FastQCFastQC Report
Fri 10 Feb 2017
ERR1631953.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631953.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1971344
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT88380.44832358025793567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT77290.3920675437670949No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT71700.3637112548596288No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA33140.1681086608932789No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA30030.15233262180522528No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA27850.14127417639945133No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA27430.13914365022035727No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA26770.13579568051035235No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA25170.12767939030427972No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC24060.12204871397381685No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG23470.11905583196032757No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC21180.10743939160288615No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT20260.10277252473439441No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA19930.10109853987939194No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG19730.10008400360363284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35100.028.0925921
TTCGCTA2000.021.27499826
GCAGTCG6200.018.7983869
TCGCTAC2400.018.527
TATTAGA1601.8189894E-1218.52
AAACACG1900.018.515
ATACACA5600.018.537
TACCTTA2300.018.49999831
ATTTCGC2650.018.15094424
CTTATAC23650.018.14799137
CTCTATG10150.017.862071
TAAACCG1054.801568E-717.6190475
GTATCAA56400.017.351952
CGTGCCA12700.017.33464610
GCGGTAA12100.017.27686123
TTCTGCG11150.017.25560618
CCGCTTA1950.017.07692325
TAGACTC650.001580476417.0769215
AGTCGGT6850.017.01459911
AACTCCG12750.016.976475
Produced by FastQC (version 0.11.5)