FastQCFastQC Report
Fri 10 Feb 2017
ERR1631950.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631950.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608126
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13390.22018463278991526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12590.20702946428865068No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10760.1769370163420081No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA10520.1729904657916287No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9960.16378184784074354No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9590.1576975824089087No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9400.15457322988985836No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9380.15424435067732672No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9090.14947560209561836No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8890.14618680997030223No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8580.1410891821760622No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC8350.13730707123194866No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8330.13697819201941702No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7620.12530297997454476No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7340.12069867099910217No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6400.10524134801011634No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6380.10491246879758472No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6280.10326807273492664No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6250.10277475391612922No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAAGC200.001841254537.02
TAGACCC250.00549433429.5999984
CTATAAG250.00549433429.5999981
TATACTG250.00549433429.5999985
GGTATCA10400.024.9038471
CTACCGT551.9007493E-523.5454548
TATTAGA802.7204806E-823.1252
CCGAAAT400.001930147123.12519
CCGCTTA652.6793969E-622.7692325
GGATATA502.7000753E-422.1999992
GTATTAG1350.021.9259261
ACCGTTT603.7225735E-521.58333210
TATACAC2300.020.91304237
TATAATC450.003823767420.5555553
GATATAC555.1392673E-420.1818183
CGTGTCT555.1392673E-420.18181810
TGCGGGT2300.020.10869421
CTCTATG2350.018.8936161
TCTATGG2500.018.5000022
TAATACT902.1488268E-618.54
Produced by FastQC (version 0.11.5)