##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631947.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	552456
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55735660396484	34.0	31.0	34.0	31.0	34.0
2	32.71680097600533	34.0	31.0	34.0	31.0	34.0
3	32.813429485787104	34.0	31.0	34.0	31.0	34.0
4	36.248789043833355	37.0	37.0	37.0	35.0	37.0
5	36.16632636807275	37.0	35.0	37.0	35.0	37.0
6	36.21663987720289	37.0	36.0	37.0	35.0	37.0
7	36.21689691124723	37.0	36.0	37.0	35.0	37.0
8	36.19605724256773	37.0	36.0	37.0	35.0	37.0
9	38.0313092807391	39.0	38.0	39.0	35.0	39.0
10	37.9607914476447	39.0	38.0	39.0	35.0	39.0
11	38.037715220759665	39.0	38.0	39.0	35.0	39.0
12	37.95944473406027	39.0	38.0	39.0	35.0	39.0
13	38.01935176738057	39.0	38.0	39.0	35.0	39.0
14	39.37259799875465	40.0	39.0	41.0	37.0	41.0
15	39.39276431064194	40.0	39.0	41.0	37.0	41.0
16	39.32664501788378	40.0	39.0	41.0	36.0	41.0
17	39.30211274744052	40.0	39.0	41.0	36.0	41.0
18	39.327705735841405	40.0	39.0	41.0	36.0	41.0
19	39.34756795111285	40.0	39.0	41.0	36.0	41.0
20	39.34551348885703	40.0	39.0	41.0	36.0	41.0
21	39.304536469872716	40.0	39.0	41.0	36.0	41.0
22	39.277947927074734	40.0	39.0	41.0	36.0	41.0
23	39.22202492144171	40.0	39.0	41.0	36.0	41.0
24	39.235783483209524	40.0	39.0	41.0	36.0	41.0
25	39.17504199429457	40.0	39.0	41.0	36.0	41.0
26	39.10930282230621	40.0	39.0	41.0	36.0	41.0
27	38.96371656747325	40.0	39.0	41.0	35.0	41.0
28	38.97017862055983	40.0	39.0	41.0	35.0	41.0
29	38.95378274468917	40.0	38.0	41.0	35.0	41.0
30	38.92590903166949	40.0	38.0	41.0	35.0	41.0
31	38.837721013076155	40.0	38.0	41.0	35.0	41.0
32	38.79206850862331	40.0	38.0	41.0	35.0	41.0
33	38.72537179431484	40.0	38.0	41.0	35.0	41.0
34	38.70439274801975	40.0	38.0	41.0	35.0	41.0
35	38.63942829836222	40.0	38.0	41.0	35.0	41.0
36	38.58494432135772	40.0	38.0	41.0	35.0	41.0
37	38.530572570485255	40.0	38.0	41.0	35.0	41.0
38	38.488087739114064	40.0	38.0	41.0	34.0	41.0
39	38.39316796269748	40.0	38.0	41.0	34.0	41.0
40	38.10056909509536	40.0	37.0	41.0	34.0	41.0
41	38.160894261262435	40.0	37.0	41.0	34.0	41.0
42	38.1451753623818	40.0	37.0	41.0	34.0	41.0
43	37.658799976830736	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	0.0
17	0.0
18	2.0
19	7.0
20	25.0
21	46.0
22	82.0
23	153.0
24	274.0
25	449.0
26	739.0
27	1176.0
28	1859.0
29	2734.0
30	3924.0
31	5311.0
32	7280.0
33	9914.0
34	14648.0
35	21885.0
36	33540.0
37	60011.0
38	138203.0
39	250192.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.96197344222888	16.26138552210493	12.642816803510144	28.133824232156044
2	21.243320735044964	19.74166268444908	31.998023371997046	27.01699320850891
3	21.584343368521655	19.585813168831546	29.615028165138945	29.214815297507858
4	15.286828272296798	16.236949186903573	35.631977931274164	32.84424460952546
5	16.706307832659977	32.89619444806464	33.7596116251792	16.63788609409618
6	32.329452481283575	34.677331769407886	16.27496126388346	16.71825448542508
7	29.23888961292845	27.894529157073144	20.352933084263725	22.51364814573468
8	26.686288138783905	30.141042906584413	21.17616606571383	21.996502888917853
9	28.139254528867458	12.689698365118671	17.943510433410083	41.22753667260379
10	19.502910639037317	22.784257931853396	29.58317042443199	28.1296610046773
11	39.25670098614189	19.449150701594334	19.114101394500196	22.180046917763587
12	22.23398786509695	23.81746238614478	27.1437001317752	26.804849616983073
13	35.40517253862751	17.667470350579954	20.696489856205744	26.230867254586794
14	23.659078732061918	20.666261204512214	23.56278147037954	32.11187859304632
15	30.097238513112355	24.024537700739966	20.04666434974007	25.831559436407602
16	24.79744993266432	24.27704649782064	22.994410414585055	27.931093154929986
17	26.753804827895795	23.916655806073244	21.673762254369578	27.655777111661383
18	26.357393167962694	21.005835758865864	23.73455985635055	28.902211216820888
19	27.78212201514691	22.52577580839017	23.642606832037302	26.049495344425623
20	30.159324905512836	20.44361903934431	22.82045990992948	26.576596145213376
21	27.237644264882633	22.099497516544304	22.096420348407836	28.566437870165224
22	28.379273643511883	22.920015349638703	21.23807144822393	27.462639558625483
23	28.118619401364093	21.83793822494461	22.51292410617316	27.53051826751814
24	27.965847053883024	21.257077486713875	23.003279899213695	27.773795560189406
25	28.119705460706374	22.053339994497296	23.044550154220428	26.782404390575902
26	28.256729947724345	22.741901617504382	22.278878317911293	26.722490116859987
27	27.055005285488797	21.632673009253224	24.609380656559075	26.702941048698904
28	26.235573511736682	23.20908814457622	21.960300910841767	28.595037432845334
29	27.300635706735015	23.675007602415395	22.243581389287108	26.780775301562475
30	26.82114050711731	21.950888396541988	24.04354373922991	27.184427357110792
31	28.06558350348263	22.41952300273687	22.821183949491	26.6937095442895
32	25.616157666854917	22.233263825535428	22.82426111762747	29.326317389982187
33	25.665030337257626	22.007182472450296	24.88198155147197	27.445805638820104
34	26.050762413658283	22.121580723170712	23.931679626974816	27.895977236196185
35	25.678244059255395	24.14309917893914	24.273969329684174	25.904687432121293
36	26.294039706329553	22.536455391922612	24.859355315174422	26.310149586573413
37	27.281810678135454	22.465861534674257	23.96009817976454	26.29222960742575
38	26.162445516023	21.937312654763456	25.014118771449674	26.88612305776388
39	25.480943278740753	21.019230490754016	25.9794445168484	27.520381713656832
40	25.432613638009183	22.05605514285301	26.63669142881967	25.87463979031814
41	23.46069189220499	21.067379121595202	27.723836830444416	27.748092155755387
42	22.05370201427806	21.596109011396383	28.884834267344367	27.465354706981188
43	21.41491811112559	22.4589831588398	28.132557162923383	27.993541567111222
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	4.0
2	4.0
3	12.5
4	21.0
5	21.0
6	29.0
7	37.0
8	34.5
9	32.0
10	58.0
11	84.0
12	84.0
13	183.5
14	283.0
15	537.5
16	792.0
17	778.5
18	765.0
19	765.0
20	873.5
21	982.0
22	829.5
23	677.0
24	815.5
25	954.0
26	954.0
27	1162.5
28	1371.0
29	1777.5
30	2184.0
31	2690.5
32	3197.0
33	3197.0
34	4249.5
35	5302.0
36	5962.0
37	6622.0
38	8996.5
39	11371.0
40	11371.0
41	14094.0
42	16817.0
43	20160.0
44	23503.0
45	33814.5
46	44126.0
47	44126.0
48	49530.5
49	54935.0
50	61837.5
51	68740.0
52	69158.0
53	69576.0
54	69576.0
55	62945.0
56	56314.0
57	53019.0
58	49724.0
59	45523.5
60	41323.0
61	41323.0
62	37256.0
63	33189.0
64	26480.0
65	19771.0
66	16906.0
67	14041.0
68	14041.0
69	11885.5
70	9730.0
71	8214.0
72	6698.0
73	5182.0
74	3666.0
75	3666.0
76	2948.5
77	2231.0
78	1826.0
79	1421.0
80	1112.0
81	803.0
82	803.0
83	674.0
84	545.0
85	436.5
86	328.0
87	246.5
88	165.0
89	165.0
90	131.0
91	97.0
92	60.5
93	24.0
94	14.5
95	5.0
96	5.0
97	3.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	552456.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.834109409591
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.9503873671746	33.44088915361579
2	7.2103273246844	5.74433935060884
3	2.424480640923764	2.8973108133597756
4	1.2975879755022581	2.067530455389065
5	0.8401112086774264	1.673254090134017
6	0.5650965686420044	1.3506071125362034
7	0.41712524994292993	1.1631068990610764
8	0.34415248750590255	1.0967206272714418
9	0.25799684175464654	0.9249366979605174
>10	2.181434482061757	17.654457183937108
>50	0.2821753214503502	7.820047617197666
>100	0.20397113997854358	16.040880417208673
>500	0.0196653789665412	5.229153585330915
>1k	0.005488012734848706	2.896765996388896
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1922	0.34790100931114876	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1819	0.3292569906019665	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1464	0.2649984795169208	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1458	0.26391242017463834	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1296	0.23458881793301184	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1264	0.2287965014408387	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1238	0.22409024429094807	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1226	0.22191812560638313	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1067	0.19313755303589789	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1064	0.19259452336475663	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1052	0.19042240468019173	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1031	0.1866211969822031	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	963	0.1743125244363352	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	944	0.1708733365191074	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	884	0.16001274309628277	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	840	0.15204830791954474	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	829	0.1500571991253602	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	773	0.13992064526405723	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	769	0.1391966057025356	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	767	0.13883458592177478	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	762	0.13792953646987272	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	737	0.13340428921036246	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	733	0.13268024964884081	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	731	0.13231822986808	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	724	0.1310511606354171	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	709	0.12833601227971095	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	698	0.12634490348552646	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	692	0.12525884414324398	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	686	0.12417278480096151	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	673	0.1218196562260162	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	672	0.12163864633563577	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	660	0.11946652765107085	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	651	0.11783743863764716	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	647	0.11711339907612552	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	636	0.11512229028194101	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	634	0.11476027050118019	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	631	0.11421724083003895	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	623	0.11276916170699566	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	622	0.11258815181661526	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	622	0.11258815181661526	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	592	0.10715785510520294	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	584	0.10570977598215966	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	578	0.1046237166398772	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	576	0.10426169685911638	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	575	0.10408068696873597	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	573	0.10371866718797515	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	573	0.10371866718797515	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	566	0.10245159795531228	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	555	0.10046048916112778	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.810098903804104E-4	0.0	0.0	0.0	0.0
7	1.810098903804104E-4	0.0	0.0	0.0	0.0
8	5.430296711412311E-4	0.0	0.0	0.0	0.0
9	5.430296711412311E-4	0.0	0.0	0.0	0.0
10	7.240395615216416E-4	0.0	0.0	0.0	0.0
11	7.240395615216416E-4	0.0	0.0	0.0	0.0
12	7.240395615216416E-4	0.0	0.0	0.0	0.0
13	7.240395615216416E-4	0.0	0.0	0.0	0.0
14	7.240395615216416E-4	0.0	0.0	0.0	0.0
15	9.050494519020519E-4	0.0	0.0	1.810098903804104E-4	0.0
16	0.0010860593422824623	0.0	0.0	7.240395615216416E-4	0.0
17	0.0010860593422824623	0.0	0.0	9.050494519020519E-4	0.0
18	0.0012670692326628727	0.0	0.0	9.050494519020519E-4	0.0
19	0.0012670692326628727	0.0	0.0	0.0010860593422824623	0.0
20	0.0012670692326628727	0.0	0.0	0.0018100989038041038	0.0
21	0.0012670692326628727	0.0	0.0	0.0028961582460865663	0.0
22	0.0012670692326628727	0.0	0.0	0.0039822175883690285	0.0
23	0.0012670692326628727	0.0	0.0	0.00470625714989067	0.0
24	0.0012670692326628727	0.0	0.0	0.005611306601792722	0.0
25	0.0012670692326628727	0.0	0.0	0.006335346163314363	0.0
26	0.0012670692326628727	0.0	0.0	0.008145445067118468	0.0
27	0.0014480791230432831	0.0	0.0	0.0244363352013554	0.0
28	0.0016290890134236934	0.0	0.0	0.08941888584792274	0.0
29	0.0016290890134236934	0.0	0.0	0.205989255252907	0.0
30	0.0016290890134236934	0.0	0.0	0.35477938514560436	0.0
31	0.0016290890134236934	0.0	0.0	0.7506480154075619	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	815	0.0	26.55828	1
TTAGCAT	45	1.3219973E-4	24.666666	4
TAGCATG	45	1.3219973E-4	24.666666	5
AAGGATA	40	0.0019299249	23.125	33
GTTAGGG	50	2.6996416E-4	22.2	1
GCGCAAA	60	3.7217782E-5	21.583334	22
CTTATAC	455	0.0	20.736265	37
TAGCATC	45	0.0038233313	20.555555	5
CAGTATT	45	0.0038233313	20.555555	14
TTAGGGT	55	5.1384483E-4	20.181818	2
CTCTAAT	55	5.1384483E-4	20.181818	1
ATCGCTG	55	5.1384483E-4	20.181818	36
CCGCTTA	65	6.8938396E-5	19.923077	25
CGGCTAC	65	6.8938396E-5	19.923077	25
ACATACT	65	6.8938396E-5	19.923077	4
TATACAC	105	2.2530003E-8	19.380953	37
GCGGTAA	735	0.0	18.62585	23
GGTAATA	755	0.0	18.622515	25
GTATCAA	1145	0.0	18.580786	2
ACAGTTA	60	9.22854E-4	18.5	21
>>END_MODULE