##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631951.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	78430
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52232564069871	34.0	31.0	34.0	31.0	34.0
2	32.68067066173658	34.0	31.0	34.0	31.0	34.0
3	32.77664159122784	34.0	31.0	34.0	31.0	34.0
4	36.22413617238302	37.0	37.0	37.0	35.0	37.0
5	36.1400102001785	37.0	35.0	37.0	35.0	37.0
6	36.19115134514854	37.0	36.0	37.0	35.0	37.0
7	36.178783628713504	37.0	36.0	37.0	35.0	37.0
8	36.15398444472778	37.0	36.0	37.0	35.0	37.0
9	37.99537166900421	39.0	38.0	39.0	35.0	39.0
10	37.92037485655999	39.0	38.0	39.0	35.0	39.0
11	38.00369756470738	39.0	38.0	39.0	35.0	39.0
12	37.92223638913681	39.0	38.0	39.0	35.0	39.0
13	37.97293127629734	39.0	38.0	39.0	35.0	39.0
14	39.30452632921076	40.0	39.0	41.0	36.0	41.0
15	39.30363381359174	40.0	39.0	41.0	36.0	41.0
16	39.26347061073569	40.0	39.0	41.0	36.0	41.0
17	39.23126354711207	40.0	39.0	41.0	36.0	41.0
18	39.23211781206171	40.0	39.0	41.0	36.0	41.0
19	39.25087339028433	40.0	39.0	41.0	36.0	41.0
20	39.23526711717455	40.0	39.0	41.0	36.0	41.0
21	39.182621445875306	40.0	39.0	41.0	36.0	41.0
22	39.156037230651535	40.0	39.0	41.0	36.0	41.0
23	39.074231799056484	40.0	39.0	41.0	35.0	41.0
24	39.07590207828637	40.0	39.0	41.0	35.0	41.0
25	39.03335458370521	40.0	39.0	41.0	35.0	41.0
26	38.94196098431723	40.0	38.0	41.0	35.0	41.0
27	38.81350248629351	40.0	38.0	41.0	35.0	41.0
28	38.811028943006505	40.0	38.0	41.0	35.0	41.0
29	38.76964171873008	40.0	38.0	41.0	35.0	41.0
30	38.73295932678822	40.0	38.0	41.0	35.0	41.0
31	38.66364911385949	40.0	38.0	41.0	35.0	41.0
32	38.59446640316205	40.0	38.0	41.0	35.0	41.0
33	38.513642738747926	40.0	38.0	41.0	34.0	41.0
34	38.474907560882315	40.0	38.0	41.0	34.0	41.0
35	38.39445365293893	40.0	38.0	41.0	34.0	41.0
36	38.35311742955502	40.0	38.0	41.0	34.0	41.0
37	38.292834374601554	40.0	37.0	41.0	34.0	41.0
38	38.23433635088614	40.0	37.0	41.0	34.0	41.0
39	38.15195715925029	40.0	37.0	41.0	34.0	41.0
40	37.86767818436822	40.0	37.0	41.0	33.0	41.0
41	37.9158357771261	40.0	37.0	41.0	33.0	41.0
42	37.887606783118706	40.0	36.0	41.0	33.0	41.0
43	37.372000510008924	39.0	35.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	3.0
21	10.0
22	18.0
23	37.0
24	50.0
25	84.0
26	135.0
27	193.0
28	318.0
29	452.0
30	630.0
31	831.0
32	1115.0
33	1588.0
34	2370.0
35	3573.0
36	5234.0
37	8846.0
38	18942.0
39	33999.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.17353053678439	15.555272217263802	12.04131072293765	29.229886523014155
2	21.731480300905265	18.610225678949384	30.897615708274895	28.760678311870457
3	21.132219813846742	19.16358536274385	29.189085809001657	30.515109014407756
4	15.747800586510262	15.239066683666962	33.38773428534999	35.62539844447278
5	17.69348463598113	31.825831952059165	32.40341705979854	18.07726635216116
6	34.98406222108887	33.02817799311488	15.263292107611884	16.724467678184368
7	30.98941731480301	27.118449572867526	19.260487058523523	22.631646053805945
8	28.323345658549027	29.60091801606528	19.897998214968762	22.177738110416932
9	29.277062348591098	12.054060946066556	16.997322453142928	41.671554252199414
10	19.776871095244168	22.277189850822392	28.513323983169702	29.43261507076374
11	40.59416039780696	18.534999362488843	17.915338518424072	22.955501721280122
12	24.50465383144205	23.030728037740662	25.089889073058778	27.37472905775851
13	35.653448935356366	16.438862680096904	20.07650133877343	27.831187045773305
14	24.56202983552212	19.583067703684815	22.3026902970802	33.552212163712866
15	30.418207318628077	23.312507968889456	19.260487058523523	27.008797653958943
16	26.23995919928599	23.075353818691827	20.96264184623231	29.722045135789877
17	28.374346551064644	23.0524034170598	20.096901695779675	28.47634833609588
18	27.54813209231162	20.00255004462578	22.745123039653194	29.70419482340941
19	29.17378554124697	21.29287262527094	22.04258574525054	27.490756088231542
20	30.192528369246464	19.835522121637126	21.74550554634706	28.22644396276935
21	29.373964044370776	20.497258702027285	21.02001785031238	29.108759403289557
22	30.25245441795231	21.583577712609973	19.54354201198521	28.620425857452503
23	29.479790896340685	20.706362361341323	20.75353818691827	29.060308555399722
24	29.533341833482083	20.652811424199925	21.37319903098304	28.44064771133495
25	28.698202218538825	20.740787963789366	21.736580390156828	28.824429427514982
26	29.464490628586	21.360448807854137	21.29032258064516	27.884737982914704
27	28.890730587785285	20.47940838964682	22.84202473543287	27.787836287135026
28	27.13119979599643	21.436950146627566	21.31454800459008	30.11730205278592
29	28.268519699094735	22.41234221598878	21.721280122402142	27.597857962514343
30	27.2829274512304	20.683411959709293	23.160780313655486	28.87288027540482
31	28.865230141527476	21.044243274257298	21.997959964299373	28.09256661991585
32	26.11373199030983	21.06591865357644	21.800331505801353	31.02001785031238
33	26.933571337498407	21.333673339283436	23.209231161545326	28.52352416167283
34	27.226826469463216	20.97284202473543	22.909600918016064	28.890730587785285
35	27.018997832462066	22.669896723192657	23.438735177865613	26.872370266479663
36	27.545582047685834	21.457350503633812	23.281907433380084	27.715160015300267
37	26.886395511921457	21.545327043223256	23.64783883717965	27.920438607675635
38	27.07892388116792	20.7229376514089	24.568404947086574	27.629733520336607
39	25.699349738620427	20.26010455182966	25.253091929108763	28.787453780441158
40	25.907178375621577	20.739512941476477	26.071656253984443	27.281652428917507
41	24.12724722682647	19.769220961366823	27.337753410684684	28.76577840112202
42	22.92235114114497	20.93841642228739	28.111691954609206	28.027540481958436
43	21.98010965191891	20.597985464745634	28.55029963024353	28.871605253091932
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	1.5
4	1.0
5	1.0
6	3.5
7	6.0
8	4.0
9	2.0
10	5.5
11	9.0
12	9.0
13	21.5
14	34.0
15	67.0
16	100.0
17	82.0
18	64.0
19	64.0
20	88.0
21	112.0
22	89.0
23	66.0
24	87.0
25	108.0
26	108.0
27	113.5
28	119.0
29	160.0
30	201.0
31	264.0
32	327.0
33	327.0
34	496.5
35	666.0
36	746.0
37	826.0
38	1112.5
39	1399.0
40	1399.0
41	1690.5
42	1982.0
43	2484.5
44	2987.0
45	3968.5
46	4950.0
47	4950.0
48	5595.0
49	6240.0
50	7244.0
51	8248.0
52	8552.0
53	8856.0
54	8856.0
55	8295.5
56	7735.0
57	7560.0
58	7385.0
59	6913.5
60	6442.0
61	6442.0
62	6068.5
63	5695.0
64	4562.5
65	3430.0
66	3043.5
67	2657.0
68	2657.0
69	2297.0
70	1937.0
71	1830.0
72	1723.0
73	1697.5
74	1672.0
75	1672.0
76	1375.0
77	1078.0
78	796.0
79	514.0
80	404.5
81	295.0
82	295.0
83	281.0
84	267.0
85	214.5
86	162.0
87	121.0
88	80.0
89	80.0
90	58.0
91	36.0
92	23.5
93	11.0
94	8.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	78430.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.61379574142547
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.37004725554344	43.338008415147264
2	7.267660244759481	7.647583832717072
3	2.9734641948382405	4.69335713374984
4	1.6260753665333818	3.4221598877980366
5	1.1074760693081305	2.9134259849547366
6	0.7778989458378771	2.455692974627056
7	0.6494608021325579	2.3919418589825323
8	0.40470132073185505	1.7034298100216754
9	0.3853144311159578	1.8245569297462707
>10	2.263419362656004	21.91125844702282
>50	0.14055494971525506	5.001912533469335
>100	0.033927056827820185	2.696672191763356
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	195	0.24862935101364275	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	194	0.24735432870075227	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	184	0.23460410557184752	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	167	0.21292872625270942	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	162	0.20655361468825706	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	159	0.2027285477495856	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	144	0.18360321305622848	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	137	0.17467805686599513	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	134	0.17085298992732373	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	133	0.16957796761443325	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	130	0.16575290067576182	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	129	0.16447787836287137	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	124	0.15810276679841898	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	123	0.1568277444855285	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	100	0.12750223128904756	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	97	0.12367716435037614	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	94	0.1198520974117047	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	93	0.11857707509881424	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	91	0.11602703047303327	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	89	0.11347698584725233	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	88	0.11220196353436186	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	88	0.11220196353436186	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	82	0.104551829657019	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	81	0.10327680734412853	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	80	0.10200178503123804	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0012750223128904757	0.0	0.0	0.0	0.0
20	0.0012750223128904757	0.0	0.0	0.0	0.0
21	0.0012750223128904757	0.0	0.0	0.0	0.0
22	0.0012750223128904757	0.0	0.0	0.0012750223128904757	0.0
23	0.0012750223128904757	0.0	0.0	0.0012750223128904757	0.0
24	0.0012750223128904757	0.0	0.0	0.003825066938671427	0.0
25	0.0012750223128904757	0.0	0.0	0.007650133877342854	0.0
26	0.0012750223128904757	0.0	0.0	0.00892515619023333	0.0
27	0.0012750223128904757	0.0	0.0	0.030600535509371415	0.0
28	0.0012750223128904757	0.0	0.0	0.11730205278592376	0.0
29	0.0012750223128904757	0.0	0.0	0.25755450720387607	0.0
30	0.0012750223128904757	0.0	0.0	0.4335075863827617	0.0
31	0.0012750223128904757	0.0	0.0	0.8784903735815377	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCAAGC	25	1.2231636E-4	37.0	30
AGGCCAT	25	1.2231636E-4	37.0	25
TGTACTT	20	0.0018314956	37.0	5
AAGCAGA	30	3.5692396E-4	30.833332	33
TCAAGCA	30	3.5692396E-4	30.833332	31
CTGTACT	25	0.005465546	29.6	4
CCGTCCA	25	0.005465546	29.6	9
GTTACTG	25	0.005465546	29.6	15
CCTCACA	25	0.005465546	29.6	2
GTCCACG	25	0.005465546	29.6	11
GGTATCA	140	0.0	29.07143	1
CCAGGAC	45	3.945992E-6	28.777779	3
GTGTGGG	40	5.8708934E-5	27.750002	17
CATCAGA	40	5.8708934E-5	27.750002	16
AGAAGAG	55	6.1375795E-7	26.90909	20
GCAGATC	35	8.795585E-4	26.428572	35
CATCAAG	35	8.795585E-4	26.428572	29
TGTGGGG	50	8.95085E-6	25.900002	18
AGAGGCC	50	8.95085E-6	25.900002	23
GACAGGC	45	1.3079135E-4	24.666668	7
>>END_MODULE