FastQCFastQC Report
Fri 10 Feb 2017
ERR1631951.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631951.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78430
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1950.24862935101364275No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1940.24735432870075227No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1840.23460410557184752No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1670.21292872625270942No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1620.20655361468825706No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1590.2027285477495856No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1440.18360321305622848No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1370.17467805686599513No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT1340.17085298992732373No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC1330.16957796761443325No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1300.16575290067576182No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1290.16447787836287137No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1240.15810276679841898No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1230.1568277444855285No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1000.12750223128904756No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA970.12367716435037614No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA940.1198520974117047No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA930.11857707509881424No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC910.11602703047303327No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT890.11347698584725233No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA880.11220196353436186No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA880.11220196353436186No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT820.104551829657019No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT810.10327680734412853No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC800.10200178503123804No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAAGC251.2231636E-437.030
AGGCCAT251.2231636E-437.025
TGTACTT200.001831495637.05
AAGCAGA303.5692396E-430.83333233
TCAAGCA303.5692396E-430.83333231
CTGTACT250.00546554629.64
CCGTCCA250.00546554629.69
GTTACTG250.00546554629.615
CCTCACA250.00546554629.62
GTCCACG250.00546554629.611
GGTATCA1400.029.071431
CCAGGAC453.945992E-628.7777793
GTGTGGG405.8708934E-527.75000217
CATCAGA405.8708934E-527.75000216
AGAAGAG556.1375795E-726.9090920
GCAGATC358.795585E-426.42857235
CATCAAG358.795585E-426.42857229
TGTGGGG508.95085E-625.90000218
AGAGGCC508.95085E-625.90000223
GACAGGC451.3079135E-424.6666687
Produced by FastQC (version 0.11.5)