FastQCFastQC Report
Fri 10 Feb 2017
ERR1631945.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631945.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740564
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA14440.194986523784575No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA14230.19215084719214007No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA13890.18755975175676917No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA13590.1835087851961478No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA11700.1579876958642332No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG11550.1559622125839225No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11240.15177621380461379No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC10680.14421440955812057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10490.14164879740306036No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT10310.13921821746668755No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10180.13746279862375163No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9910.1338169287191924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9520.12855067219038463No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT9220.12449970562976326No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9110.1230143512242021No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7520.10154422845290888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAGT303.5982535E-430.8333324
GGTATCA10350.028.5990351
CAGTCGG2750.021.52727310
GCAGTCG2800.021.1428599
TCGTGTA705.0978797E-621.14285913
AACGACC1059.804353E-1021.14285737
ATACGGC909.46693E-820.55555529
GCTAAAC450.003824542320.5555553
ATACACA1550.020.29032337
TAGCAAT656.897894E-519.92307718
GTTTAGG759.258472E-619.7333341
AGTCGGT3000.019.11666711
CGTTATT500.00703257618.510
TGTTACG500.00703257618.516
GATGCTA609.2325883E-418.514
TTATTCA500.00703257618.54
GTATCAA16200.018.2716052
GTGATTC2900.017.86206816
TCCTCGC2300.017.69565221
GCCTAGT1054.7915637E-717.6190471
Produced by FastQC (version 0.11.5)