##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631946.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	131404
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57964749931509	34.0	31.0	34.0	31.0	34.0
2	32.742557304191656	34.0	31.0	34.0	31.0	34.0
3	32.821687315454625	34.0	31.0	34.0	31.0	34.0
4	36.27034945663755	37.0	37.0	37.0	35.0	37.0
5	36.18225472588353	37.0	35.0	37.0	35.0	37.0
6	36.23780859030167	37.0	37.0	37.0	35.0	37.0
7	36.228547076192505	37.0	37.0	37.0	35.0	37.0
8	36.20155398618002	37.0	36.0	37.0	35.0	37.0
9	38.057913001126295	39.0	38.0	39.0	35.0	39.0
10	37.96847127941311	39.0	38.0	39.0	35.0	39.0
11	38.04803506742565	39.0	38.0	39.0	35.0	39.0
12	37.982009680070625	39.0	38.0	39.0	35.0	39.0
13	38.033218166874676	39.0	38.0	39.0	35.0	39.0
14	39.37900672734468	40.0	39.0	41.0	37.0	41.0
15	39.38972177407081	40.0	39.0	41.0	37.0	41.0
16	39.32175580652035	40.0	39.0	41.0	36.0	41.0
17	39.3140848071596	40.0	39.0	41.0	36.0	41.0
18	39.36164804724361	40.0	39.0	41.0	36.0	41.0
19	39.375102736598585	40.0	39.0	41.0	37.0	41.0
20	39.3729642933244	40.0	39.0	41.0	37.0	41.0
21	39.34195306078963	40.0	39.0	41.0	36.0	41.0
22	39.322570089190584	40.0	39.0	41.0	36.0	41.0
23	39.25700130894037	40.0	39.0	41.0	36.0	41.0
24	39.2631350643816	40.0	39.0	41.0	36.0	41.0
25	39.213380110194514	40.0	39.0	41.0	36.0	41.0
26	39.15844266536787	40.0	39.0	41.0	36.0	41.0
27	39.048240540622814	40.0	39.0	41.0	35.0	41.0
28	39.04628474019056	40.0	39.0	41.0	35.0	41.0
29	39.02294450701653	40.0	39.0	41.0	35.0	41.0
30	38.972626404066844	40.0	38.0	41.0	35.0	41.0
31	38.88685275942893	40.0	38.0	41.0	35.0	41.0
32	38.83789686767526	40.0	38.0	41.0	35.0	41.0
33	38.7781346077745	40.0	38.0	41.0	35.0	41.0
34	38.74921615780342	40.0	38.0	41.0	35.0	41.0
35	38.684492100697085	40.0	38.0	41.0	35.0	41.0
36	38.635011110772886	40.0	38.0	41.0	35.0	41.0
37	38.59525585218106	40.0	38.0	41.0	35.0	41.0
38	38.55950351587471	40.0	38.0	41.0	35.0	41.0
39	38.505007457916044	40.0	38.0	41.0	35.0	41.0
40	38.211698274025146	40.0	38.0	41.0	34.0	41.0
41	38.28406289001857	40.0	38.0	41.0	34.0	41.0
42	38.26547898085294	40.0	37.0	41.0	34.0	41.0
43	37.78264740799367	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	2.0
18	2.0
19	2.0
20	7.0
21	9.0
22	19.0
23	28.0
24	51.0
25	119.0
26	145.0
27	257.0
28	396.0
29	639.0
30	943.0
31	1210.0
32	1694.0
33	2343.0
34	3351.0
35	5072.0
36	7865.0
37	14102.0
38	31381.0
39	61765.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.13189857234178	15.96526741956105	13.61374082980731	28.289093178289853
2	19.682810264527717	19.54354509756172	33.60019481903138	27.17344981887918
3	19.525280813369456	20.056467078627744	30.836199811269065	29.582052296733735
4	14.54445831177133	16.274238227146814	34.95631792030684	34.224985540775016
5	15.620529055432103	33.67249094395909	34.67322151532678	16.033758485282032
6	32.75851572250465	34.9228333992877	16.035280508964718	16.283370369242945
7	29.996803750266356	28.815713372500074	20.489482816352623	20.698000060880947
8	27.837813156372714	31.21670573194119	20.626464947794588	20.319016163891508
9	28.66122796870719	12.894584639736994	17.856381845301513	40.5878055462543
10	18.427140726309702	24.356183982222763	29.862865666189762	27.35380962527777
11	38.609935770600586	19.929378101123255	18.716325226020516	22.74436090225564
12	22.693373108885577	25.114912788042982	26.455815652491555	25.735898450579892
13	34.91370125719156	17.993363976743478	21.40954613253782	25.683388633527137
14	23.83489087090195	20.385985205929806	24.180390246872243	31.598733676296003
15	29.22970381419135	24.787677696264954	20.713981309549176	25.268637179994517
16	24.541109859669415	25.092082432802655	22.837204346899636	27.52960336062829
17	26.99232900063925	24.331070591458403	21.817448479498342	26.859151928404007
18	26.755654317981186	21.58610087972969	23.647681957931265	28.010562844357857
19	28.04024230617028	22.634014185260725	23.986332227329456	25.33941128123954
20	30.263918906578187	20.92021551855347	23.28848436881678	25.527381206051565
21	27.06766917293233	22.51681836169371	22.408754680222824	28.006757785151137
22	28.47325804389516	22.919393625764815	21.75961157955618	26.84773675078384
23	27.39186021734498	22.726096618063377	22.89656327052449	26.98547989406715
24	27.018203403244957	21.721560987488967	24.186478341602996	27.073757267663083
25	26.90481263888466	22.401905573650726	23.97111199050257	26.722169796962042
26	26.85230282183191	23.325012937201304	23.23293050439865	26.589753736568138
27	26.073787708136738	21.982588049070042	25.076101184134426	26.867523058658794
28	25.399531216705736	23.11116860978357	23.41633435816261	28.072965815348088
29	26.68640224041886	23.66975130133025	23.373717695047336	26.270128763203555
30	26.033454080545493	22.602051687924263	24.188000365285685	27.17649386624456
31	26.85458585735594	23.337950138504155	23.66594624212353	26.141517762016374
32	24.898785425101213	23.00234391647134	23.91860217344982	28.180268484977628
33	25.13774314328331	22.400383549968037	25.540318407354416	26.921554899394234
34	25.668929408541597	22.25579130011263	25.140787190648687	26.93449210069709
35	24.86453989224072	24.07841466013211	25.627834769109008	25.42921067851816
36	25.184164865605307	22.86612279687072	26.055523423944475	25.894188913579498
37	25.69099875194058	22.82655018112082	25.443669903503697	26.038781163434905
38	24.764847341024627	22.00313536878634	26.42309214331375	26.808925146875286
39	24.664393777967184	20.82356701470275	27.302821831907703	27.209217375422362
40	23.90642598398831	22.070865422665978	28.06839974430002	25.95430884904569
41	22.3653770052662	20.70561017929439	29.580530273051046	27.348482542388357
42	21.12492770387507	21.254299716903596	30.053118626525826	27.567653952695505
43	20.09375665885361	21.904203829411586	30.401662049861493	27.600377461873304
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	1.5
4	3.0
5	3.0
6	9.5
7	16.0
8	19.5
9	23.0
10	37.0
11	51.0
12	51.0
13	86.5
14	122.0
15	301.5
16	481.0
17	460.0
18	439.0
19	439.0
20	471.0
21	503.0
22	440.0
23	377.0
24	406.0
25	435.0
26	435.0
27	504.0
28	573.0
29	754.0
30	935.0
31	1153.5
32	1372.0
33	1372.0
34	1637.5
35	1903.0
36	2114.5
37	2326.0
38	2881.5
39	3437.0
40	3437.0
41	4075.0
42	4713.0
43	5320.5
44	5928.0
45	8272.5
46	10617.0
47	10617.0
48	11828.5
49	13040.0
50	14474.5
51	15909.0
52	15915.0
53	15921.0
54	15921.0
55	13849.5
56	11778.0
57	10967.0
58	10156.0
59	9412.5
60	8669.0
61	8669.0
62	7881.0
63	7093.0
64	5910.0
65	4727.0
66	4036.0
67	3345.0
68	3345.0
69	2836.5
70	2328.0
71	1975.5
72	1623.0
73	1289.0
74	955.0
75	955.0
76	808.0
77	661.0
78	540.0
79	419.0
80	316.5
81	214.0
82	214.0
83	181.0
84	148.0
85	109.0
86	70.0
87	61.5
88	53.0
89	53.0
90	39.5
91	26.0
92	18.5
93	11.0
94	6.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	131404.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.67011658701409
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.59949159026124	54.02270859334571
2	5.646803312067327	6.964780371982587
3	2.1163172378049535	3.915405923716173
4	1.0772856843170404	2.6574533499741255
5	0.7305304984143046	2.252595050378984
6	0.5121117514221898	1.8949194849471858
7	0.3627972407665634	1.5661623694864693
8	0.2542048693806533	1.2541475145353262
9	0.22212075965299802	1.2328391829776872
>10	1.3537026296629935	15.362546041216401
>50	0.08021027431913817	3.5394660740921133
>100	0.044424151930599606	5.336976043347234
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	371	0.28233539313871725	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	345	0.2625490852637667	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	342	0.26026604973973394	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	320	0.24352378923016044	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	300	0.22830355240327538	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	293	0.22297646951386563	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	256	0.19481903138412832	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	256	0.19481903138412832	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	255	0.1940580195427841	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	252	0.19177498401875134	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	242	0.18416486560530881	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	236	0.1795987945572433	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	236	0.1795987945572433	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	225	0.17122766430245653	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	203	0.15448540379288303	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	202	0.15372439195153875	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	188	0.14307022617271925	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	176	0.13393808407658822	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	168	0.12784998934583422	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	149	0.11339076436029344	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	146	0.11110772883626069	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG	142	0.10806368147088369	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	140	0.10654165778819519	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	139	0.10578064594685094	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	137	0.10425862226416244	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	7.610118413442513E-4	0.0
17	0.0	0.0	0.0	0.002283035524032754	0.0
18	0.0	0.0	0.0	0.0038050592067212564	0.0
19	0.0	0.0	0.0	0.005327082889409759	0.0
20	0.0	0.0	0.0	0.006088094730754011	0.0
21	0.0	0.0	0.0	0.008371130254786764	0.0
22	0.0	0.0	0.0	0.013698213144196524	0.0
23	0.0	0.0	0.0	0.01902529603360628	0.0
24	0.0	0.0	0.0	0.024352378923016043	0.0
25	0.0	0.0	0.0	0.0296794618124258	0.0
26	0.0	0.0	0.0	0.031201485495114305	0.0
27	0.0	0.0	0.0	0.047943746004687836	0.0
28	0.0	0.0	0.0	0.14307022617271925	0.0
29	0.0	0.0	0.0	0.34245532860491307	0.0
30	0.0	0.0	0.0	0.5547776323399592	0.0
31	0.0	0.0	0.0	1.2183799579921464	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTATA	20	0.0018360103	37.0	1
TATACAC	20	0.0018360103	37.0	37
GGTATCA	215	0.0	33.55814	1
TTTATAA	30	3.5823064E-4	30.833334	2
CTTATAC	135	0.0	26.037037	37
AGTCGGT	60	1.3224071E-6	24.666668	11
TGACCGA	105	1.8189894E-12	24.666666	24
AAGGCTA	100	2.0008883E-11	24.050001	5
CCGATAG	110	3.6379788E-12	23.545454	27
GACCGAT	110	3.6379788E-12	23.545454	25
ACCGATA	110	3.6379788E-12	23.545454	26
GACTGAC	110	3.6379788E-12	23.545454	21
CGATAGT	110	3.6379788E-12	23.545454	28
GCAGTCG	65	2.652945E-6	22.76923	9
ACTGACC	115	5.456968E-12	22.52174	22
TAGTGAA	115	5.456968E-12	22.52174	31
CCTGACT	115	5.456968E-12	22.52174	18
TGACTGA	115	5.456968E-12	22.52174	20
CTCTCTA	50	2.6844948E-4	22.2	22
GCCTTAT	60	3.6940597E-5	21.583334	26
>>END_MODULE