##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631954.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	482022
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.570507155275074	34.0	31.0	34.0	31.0	34.0
2	32.73748708565169	34.0	31.0	34.0	31.0	34.0
3	32.83017372651041	34.0	31.0	34.0	31.0	34.0
4	36.26010223599753	37.0	37.0	37.0	35.0	37.0
5	36.18324889735323	37.0	35.0	37.0	35.0	37.0
6	36.23663857666248	37.0	37.0	37.0	35.0	37.0
7	36.230974934754016	37.0	37.0	37.0	35.0	37.0
8	36.21383048906481	37.0	36.0	37.0	35.0	37.0
9	38.03947330204846	39.0	38.0	39.0	35.0	39.0
10	37.975034334532445	39.0	38.0	39.0	35.0	39.0
11	38.05064499130745	39.0	38.0	39.0	35.0	39.0
12	37.98378704706424	39.0	38.0	39.0	35.0	39.0
13	38.03564982511172	39.0	38.0	39.0	35.0	39.0
14	39.39982614901395	40.0	39.0	41.0	37.0	41.0
15	39.409157673301216	40.0	39.0	41.0	37.0	41.0
16	39.35150885229305	40.0	39.0	41.0	36.0	41.0
17	39.329175846745585	40.0	39.0	41.0	36.0	41.0
18	39.36737119882495	40.0	39.0	41.0	36.0	41.0
19	39.38672301264258	40.0	39.0	41.0	37.0	41.0
20	39.388700100825275	40.0	39.0	41.0	37.0	41.0
21	39.348108592553864	40.0	39.0	41.0	36.0	41.0
22	39.32518017849808	40.0	39.0	41.0	36.0	41.0
23	39.26494226404604	40.0	39.0	41.0	36.0	41.0
24	39.276441739173734	40.0	39.0	41.0	36.0	41.0
25	39.225394276609784	40.0	39.0	41.0	36.0	41.0
26	39.168566994867454	40.0	39.0	41.0	36.0	41.0
27	39.04799988382273	40.0	39.0	41.0	35.0	41.0
28	39.05433361962732	40.0	39.0	41.0	35.0	41.0
29	39.03955628581268	40.0	39.0	41.0	35.0	41.0
30	38.99795652480592	40.0	38.0	41.0	35.0	41.0
31	38.91111816473107	40.0	38.0	41.0	35.0	41.0
32	38.8610167170793	40.0	38.0	41.0	35.0	41.0
33	38.80717892544324	40.0	38.0	41.0	35.0	41.0
34	38.78243109235678	40.0	38.0	41.0	35.0	41.0
35	38.7141935430333	40.0	38.0	41.0	35.0	41.0
36	38.672969283559674	40.0	38.0	41.0	35.0	41.0
37	38.62000282144798	40.0	38.0	41.0	35.0	41.0
38	38.5786125944459	40.0	38.0	41.0	35.0	41.0
39	38.510474625639496	40.0	38.0	41.0	35.0	41.0
40	38.21422051275668	40.0	38.0	41.0	34.0	41.0
41	38.26332408064362	40.0	38.0	41.0	34.0	41.0
42	38.25466265025248	40.0	37.0	41.0	34.0	41.0
43	37.78274020687852	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	5.0
19	8.0
20	17.0
21	41.0
22	64.0
23	133.0
24	227.0
25	384.0
26	630.0
27	1009.0
28	1565.0
29	2187.0
30	3256.0
31	4608.0
32	6065.0
33	8568.0
34	12427.0
35	18200.0
36	28136.0
37	51219.0
38	115546.0
39	227726.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.17068930463755	17.075361705482322	13.465775421038872	27.288173568841255
2	19.984150101032732	20.39388243690122	33.785387388957346	25.836580073108696
3	20.97207181414956	20.624784760861537	30.356290791706602	28.0468526332823
4	15.055744343619171	16.978893079568984	34.99342353668505	32.9719390401268
5	16.401948458784037	33.50573210351395	33.598673919447656	16.493645518254354
6	31.883814431706437	34.63846048520607	16.599864736464312	16.877860346623184
7	28.639149250449147	28.8692217367672	20.92975009439403	21.561878918389617
8	26.284484940521384	31.682163884635973	20.97456132707636	21.058789847766285
9	27.92777093161723	13.764102053433247	18.68566165029812	39.622465364651404
10	18.84291588350739	24.416727867192783	30.211691582541878	26.52866466675795
11	37.487293111102815	20.656526050678185	19.896809689184312	21.95937114903469
12	21.801701997004287	24.963383414035047	27.725498006315064	25.509416582645606
13	34.149063735680116	18.503719747231454	22.000448112326822	25.34676840476161
14	23.349349199829053	21.39694868698939	24.32897253652323	30.92472957665833
15	28.695163291302055	25.49779885565389	20.95568252071482	24.85135533232923
16	24.061972275124372	25.34095954126575	23.755969644539046	26.841098539070828
17	25.772682574654272	24.94989855234823	22.899162278900135	26.378256594097365
18	25.592815265693268	22.219110331063728	24.720863363083012	27.467211040159995
19	26.900639389903365	23.610333138321487	24.902390347328545	24.586637124446604
20	28.651389355672563	21.90937343108821	24.27752260270278	25.16171461053645
21	26.116235358552096	23.479011331433007	23.348726821597353	27.05602648841754
22	26.926156897403025	23.935836953500047	23.020318574670863	26.117687574426064
23	26.399209994564565	23.082971316661897	24.004920937218632	26.51289775155491
24	26.57285352120858	22.84584521038459	24.51340395251669	26.06789731589015
25	26.598371028708236	23.242507603387395	24.63642738298252	25.52269398492185
26	26.52410055972549	23.879615453236575	23.887913829659226	25.708370157378706
27	25.76874084585351	22.84916456095365	25.63721157955446	25.744883013638383
28	25.13578218421566	24.1455784175826	23.59207671019165	27.126562688010093
29	25.90400438154275	24.506350332557435	23.73024467762882	25.859400608270995
30	25.311500304965335	23.61074805714262	25.236192538929757	25.841559098962286
31	26.876989017098808	23.3016335353988	24.222753318313273	25.598624129189123
32	24.25947363398351	23.318852666475802	24.442037915281876	27.97963578425881
33	24.607175606092667	22.96928355967155	26.026197974366315	26.397342859869465
34	24.843056955906576	23.04791067627619	25.393446772139033	26.715585595678203
35	24.713809743123758	24.393907332030487	25.679740758720556	25.212542166125196
36	25.008609565538503	23.19168004779865	26.201086257473726	25.598624129189123
37	25.792183759247507	22.636518665123166	25.588666077481943	25.982631498147384
38	24.656758405218017	22.351884353826172	26.61164843098448	26.37970880997133
39	24.371086796868195	21.064391251851575	27.873416566048853	26.691105385231378
40	23.97359456622312	22.04754139852538	28.367169963196698	25.611694072054803
41	22.350639597362775	21.517067685707293	29.42438311944268	26.70790959748725
42	21.375787827111626	21.79029172942314	30.039915190592957	26.794005252872278
43	20.296376513935048	22.970735775545513	29.625618747692013	27.107268962827426
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14.0
1	11.5
2	9.0
3	30.0
4	51.0
5	51.0
6	68.5
7	86.0
8	101.5
9	117.0
10	174.5
11	232.0
12	232.0
13	431.5
14	631.0
15	1320.0
16	2009.0
17	1908.5
18	1808.0
19	1808.0
20	2090.5
21	2373.0
22	2121.0
23	1869.0
24	2034.0
25	2199.0
26	2199.0
27	2637.0
28	3075.0
29	3910.5
30	4746.0
31	5394.0
32	6042.0
33	6042.0
34	7045.0
35	8048.0
36	8788.0
37	9528.0
38	11945.0
39	14362.0
40	14362.0
41	16943.0
42	19524.0
43	21485.0
44	23446.0
45	31563.5
46	39681.0
47	39681.0
48	43991.0
49	48301.0
50	53613.5
51	58926.0
52	58824.5
53	58723.0
54	58723.0
55	51331.5
56	43940.0
57	40622.5
58	37305.0
59	33386.0
60	29467.0
61	29467.0
62	26238.5
63	23010.0
64	18492.0
65	13974.0
66	11814.5
67	9655.0
68	9655.0
69	8208.0
70	6761.0
71	5695.0
72	4629.0
73	3730.5
74	2832.0
75	2832.0
76	2242.5
77	1653.0
78	1365.0
79	1077.0
80	894.0
81	711.0
82	711.0
83	629.5
84	548.0
85	437.5
86	327.0
87	266.0
88	205.0
89	205.0
90	155.5
91	106.0
92	61.5
93	17.0
94	11.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	482022.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.95878353658334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.52754925498232	39.30742122624202
2	6.426547493222527	5.907126102571728
3	2.2528237284107324	3.1061111424032237
4	1.2104073091204288	2.2251539004385643
5	0.7940592095006745	1.8246997662335989
6	0.5313709792040358	1.4652698286516364
7	0.4125716676616858	1.327290437717343
8	0.2969332095897042	1.0917351283484917
9	0.25129849614471234	1.0394435868645377
>10	1.894843742720029	17.7794572666727
>50	0.23778048875512542	7.634238592524352
>100	0.15337749083813776	13.573995718499948
>500	0.008168032056468875	2.6156537296453415
>1k	0.0022688977934635763	1.1024035731865154
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1109	0.23007248631805188	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1086	0.22530091987502643	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1052	0.2182472999157715	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1032	0.214098111704445	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1007	0.20891162644028696	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	971	0.20144308765989932	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	913	0.18941044184705264	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	889	0.18443141599346088	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	863	0.17903747131873649	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	837	0.1736435266440121	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	757	0.1570467737987063	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	752	0.15600947674587468	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	746	0.15476472028247673	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	698	0.14480666857529326	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	668	0.13858288625830356	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	659	0.13671575156320664	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	565	0.11721456696997233	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	565	0.11721456696997233	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	553	0.11472505404317646	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	552	0.11451759463261013	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	536	0.11119824406354897	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	512	0.10621921820995722	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	506	0.10497446174655928	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	500	0.10372970528316133	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	490	0.10165511117749813	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	487	0.10103273294579916	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	2.074594105663227E-4	0.0	0.0
3	0.0	0.0	2.074594105663227E-4	0.0	0.0
4	0.0	0.0	2.074594105663227E-4	0.0	0.0
5	0.0	0.0	2.074594105663227E-4	0.0	0.0
6	0.0	0.0	2.074594105663227E-4	0.0	0.0
7	0.0	0.0	2.074594105663227E-4	0.0	0.0
8	0.0	0.0	2.074594105663227E-4	0.0	0.0
9	0.0	0.0	2.074594105663227E-4	0.0	0.0
10	0.0	0.0	2.074594105663227E-4	0.0	0.0
11	0.0	0.0	2.074594105663227E-4	0.0	0.0
12	0.0	0.0	2.074594105663227E-4	2.074594105663227E-4	0.0
13	0.0	0.0	2.074594105663227E-4	2.074594105663227E-4	0.0
14	0.0	0.0	2.074594105663227E-4	4.149188211326454E-4	2.074594105663227E-4
15	0.0	0.0	2.074594105663227E-4	4.149188211326454E-4	2.074594105663227E-4
16	0.0	0.0	2.074594105663227E-4	0.0014522158739642588	2.074594105663227E-4
17	0.0	0.0	2.074594105663227E-4	0.0024895129267958724	2.074594105663227E-4
18	0.0	0.0	2.074594105663227E-4	0.0029044317479285176	2.074594105663227E-4
19	0.0	0.0	2.074594105663227E-4	0.0037342693901938084	2.074594105663227E-4
20	0.0	0.0	2.074594105663227E-4	0.004979025853591745	2.074594105663227E-4
21	4.149188211326454E-4	0.0	2.074594105663227E-4	0.007053619959254972	2.074594105663227E-4
22	4.149188211326454E-4	0.0	2.074594105663227E-4	0.011202808170581426	2.074594105663227E-4
23	4.149188211326454E-4	0.0	2.074594105663227E-4	0.012655024044545684	2.074594105663227E-4
24	4.149188211326454E-4	0.0	2.074594105663227E-4	0.01618183402417317	2.074594105663227E-4
25	4.149188211326454E-4	0.0	2.074594105663227E-4	0.01846388754040272	2.074594105663227E-4
26	4.149188211326454E-4	0.0	2.074594105663227E-4	0.021990697520030206	2.074594105663227E-4
27	4.149188211326454E-4	0.0	2.074594105663227E-4	0.045018692092892026	2.074594105663227E-4
28	4.149188211326454E-4	0.0	2.074594105663227E-4	0.14605142503869117	2.074594105663227E-4
29	4.149188211326454E-4	0.0	2.074594105663227E-4	0.30869960292268817	2.074594105663227E-4
30	4.149188211326454E-4	0.0	2.074594105663227E-4	0.5093128529403222	2.074594105663227E-4
31	4.149188211326454E-4	0.0	2.074594105663227E-4	1.0897842837048932	2.074594105663227E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGGT	20	0.0018408763	37.0	2
GCACTTA	25	0.005493216	29.6	6
GGTATCA	840	0.0	27.970238	1
CTAAAGG	35	8.861895E-4	26.42857	3
ATACGGC	75	1.3695171E-8	24.666664	29
GACCGCT	40	0.0019295698	23.125	19
TCACGAT	50	2.698949E-4	22.2	20
AACGACC	85	5.1650204E-8	21.764706	37
CTTATTG	165	0.0	21.30303	28
AGGACCG	80	6.9428825E-7	20.8125	5
TAGCAAG	45	0.0038226333	20.555557	12
GGCCAGA	45	0.0038226333	20.555557	1
CCGCATA	45	0.0038226333	20.555557	23
CTTATAC	515	0.0	20.475727	37
GTCACGA	55	5.137141E-4	20.181818	19
TACGGCT	95	1.6710692E-7	19.473684	30
CGCGGTA	495	0.0	19.434343	22
GCAGTCG	200	0.0	19.425001	9
GGTAATA	500	0.0	19.240002	25
GCGGTAA	500	0.0	19.240002	23
>>END_MODULE