FastQCFastQC Report
Fri 10 Feb 2017
ERR1631949.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631949.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917469
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16240.17700870547124753No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA16150.1760277458965916No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA14280.1556455858454073No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA13990.15248471610484932No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC12800.13951425061773204No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12290.13395547969468177No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA11460.12490885250618822No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG11180.12185697827392533No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT10330.11259236006884157No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10070.10975847685316888No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9610.10474468347159414No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT9540.10398171491352842No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9320.10158381373103614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8350.030.3532921
GTATAAT451.3229276E-424.6666681
ATACGGC1051.8189894E-1224.66666629
GCGGTAA6600.020.74242423
GTCTATA450.003825227720.5555572
AGTCGGT1950.019.92307711
TACGGCT1403.6379788E-1219.82142830
GTATCAA12650.019.7430842
TAATACT951.6751983E-719.4736844
GGTAATA7150.019.40559425
CGTGCCA7650.019.34640510
CGGTAAT7200.019.27083424
AACTCCG7350.019.1292515
CTAACTC7250.018.882763
CTTATAC8100.018.72839537
CGCGGTA7400.018.522
TCTCGCG902.1512515E-618.520
CTAGGAC609.234877E-418.53
TAACTCC7400.018.54
TACCGGT609.234877E-418.57
Produced by FastQC (version 0.11.5)