FastQCFastQC Report
Fri 10 Feb 2017
ERR1631948.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631948.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589466
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8280.140466116790451No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8040.136394635144351No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7710.13079634788096345No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7090.12027835362853838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7080.12010870855995089No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6990.11858190294266335No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6810.11552829170808833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6660.11298361567927583No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6490.1100996495132883No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6030.10229597635826324No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5960.10110846087815074No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGTC250.00549419829.63
ATCTTAT250.00549419829.61
GTACTAT250.00549419829.61
GGTATCA6700.028.1641771
CGTTATT556.253049E-726.9090910
AGGACCG1000.025.9000025
TGTTACG509.081279E-625.90000216
GACCGTT1051.8189894E-1224.6666667
CTTGGAC751.3707904E-824.6666663
GGACCGT1150.024.1304366
TAATACT802.7201168E-823.1250024
ATTAGAG855.1699317E-821.7647063
GTAAGAC450.003823629620.5555573
GTATTAG1650.020.1818181
GTTAGGA555.139009E-420.1818182
CTTAGGA2300.020.1086962
CTAAGTC656.8948415E-519.92307734
GTCTAGG656.8948415E-519.9230771
TTGTTAC656.8948415E-519.92307715
ATTAGCT1052.2539098E-819.380953