FastQCFastQC Report
Fri 10 Feb 2017
ERR1631946.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631946.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131404
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3710.28233539313871725No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3450.2625490852637667No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3420.26026604973973394No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3200.24352378923016044No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3000.22830355240327538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2930.22297646951386563No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2560.19481903138412832No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2560.19481903138412832No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2550.1940580195427841No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2520.19177498401875134No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2420.18416486560530881No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2360.1795987945572433No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2360.1795987945572433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2250.17122766430245653No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2030.15448540379288303No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2020.15372439195153875No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1880.14307022617271925No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1760.13393808407658822No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1680.12784998934583422No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA1490.11339076436029344No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1460.11110772883626069No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG1420.10806368147088369No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1400.10654165778819519No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG1390.10578064594685094No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1370.10425862226416244No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA200.001836010337.01
TATACAC200.001836010337.037
GGTATCA2150.033.558141
TTTATAA303.5823064E-430.8333342
CTTATAC1350.026.03703737
AGTCGGT601.3224071E-624.66666811
TGACCGA1051.8189894E-1224.66666624
AAGGCTA1002.0008883E-1124.0500015
CCGATAG1103.6379788E-1223.54545427
GACCGAT1103.6379788E-1223.54545425
ACCGATA1103.6379788E-1223.54545426
GACTGAC1103.6379788E-1223.54545421
CGATAGT1103.6379788E-1223.54545428
GCAGTCG652.652945E-622.769239
ACTGACC1155.456968E-1222.5217422
TAGTGAA1155.456968E-1222.5217431
CCTGACT1155.456968E-1222.5217418
TGACTGA1155.456968E-1222.5217420
CTCTCTA502.6844948E-422.222
GCCTTAT603.6940597E-521.58333426
Produced by FastQC (version 0.11.5)