FastQCFastQC Report
Fri 10 Feb 2017
ERR1631952.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631952.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62108
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3080.4959103497134024No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2600.4186256198879372No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2340.37676305789914344No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2280.36710246667096025No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2080.3349004959103497No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2020.32523990468216657No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1980.31879951053004446No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1860.2994783280736781No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1850.29786822953564757No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1650.265666258775037No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1590.25600566754685383No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1570.2527854704707928No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1470.23668448509048756No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1440.23185418947639594No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1420.2286339924003349No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1370.2205834997101823No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1340.21575320409609067No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1290.20770271140593805No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1290.20770271140593805No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1260.20287241579184648No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1210.19482192310169383No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1210.19482192310169383No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1190.19160172602563277No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1180.18999162748760223No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1150.18516133187351067No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1150.18516133187351067No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1140.18355123333548012No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1140.18355123333548012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1090.1755007406453275No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1090.1755007406453275No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1070.17228054356926645No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1050.16906034649320537No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1050.16906034649320537No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1040.16745024795517485No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1000.16100985380305274No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1000.16100985380305274No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG990.15939975526502223No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT980.1577896567269917No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA970.15617955818896118No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC970.15617955818896118No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC970.15617955818896118No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC970.15617955818896118No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC950.1529593611129001No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT950.1529593611129001No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG950.1529593611129001No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG940.15134926257486958No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG930.14973916403683904No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA920.14812906549880853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT900.14490886842274747No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC900.14490886842274747No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC900.14490886842274747No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC890.14329876988471696No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA880.14168867134668642No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC860.13846847427062536No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT840.1352482771945643No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG840.1352482771945643No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC840.1352482771945643No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC830.13363817865653377No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG800.1288078830424422No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA800.1288078830424422No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG790.1271977845044117No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT780.12558768596638115No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA780.12558768596638115No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA780.12558768596638115No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA780.12558768596638115No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC760.12236748889032009No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG740.11914729181425902No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT720.11592709473819797No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC720.11592709473819797No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT710.11431699620016746No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG710.11431699620016746No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA710.11431699620016746No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC710.11431699620016746No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT700.11270689766213693No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG690.11109679912410639No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG690.11109679912410639No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA680.10948670058607587No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG670.10787660204804533No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC670.10787660204804533No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT660.10626650351001482No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC650.1046564049719843No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG650.1046564049719843No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA650.1046564049719843No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT640.10304630643395375No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC630.10143620789592324No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT630.10143620789592324No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG630.10143620789592324No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG630.10143620789592324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAG250.00545689329.59999836
GGTATCA1200.027.7500021
GTCAATG451.303629E-424.66666631
TTCTTCT451.303629E-424.66666615
CTCTATG400.001910801923.1251
AACGTCA502.662434E-422.19999928
GGGTAAC502.662434E-422.19999924
TTCTGCG502.662434E-422.19999918
TCTGCGG502.662434E-422.19999919
CTGCGGG502.662434E-422.19999920
TCTTCTG502.662434E-422.19999916
GTAACGT502.662434E-422.19999926
TGCGGGT502.662434E-422.19999921
CTTCTGC502.662434E-422.19999917
GGTAACG502.662434E-422.19999925
TCTATGG450.003785807620.5555552
TAACGTC555.0682016E-420.18181827
GCAGCCG1101.6625563E-920.18181817
GCTTGCT555.0682016E-420.18181814
ACGCAGG656.768753E-519.92307722
Produced by FastQC (version 0.11.5)