##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631948.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	589466
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61954887983361	34.0	31.0	34.0	31.0	34.0
2	32.78969270492276	34.0	31.0	34.0	31.0	34.0
3	32.884215544238344	34.0	31.0	34.0	31.0	34.0
4	36.30649774541704	37.0	37.0	37.0	35.0	37.0
5	36.2220094119084	37.0	35.0	37.0	35.0	37.0
6	36.27520840896676	37.0	37.0	37.0	35.0	37.0
7	36.259073466493405	37.0	37.0	37.0	35.0	37.0
8	36.23606789874225	37.0	37.0	37.0	35.0	37.0
9	38.08079685681617	39.0	38.0	39.0	37.0	39.0
10	38.01288454295922	39.0	38.0	39.0	35.0	39.0
11	38.08253232586782	39.0	38.0	39.0	37.0	39.0
12	38.021572066921586	39.0	38.0	39.0	35.0	39.0
13	38.06645166981641	39.0	38.0	39.0	37.0	39.0
14	39.47375251498814	40.0	39.0	41.0	37.0	41.0
15	39.46778100857386	41.0	39.0	41.0	37.0	41.0
16	39.42549018942568	40.0	39.0	41.0	37.0	41.0
17	39.389338825309686	40.0	39.0	41.0	37.0	41.0
18	39.40664940810836	40.0	39.0	41.0	37.0	41.0
19	39.44116878666454	41.0	39.0	41.0	37.0	41.0
20	39.43033864548591	41.0	39.0	41.0	37.0	41.0
21	39.39567167572006	40.0	39.0	41.0	37.0	41.0
22	39.38222900048518	40.0	39.0	41.0	37.0	41.0
23	39.3196418453312	40.0	39.0	41.0	36.0	41.0
24	39.330624667071554	40.0	39.0	41.0	36.0	41.0
25	39.27613297459056	40.0	39.0	41.0	36.0	41.0
26	39.22663563292878	40.0	39.0	41.0	36.0	41.0
27	39.1215439058402	40.0	39.0	41.0	36.0	41.0
28	39.117199295633675	40.0	39.0	41.0	36.0	41.0
29	39.097854668462645	40.0	39.0	41.0	35.0	41.0
30	39.06300108912134	40.0	39.0	41.0	35.0	41.0
31	38.98667607631314	40.0	39.0	41.0	35.0	41.0
32	38.93645604665918	40.0	38.0	41.0	35.0	41.0
33	38.891671445002764	40.0	38.0	41.0	35.0	41.0
34	38.8616781968765	40.0	38.0	41.0	35.0	41.0
35	38.798446390461876	40.0	38.0	41.0	35.0	41.0
36	38.75913623516878	40.0	38.0	41.0	35.0	41.0
37	38.715055660547	40.0	38.0	41.0	35.0	41.0
38	38.674917637319204	40.0	38.0	41.0	35.0	41.0
39	38.625318508616274	40.0	38.0	41.0	35.0	41.0
40	38.33456891491621	40.0	38.0	41.0	34.0	41.0
41	38.40154818089593	40.0	38.0	41.0	34.0	41.0
42	38.38812755951997	40.0	38.0	41.0	34.0	41.0
43	37.92693047605799	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	3.0
18	5.0
19	11.0
20	24.0
21	47.0
22	74.0
23	143.0
24	264.0
25	457.0
26	757.0
27	1136.0
28	1787.0
29	2511.0
30	3640.0
31	5120.0
32	7101.0
33	9694.0
34	14481.0
35	21456.0
36	33347.0
37	59642.0
38	138170.0
39	289595.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.75123586432466	17.056115195787374	12.74373755229309	27.448911387594876
2	19.790793701417893	19.88409848914102	33.98838949150587	26.336718317935215
3	20.5874469435048	20.600000678580273	29.79561162136578	29.016940756549147
4	15.304360217552837	15.866563974851816	34.30189357825557	34.52718222933978
5	16.30051606029864	33.743591657534104	33.840289346628985	16.115602935538266
6	33.805681752637135	34.473574387666126	15.628382298554962	16.09236156114178
7	30.044820227120816	28.707338506376956	20.39727482161821	20.850566444884013
8	26.753536251454708	32.34622522757886	20.186066711226772	20.71417180973966
9	27.73306687747894	13.694937451863213	18.194433606009508	40.377562064648345
10	18.636528654748535	24.36832658711444	30.836044827012927	26.159099931124103
11	37.78267109553393	20.456820240692423	19.94652787438122	21.813980789392435
12	22.51817746909915	24.343728052169254	27.376812233445186	25.76128224528641
13	33.029216273712144	18.50047330974136	22.36939874394791	26.10091167259859
14	23.726559292647924	20.22762975303071	23.879579144513848	32.16623180980752
15	28.29289560381769	25.14564029138237	21.144391703677563	25.417072401122372
16	25.437769099490048	24.760545985688744	22.855431865451102	26.946253049370107
17	25.891400012893023	24.49589967869224	22.803181184326153	26.80951912408858
18	25.31884790641021	22.964683289621455	24.738831416909544	26.977637387058795
19	27.279944899281723	23.10345295572603	24.47910481690208	25.13749732809017
20	28.131563143590977	22.16582466164291	24.215815670454273	25.486796524311835
21	26.22492221773605	23.606789874225147	23.138399839855055	27.029888068183745
22	26.83276049848507	23.981535830734938	22.62675031299515	26.558953357784844
23	26.547078202983716	23.513145796364846	23.587789626543344	26.351986374108087
24	27.034129194898433	23.086827739004452	23.448511025233007	26.4305320408641
25	26.93115463826582	23.024228708695667	24.085528257779075	25.95908839525944
26	26.85108216589252	23.543172973504834	23.55911960995206	26.04662525065059
27	26.491434620487013	22.86187837805743	24.356960367519076	26.28972663393648
28	25.476617820196584	23.76472943308011	23.540458652407434	27.218194094315873
29	26.39151367508898	24.135234262875212	23.449868185781707	26.0233838762541
30	25.748219575005177	23.746747055809834	24.52440005021494	25.980633318970053
31	26.731312747469744	23.298035849395895	23.983062636352223	25.98758876678214
32	24.504381932121618	23.445457413998433	24.22582472950094	27.824335924379014
33	25.115952404379556	22.953147424957503	25.259472132404582	26.671428038258355
34	25.28746356872152	22.801824023777453	25.083719841347936	26.826992566153095
35	25.275588413920396	23.94794610715461	25.12986330000373	25.646602178921263
36	25.255739940895662	23.057648787207405	25.604021266705796	26.082590005191136
37	25.83049743327011	22.36600584257616	25.250311298700858	26.55318542545287
38	24.857582964920795	22.079475321731874	26.491434620487013	26.57150709286032
39	24.44449722291023	21.339144242416015	27.274516257086923	26.94184227758683
40	24.0376883484374	21.957161227280285	28.073035594928292	25.932114829354024
41	22.981987086617377	21.410225526154182	28.963332914875494	26.64445447235294
42	22.110011434077624	21.594460070640206	29.350802251529352	26.94472624375282
43	21.31929576939128	22.02196564348071	29.448857101172926	27.209881485955083
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	10.5
2	13.0
3	33.5
4	54.0
5	54.0
6	60.0
7	66.0
8	67.5
9	69.0
10	112.5
11	156.0
12	156.0
13	327.5
14	499.0
15	1007.5
16	1516.0
17	1529.5
18	1543.0
19	1543.0
20	1726.5
21	1910.0
22	1857.5
23	1805.0
24	2080.5
25	2356.0
26	2356.0
27	2724.5
28	3093.0
29	4401.0
30	5709.0
31	6712.5
32	7716.0
33	7716.0
34	9417.5
35	11119.0
36	12489.0
37	13859.0
38	17361.5
39	20864.0
40	20864.0
41	23936.0
42	27008.0
43	29290.0
44	31572.0
45	37799.0
46	44026.0
47	44026.0
48	48382.5
49	52739.0
50	58088.0
51	63437.0
52	65081.0
53	66725.0
54	66725.0
55	60203.0
56	53681.0
57	49720.5
58	45760.0
59	41334.0
60	36908.0
61	36908.0
62	33377.5
63	29847.0
64	25100.0
65	20353.0
66	17394.0
67	14435.0
68	14435.0
69	12500.0
70	10565.0
71	9014.5
72	7464.0
73	6062.0
74	4660.0
75	4660.0
76	3846.5
77	3033.0
78	2496.0
79	1959.0
80	1561.0
81	1163.0
82	1163.0
83	978.0
84	793.0
85	660.5
86	528.0
87	403.0
88	278.0
89	278.0
90	204.5
91	131.0
92	84.5
93	38.0
94	22.0
95	6.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	589466.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.42444249110029
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.78305110982704	43.75986969889691
2	6.128126159527525	6.180146902186059
3	2.014372149449772	3.047207778168124
4	1.0789033028287152	2.1761239018777885
5	0.664291428047688	1.6748262455460763
6	0.45283749683962693	1.3700446990322146
7	0.34570850494379596	1.220251103835589
8	0.268792737352212	1.0842979141313631
9	0.2133642046973883	0.9682893962480525
>10	1.6923024145171712	17.37468257752376
>50	0.223610552937442	7.814850471612505
>100	0.1299038607741846	11.736178062759507
>500	0.004736078257392146	1.5932312481820583
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	828	0.140466116790451	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	804	0.136394635144351	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	771	0.13079634788096345	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	709	0.12027835362853838	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	708	0.12010870855995089	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	699	0.11858190294266335	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	681	0.11552829170808833	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	666	0.11298361567927583	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	649	0.1100996495132883	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	603	0.10229597635826324	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	596	0.10110846087815074	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	3.3929013717500244E-4	0.0
15	0.0	0.0	0.0	5.089352057625037E-4	0.0
16	0.0	0.0	0.0	8.482253429375062E-4	0.0
17	0.0	0.0	0.0	0.0013571605487000097	0.0
18	1.6964506858750122E-4	0.0	0.0	0.0020357408230500147	0.0
19	1.6964506858750122E-4	0.0	0.0	0.0025446760288125184	0.0
20	1.6964506858750122E-4	0.0	0.0	0.0033929013717500247	0.0
21	1.6964506858750122E-4	0.0	0.0	0.004580416851862533	0.0
22	1.6964506858750122E-4	0.0	0.0	0.0071250928806750515	0.0
23	1.6964506858750122E-4	0.0	0.0	0.008482253429375062	0.0
24	1.6964506858750122E-4	0.0	0.0	0.011366219595362582	0.0
25	1.6964506858750122E-4	0.0	0.0	0.013062670281237594	0.0
26	1.6964506858750122E-4	0.0	0.0	0.015607346310050113	0.0
27	1.6964506858750122E-4	0.0	0.0	0.03409865878608775	0.0
28	1.6964506858750122E-4	0.0	0.0	0.11993906349136337	0.0
29	1.6964506858750122E-4	0.0	0.0	0.2526015071267893	0.0
30	1.6964506858750122E-4	0.0	0.0	0.4171572236566655	0.0
31	1.6964506858750122E-4	0.0	0.0	0.9391550997004068	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAGTC	25	0.005494198	29.6	3
ATCTTAT	25	0.005494198	29.6	1
GTACTAT	25	0.005494198	29.6	1
GGTATCA	670	0.0	28.164177	1
CGTTATT	55	6.253049E-7	26.90909	10
AGGACCG	100	0.0	25.900002	5
TGTTACG	50	9.081279E-6	25.900002	16
GACCGTT	105	1.8189894E-12	24.666666	7
CTTGGAC	75	1.3707904E-8	24.666666	3
GGACCGT	115	0.0	24.130436	6
TAATACT	80	2.7201168E-8	23.125002	4
ATTAGAG	85	5.1699317E-8	21.764706	3
GTAAGAC	45	0.0038236296	20.555557	3
GTATTAG	165	0.0	20.181818	1
GTTAGGA	55	5.139009E-4	20.181818	2
CTTAGGA	230	0.0	20.108696	2
CTAAGTC	65	6.8948415E-5	19.923077	34
GTCTAGG	65	6.8948415E-5	19.923077	1
TTGTTAC	65	6.8948415E-5	19.923077	15
ATTAGCT	105	2.2539098E-8	19.38095	3
>>END_MODULE