	0.005493521	29.6	12
TATACAA	35	8.862622E-4	26.428572	5
TAGCGAA	85	6.91216E-11	26.117645	10
TACCGCT	50	9.078185E-6	25.900002	35
GCAGTCG	245	0.0	24.918367	9
GCCTAGT	45	1.3218068E-4	24.666666	1
CAGTCGG	250	0.0	24.42	10
AACGACC	145	0.0	24.24138	37
CTCCACT	70	1.9185791E-7	23.785715	2
TTTTACG	70	1.9185791E-7	23.785715	4
ATACGGC	120	0.0	23.125002	29
GTTTATA	40	0.0019297266	23.125	1
ATTGTTA	80	2.7182978E-8	23.125	14
TTTTTAC	80	2.7182978E-8	23.125	3
TCAATGC	40	0.0019297266	23.125	32
TTGAAAG	40	0.0019297266	23.125	2
>>END_MODULE