FastQCFastQC Report
Fri 10 Feb 2017
ERR1631954.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631954.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482022
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11090.23007248631805188No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10860.22530091987502643No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10520.2182472999157715No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10320.214098111704445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10070.20891162644028696No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9710.20144308765989932No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9130.18941044184705264No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8890.18443141599346088No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8630.17903747131873649No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8370.1736435266440121No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7570.1570467737987063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7520.15600947674587468No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7460.15476472028247673No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6980.14480666857529326No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6680.13858288625830356No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6590.13671575156320664No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5650.11721456696997233No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5650.11721456696997233No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5530.11472505404317646No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5520.11451759463261013No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5360.11119824406354897No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5120.10621921820995722No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT5060.10497446174655928No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA5000.10372970528316133No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4900.10165511117749813No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4870.10103273294579916No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGT200.001840876337.02
GCACTTA250.00549321629.66
GGTATCA8400.027.9702381
CTAAAGG358.861895E-426.428573
ATACGGC751.3695171E-824.66666429
GACCGCT400.001929569823.12519
TCACGAT502.698949E-422.220
AACGACC855.1650204E-821.76470637
CTTATTG1650.021.3030328
AGGACCG806.9428825E-720.81255
TAGCAAG450.003822633320.55555712
GGCCAGA450.003822633320.5555571
CCGCATA450.003822633320.55555723
CTTATAC5150.020.47572737
GTCACGA555.137141E-420.18181819
TACGGCT951.6710692E-719.47368430
CGCGGTA4950.019.43434322
GCAGTCG2000.019.4250019
GGTAATA5000.019.24000225
GCGGTAA5000.019.24000223
Produced by FastQC (version 0.11.5)