FastQCFastQC Report
Fri 10 Feb 2017
ERR1631947.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631947.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552456
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA19220.34790100931114876No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA18190.3292569906019665No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA14640.2649984795169208No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA14580.26391242017463834No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12960.23458881793301184No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC12640.2287965014408387No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12380.22409024429094807No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12260.22191812560638313No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10670.19313755303589789No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT10640.19259452336475663No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10520.19042240468019173No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10310.1866211969822031No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT9630.1743125244363352No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9440.1708733365191074No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8840.16001274309628277No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8400.15204830791954474No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8290.1500571991253602No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7730.13992064526405723No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7690.1391966057025356No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7670.13883458592177478No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC7620.13792953646987272No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7370.13340428921036246No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7330.13268024964884081No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT7310.13231822986808No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA7240.1310511606354171No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7090.12833601227971095No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA6980.12634490348552646No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6920.12525884414324398No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT6860.12417278480096151No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC6730.1218196562260162No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC6720.12163864633563577No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT6600.11946652765107085No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6510.11783743863764716No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC6470.11711339907612552No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT6360.11512229028194101No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC6340.11476027050118019No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6310.11421724083003895No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC6230.11276916170699566No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6220.11258815181661526No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA6220.11258815181661526No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5920.10715785510520294No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG5840.10570977598215966No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG5780.1046237166398772No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC5760.10426169685911638No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA5750.10408068696873597No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5730.10371866718797515No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG5730.10371866718797515No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA5660.10245159795531228No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT5550.10046048916112778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8150.026.558281
TTAGCAT451.3219973E-424.6666664
TAGCATG451.3219973E-424.6666665
AAGGATA400.001929924923.12533
GTTAGGG502.6996416E-422.21
GCGCAAA603.7217782E-521.58333422
CTTATAC4550.020.73626537
TAGCATC450.003823331320.5555555
CAGTATT450.003823331320.55555514
TTAGGGT555.1384483E-420.1818182
CTCTAAT555.1384483E-420.1818181
ATCGCTG555.1384483E-420.18181836
CCGCTTA656.8938396E-519.92307725
CGGCTAC656.8938396E-519.92307725
ACATACT656.8938396E-519.9230774
TATACAC1052.2530003E-819.38095337
GCGGTAA7350.018.6258523
GGTAATA7550.018.62251525
GTATCAA11450.018.5807862
ACAGTTA609.22854E-418.521
Produced by FastQC (version 0.11.5)