##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631950.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	608126
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.608650181048006	34.0	31.0	34.0	31.0	34.0
2	32.772311987976174	34.0	31.0	34.0	31.0	34.0
3	32.86340166347106	34.0	31.0	34.0	31.0	34.0
4	36.28825111901152	37.0	37.0	37.0	35.0	37.0
5	36.20766420116884	37.0	35.0	37.0	35.0	37.0
6	36.26161848038071	37.0	37.0	37.0	35.0	37.0
7	36.250962793894686	37.0	37.0	37.0	35.0	37.0
8	36.228709181978736	37.0	37.0	37.0	35.0	37.0
9	38.06190328977876	39.0	38.0	39.0	35.0	39.0
10	38.002435350568796	39.0	38.0	39.0	35.0	39.0
11	38.073058872667836	39.0	38.0	39.0	37.0	39.0
12	38.0048263024439	39.0	38.0	39.0	35.0	39.0
13	38.058085002121274	39.0	38.0	39.0	35.0	39.0
14	39.440198248389315	40.0	39.0	41.0	37.0	41.0
15	39.44519721241979	41.0	39.0	41.0	37.0	41.0
16	39.39237592209509	40.0	39.0	41.0	37.0	41.0
17	39.36303825194121	40.0	39.0	41.0	36.0	41.0
18	39.38671591084742	40.0	39.0	41.0	37.0	41.0
19	39.423815130417054	41.0	39.0	41.0	37.0	41.0
20	39.4113111427566	41.0	39.0	41.0	37.0	41.0
21	39.37981438057245	40.0	39.0	41.0	37.0	41.0
22	39.34760230610104	40.0	39.0	41.0	36.0	41.0
23	39.28727928093849	40.0	39.0	41.0	36.0	41.0
24	39.300825815702666	40.0	39.0	41.0	36.0	41.0
25	39.246642965438085	40.0	39.0	41.0	36.0	41.0
26	39.182583872421176	40.0	39.0	41.0	36.0	41.0
27	39.08266543446588	40.0	39.0	41.0	35.0	41.0
28	39.074236260248696	40.0	39.0	41.0	35.0	41.0
29	39.052581208499554	40.0	39.0	41.0	35.0	41.0
30	38.99865159522862	40.0	39.0	41.0	35.0	41.0
31	38.913052558186955	40.0	38.0	41.0	35.0	41.0
32	38.863276689370295	40.0	38.0	41.0	35.0	41.0
33	38.80288459957311	40.0	38.0	41.0	35.0	41.0
34	38.77971834784239	40.0	38.0	41.0	35.0	41.0
35	38.71113716565317	40.0	38.0	41.0	35.0	41.0
36	38.65754794236721	40.0	38.0	41.0	35.0	41.0
37	38.61411450916422	40.0	38.0	41.0	35.0	41.0
38	38.57171868987677	40.0	38.0	41.0	35.0	41.0
39	38.50890769347142	40.0	38.0	41.0	35.0	41.0
40	38.211827811999484	40.0	38.0	41.0	34.0	41.0
41	38.27054261781276	40.0	38.0	41.0	34.0	41.0
42	38.243977399420515	40.0	37.0	41.0	34.0	41.0
43	37.77039133337499	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	4.0
19	14.0
20	22.0
21	47.0
22	113.0
23	158.0
24	304.0
25	538.0
26	780.0
27	1320.0
28	2093.0
29	2884.0
30	4275.0
31	5638.0
32	7573.0
33	10398.0
34	15180.0
35	22598.0
36	34680.0
37	63130.0
38	143714.0
39	292660.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.67536661810217	16.914422340107148	13.189700818580361	27.22051022321032
2	20.069360625922915	19.676843285766438	33.912215560591065	26.341580527719582
3	20.79667700443658	20.665947517455262	29.831975610317595	28.705399867790554
4	15.336459878380468	16.4010418893453	34.4780522457517	33.784445986522535
5	16.515820734518833	33.18868129302151	33.8538395003667	16.441658472092954
6	33.0964306739064	34.376099689866905	15.896212298109274	16.63125733811743
7	29.582356288006107	28.471731187286846	20.682062598869315	21.26384992583774
8	26.685588184027655	31.7753228771669	20.511703166777938	21.027385772027507
9	27.973150301088918	13.574653936848613	18.533330263794014	39.91886549826845
10	19.049999506681182	24.091060076365753	30.478223262942215	26.380717154010846
11	37.64647457928127	20.525187214491734	19.692793927574222	22.13554427865278
12	22.447979530557813	24.544420070840584	27.24501172454393	25.762588674057678
13	33.51065404208996	18.56062723843414	22.077661537247213	25.851057182228683
14	23.692951789596233	20.794210410342593	24.14729842170866	31.365539378352516
15	28.684680477401063	24.914902503757443	21.163870645228126	25.23654637361336
16	24.898787422343396	24.848140023613528	23.39432946461753	26.858743089425545
17	25.50902280119581	24.596711865633107	23.110177824990217	26.78408750818087
18	25.486165695924857	22.370857355219147	24.941212840759974	27.20176410809602
19	27.116091073231534	23.189602154816598	24.682384900497595	25.011921871454273
20	28.40842193887451	21.81982023462243	24.339363881827122	25.43239394467594
21	26.33319410780003	23.359961586907975	23.286621522513425	27.020222782778568
22	27.064128157651542	23.939611198994946	22.875193627636378	26.121067015717138
23	26.63625630214791	23.231863133626913	24.00637367913886	26.125506885086313
24	26.729822438113153	22.87585138606144	23.981707738198992	26.412618437626413
25	26.571795976491714	23.029931297132503	24.53899356383381	25.85927916254197
26	26.574920329010766	23.58294169300441	23.878768544676596	25.96336943330823
27	26.362464357715343	22.76403245380069	24.821007488579667	26.0524956999043
28	25.327481475878354	23.675192312119528	23.771882800603823	27.2254434113983
29	26.300799505365664	24.01278682378323	23.809375030832424	25.87703864001868
30	25.782156987203308	23.34647753919418	24.9227956048582	25.948569868744308
31	26.437613257778814	23.184997845841156	24.325715394507057	26.05167350187297
32	24.456116002275845	23.37558334950323	24.353670127572247	27.81463052064868
33	24.71773941584474	22.981750492496623	25.980800031572404	26.319710060086233
34	25.174059323232356	22.60715706942311	25.47547712151758	26.743306485826952
35	24.93233310202162	24.000289413707026	25.584007261653014	25.48337022261834
36	25.17636147772008	22.983066009346746	25.801067541923878	26.039504971009297
37	25.436176055620052	22.57936677596419	25.607851004561553	26.3766061638542
38	24.884316736992005	21.854188112331986	26.567684986335067	26.693810164340942
39	24.389024642919395	21.08888618477092	27.73783722452255	26.784251947787137
40	24.006702558351396	22.217435202573153	28.150745075855994	25.625117163219464
41	22.557002989512043	21.36136261235336	29.266961123188285	26.81467327494631
42	21.435689314385506	21.750097841565726	29.715552369081404	27.098660474967357
43	20.90109615441537	22.361155418449467	29.50030092447947	27.237447502655698
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11.0
1	13.0
2	15.0
3	35.5
4	56.0
5	56.0
6	90.0
7	124.0
8	118.5
9	113.0
10	196.0
11	279.0
12	279.0
13	584.5
14	890.0
15	1665.0
16	2440.0
17	2341.0
18	2242.0
19	2242.0
20	2571.5
21	2901.0
22	2487.5
23	2074.0
24	2279.5
25	2485.0
26	2485.0
27	2827.5
28	3170.0
29	4261.5
30	5353.0
31	6411.5
32	7470.0
33	7470.0
34	9062.0
35	10654.0
36	11849.0
37	13044.0
38	16042.5
39	19041.0
40	19041.0
41	21892.5
42	24744.0
43	27930.0
44	31116.0
45	39095.5
46	47075.0
47	47075.0
48	52033.0
49	56991.0
50	63294.5
51	69598.0
52	69861.0
53	70124.0
54	70124.0
55	63503.0
56	56882.0
57	52889.0
58	48896.0
59	43497.5
60	38099.0
61	38099.0
62	34257.5
63	30416.0
64	24972.5
65	19529.0
66	16714.5
67	13900.0
68	13900.0
69	11947.5
70	9995.0
71	8499.5
72	7004.0
73	5684.0
74	4364.0
75	4364.0
76	3506.5
77	2649.0
78	2233.5
79	1818.0
80	1443.0
81	1068.0
82	1068.0
83	876.0
84	684.0
85	549.0
86	414.0
87	310.5
88	207.0
89	207.0
90	175.0
91	143.0
92	91.5
93	40.0
94	23.5
95	7.0
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	608126.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.46112767655543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.95627487252568	40.79581736326035
2	6.269656596061744	5.951299443876888
3	2.2177289583536917	3.1576775173325653
4	1.145873046416584	2.1753770782840407
5	0.7438795224630768	1.7652680495797588
6	0.5207355507050287	1.4828817874639666
7	0.3865718848571026	1.2842996308358743
8	0.2816849713945333	1.0695269113538248
9	0.2402801056474281	1.0263568295041932
>10	1.8592619413033056	18.073082178778215
>50	0.22871134971262888	7.567037354379033
>100	0.13959398933385525	12.04794606568148
>500	0.008354752478834231	2.8226040589967556
>1k	0.0013924587464723716	0.7808257306730664
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1339	0.22018463278991526	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1259	0.20702946428865068	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1076	0.1769370163420081	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1052	0.1729904657916287	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	996	0.16378184784074354	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	959	0.1576975824089087	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	940	0.15457322988985836	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	938	0.15424435067732672	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	909	0.14947560209561836	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	889	0.14618680997030223	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	858	0.1410891821760622	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	835	0.13730707123194866	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	833	0.13697819201941702	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	762	0.12530297997454476	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	734	0.12069867099910217	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	640	0.10524134801011634	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	638	0.10491246879758472	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	628	0.10326807273492664	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	625	0.10277475391612922	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.6443960626580677E-4	0.0
5	0.0	0.0	0.0	1.6443960626580677E-4	0.0
6	0.0	0.0	0.0	1.6443960626580677E-4	0.0
7	0.0	0.0	0.0	1.6443960626580677E-4	0.0
8	0.0	0.0	0.0	1.6443960626580677E-4	0.0
9	0.0	0.0	0.0	1.6443960626580677E-4	0.0
10	1.6443960626580677E-4	0.0	0.0	3.2887921253161353E-4	0.0
11	1.6443960626580677E-4	0.0	0.0	3.2887921253161353E-4	0.0
12	1.6443960626580677E-4	0.0	0.0	3.2887921253161353E-4	0.0
13	1.6443960626580677E-4	0.0	0.0	3.2887921253161353E-4	0.0
14	1.6443960626580677E-4	0.0	0.0	8.221980313290338E-4	0.0
15	1.6443960626580677E-4	0.0	0.0	0.0011510772438606473	0.0
16	1.6443960626580677E-4	0.0	0.0	0.001973275275189681	0.0
17	1.6443960626580677E-4	0.0	0.0	0.0023021544877212946	0.0
18	1.6443960626580677E-4	0.0	0.0	0.002795473306518715	0.0
19	1.6443960626580677E-4	0.0	0.0	0.004110990156645169	0.0
20	1.6443960626580677E-4	0.0	0.0	0.004604308975442589	0.0
21	1.6443960626580677E-4	0.0	0.0	0.007564221888227111	0.0
22	1.6443960626580677E-4	0.0	0.0	0.009866376375948406	0.0
23	1.6443960626580677E-4	0.0	0.0	0.01167521204487228	0.0
24	1.6443960626580677E-4	0.0	0.0	0.013977366532593575	0.0
25	3.2887921253161353E-4	0.0	0.0	0.015621762595251642	0.0
26	3.2887921253161353E-4	0.0	0.0	0.01874611511430197	0.0
27	3.2887921253161353E-4	0.0	0.0	0.03946550550379362	0.0
28	3.2887921253161353E-4	0.0	0.0	0.13566267516929056	0.0
29	3.2887921253161353E-4	0.0	0.0	0.29056478427168053	0.0
30	3.2887921253161353E-4	0.0	0.0	0.49825200698539446	0.0
31	3.2887921253161353E-4	0.0	0.0	1.095167777730273	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAAGC	20	0.0018412545	37.0	2
TAGACCC	25	0.005494334	29.599998	4
CTATAAG	25	0.005494334	29.599998	1
TATACTG	25	0.005494334	29.599998	5
GGTATCA	1040	0.0	24.903847	1
CTACCGT	55	1.9007493E-5	23.545454	8
TATTAGA	80	2.7204806E-8	23.125	2
CCGAAAT	40	0.0019301471	23.125	19
CCGCTTA	65	2.6793969E-6	22.76923	25
GGATATA	50	2.7000753E-4	22.199999	2
GTATTAG	135	0.0	21.925926	1
ACCGTTT	60	3.7225735E-5	21.583332	10
TATACAC	230	0.0	20.913042	37
TATAATC	45	0.0038237674	20.555555	3
GATATAC	55	5.1392673E-4	20.181818	3
CGTGTCT	55	5.1392673E-4	20.181818	10
TGCGGGT	230	0.0	20.108694	21
CTCTATG	235	0.0	18.893616	1
TCTATGG	250	0.0	18.500002	2
TAATACT	90	2.1488268E-6	18.5	4
>>END_MODULE