##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631945.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	740564
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54257970951869	34.0	31.0	34.0	31.0	34.0
2	32.71478899865508	34.0	31.0	34.0	31.0	34.0
3	32.80121096893719	34.0	31.0	34.0	31.0	34.0
4	36.24209116295148	37.0	37.0	37.0	35.0	37.0
5	36.15726662381644	37.0	35.0	37.0	35.0	37.0
6	36.21467557159138	37.0	36.0	37.0	35.0	37.0
7	36.205223316283266	37.0	36.0	37.0	35.0	37.0
8	36.181846538584104	37.0	36.0	37.0	35.0	37.0
9	38.006532858740094	39.0	38.0	39.0	35.0	39.0
10	37.93488071253801	39.0	38.0	39.0	35.0	39.0
11	38.0087136290719	39.0	38.0	39.0	35.0	39.0
12	37.94179031116825	39.0	38.0	39.0	35.0	39.0
13	38.00002970708811	39.0	38.0	39.0	35.0	39.0
14	39.3762132644849	40.0	39.0	41.0	37.0	41.0
15	39.3762132644849	40.0	39.0	41.0	36.0	41.0
16	39.32444461248454	40.0	39.0	41.0	36.0	41.0
17	39.295852890499674	40.0	39.0	41.0	36.0	41.0
18	39.313605576290506	40.0	39.0	41.0	36.0	41.0
19	39.33893627019407	40.0	39.0	41.0	36.0	41.0
20	39.337615655095306	40.0	39.0	41.0	36.0	41.0
21	39.30124202634749	40.0	39.0	41.0	36.0	41.0
22	39.28082245423758	40.0	39.0	41.0	36.0	41.0
23	39.213450829367886	40.0	39.0	41.0	36.0	41.0
24	39.23085243139013	40.0	39.0	41.0	36.0	41.0
25	39.171847402790306	40.0	39.0	41.0	36.0	41.0
26	39.1039626554896	40.0	39.0	41.0	36.0	41.0
27	38.998914340961754	40.0	39.0	41.0	35.0	41.0
28	38.989787513300676	40.0	39.0	41.0	35.0	41.0
29	38.973549889003515	40.0	38.0	41.0	35.0	41.0
30	38.931359072274645	40.0	38.0	41.0	35.0	41.0
31	38.84972939543375	40.0	38.0	41.0	35.0	41.0
32	38.79643758000659	40.0	38.0	41.0	35.0	41.0
33	38.74077864978584	40.0	38.0	41.0	35.0	41.0
34	38.71096623654404	40.0	38.0	41.0	35.0	41.0
35	38.64961839894999	40.0	38.0	41.0	35.0	41.0
36	38.602254497923205	40.0	38.0	41.0	35.0	41.0
37	38.561397799514964	40.0	38.0	41.0	35.0	41.0
38	38.513757082439874	40.0	38.0	41.0	35.0	41.0
39	38.44344985713591	40.0	38.0	41.0	34.0	41.0
40	38.15456732976488	40.0	38.0	41.0	34.0	41.0
41	38.22029156156659	40.0	37.0	41.0	34.0	41.0
42	38.20093334269557	40.0	37.0	41.0	34.0	41.0
43	37.72744151754608	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	6.0
18	5.0
19	12.0
20	26.0
21	53.0
22	113.0
23	217.0
24	374.0
25	633.0
26	1070.0
27	1595.0
28	2608.0
29	3668.0
30	5270.0
31	7239.0
32	9591.0
33	13476.0
34	19214.0
35	28510.0
36	44603.0
37	80220.0
38	184654.0
39	337403.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.60482551136701	16.873355982737483	13.034254973236614	27.487563532658893
2	20.10494704036383	20.065517632507117	33.58602362523698	26.243511701892068
3	20.92756331660734	20.440637136020655	29.759075515417976	28.87272403195402
4	15.347491911570103	16.35982845506938	34.281169487039605	34.01151014632091
5	16.277863898326142	33.58858923739204	33.81436310703734	16.319183757244478
6	33.32122544439103	34.09685050853133	15.907875619122722	16.67404842795491
7	29.72666778293301	28.458040088365085	20.494244926839546	21.321047201862363
8	26.4518664153267	31.752556159899754	20.521926531670456	21.273650893103095
9	27.871325098168427	13.567902301489134	18.286468151300898	40.274304449041544
10	18.85414359866264	24.377501471851186	30.508234264695556	26.26012066479062
11	37.674124045997374	20.437531394990845	19.725911602508358	22.162432956503423
12	22.199971913298512	24.471348863838912	27.275157852663646	26.05352137019893
13	33.63598554615131	18.378965221101755	22.037663186436284	25.94738604631065
14	23.666151743806072	20.625631275622364	23.686946705483926	32.02127027508764
15	28.703528661938737	25.140568539653565	20.79550180673109	25.360400991676613
16	25.137732863061125	24.943151435932613	22.996932067991423	26.92218363301484
17	25.988570873010303	24.698743120108457	22.57050032137668	26.74218568550456
18	25.668949611377272	22.53134097795734	24.397351207998227	27.402358202667155
19	27.26124953413884	23.245661414813572	24.260968667123976	25.232120383923608
20	28.566200895533672	21.883996521570044	23.992524616373466	25.55727796652281
21	26.465369637195437	23.316283265187074	22.956962531259958	27.26138456635753
22	27.032504955682423	23.683705932235434	22.521213561555786	26.762575550526357
23	26.569074381147338	23.43389632766378	23.44523903403352	26.551790257155357
24	27.145121826067697	22.768727618409752	23.676819289082378	26.409331266440173
25	27.109068223678168	22.816529023825087	23.961737270512746	26.112665481984
26	26.877623000848	23.548268616891992	23.304265397723896	26.269842984536112
27	26.318724647700943	22.66947893767453	24.50132601638751	26.51047039823702
28	25.469101927719954	23.73015701546389	23.347205643266484	27.453535413549673
29	26.318454583263566	24.265829826996722	23.27010224639599	26.145613343343722
30	26.019898347745773	23.371916539286275	24.35751670348545	26.250668409482504
31	26.980247487050413	23.237424449473647	23.69666902522942	26.08565903824653
32	24.81081986161898	23.294948174634467	24.024392219983685	27.869839743762864
33	25.15056092383643	22.878778876639966	25.298691267736483	26.671968931787127
34	25.52662565288078	22.931441441928044	24.69766286235896	26.844270042832218
35	25.21443116327556	24.113378452098672	24.912634154509266	25.759556230116505
36	25.48733127724275	23.157215311573342	25.41427884693288	25.94117456425103
37	26.218530741434908	22.44789106680854	24.93666988943562	26.39690830232093
38	25.24359812251203	22.225087905974366	25.943605144187405	26.5877088273262
39	24.828644114485716	21.45769980717399	26.855882813639337	26.857773264700956
40	24.710220858696886	22.03887847640447	27.465013152138102	25.785887512760546
41	23.10671326178426	21.658357684143436	28.33097477058026	26.903954283492045
42	22.18930436802221	21.95029734094555	28.611976817668694	27.248421473363543
43	21.35115938662965	22.697565639161503	28.596448112519646	27.354826861689197
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9.0
1	8.5
2	8.0
3	25.0
4	42.0
5	42.0
6	55.5
7	69.0
8	81.5
9	94.0
10	151.5
11	209.0
12	209.0
13	410.5
14	612.0
15	1254.0
16	1896.0
17	1886.5
18	1877.0
19	1877.0
20	2160.0
21	2443.0
22	2281.0
23	2119.0
24	2391.0
25	2663.0
26	2663.0
27	3061.0
28	3459.0
29	4985.0
30	6511.0
31	7450.5
32	8390.0
33	8390.0
34	10482.0
35	12574.0
36	13865.0
37	15156.0
38	19377.5
39	23599.0
40	23599.0
41	27668.5
42	31738.0
43	34570.0
44	37402.0
45	47821.5
46	58241.0
47	58241.0
48	63912.5
49	69584.0
50	77626.5
51	85669.0
52	86833.5
53	87998.0
54	87998.0
55	77262.0
56	66526.0
57	61156.5
58	55787.0
59	50791.0
60	45795.0
61	45795.0
62	41739.0
63	37683.0
64	31429.5
65	25176.0
66	21785.0
67	18394.0
68	18394.0
69	15891.0
70	13388.0
71	11527.0
72	9666.0
73	7840.0
74	6014.0
75	6014.0
76	4986.5
77	3959.0
78	3277.5
79	2596.0
80	2039.5
81	1483.0
82	1483.0
83	1190.5
84	898.0
85	666.0
86	434.0
87	329.0
88	224.0
89	224.0
90	182.0
91	140.0
92	84.0
93	28.0
94	19.0
95	10.0
96	10.0
97	5.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	740564.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.41237092255537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.80550587002477	39.82436965637092
2	6.8336684927971	6.343335136989578
3	2.1931790792760664	3.0537192278084784
4	1.1184067831160203	2.0763164184113068
5	0.6856390240566733	1.5911066351748593
6	0.46656070937092553	1.299251322072837
7	0.3556273770150534	1.155385681256667
8	0.27104361848914077	1.0063821566007671
9	0.2025929661724738	0.846253790306781
>10	1.6536346480210387	15.801096525810424
>50	0.24041262030474264	7.998145448748444
>100	0.15852022854369865	13.681998214188647
>500	0.01199138260201367	3.5267528062745233
>1k	0.0032172002102963506	1.7958869799858335
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1444	0.194986523784575	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1423	0.19215084719214007	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1389	0.18755975175676917	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1359	0.1835087851961478	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1170	0.1579876958642332	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1155	0.1559622125839225	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1124	0.15177621380461379	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1068	0.14421440955812057	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1049	0.14164879740306036	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1031	0.13921821746668755	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1018	0.13746279862375163	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	991	0.1338169287191924	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	952	0.12855067219038463	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	922	0.12449970562976326	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	911	0.1230143512242021	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	752	0.10154422845290888	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.350322186873788E-4	0.0
10	2.700644373747576E-4	0.0	0.0	1.350322186873788E-4	0.0
11	2.700644373747576E-4	0.0	0.0	1.350322186873788E-4	0.0
12	2.700644373747576E-4	0.0	0.0	1.350322186873788E-4	0.0
13	2.700644373747576E-4	0.0	0.0	1.350322186873788E-4	0.0
14	2.700644373747576E-4	0.0	0.0	1.350322186873788E-4	0.0
15	4.0509665606213645E-4	0.0	0.0	2.700644373747576E-4	0.0
16	4.0509665606213645E-4	0.0	0.0	5.401288747495152E-4	0.0
17	4.0509665606213645E-4	0.0	0.0	8.101933121242729E-4	0.0
18	4.0509665606213645E-4	0.0	0.0	0.0010802577494990304	0.0
19	4.0509665606213645E-4	0.0	0.0	0.0016203866242485458	0.0
20	4.0509665606213645E-4	0.0	0.0	0.0021605154989980608	0.0
21	4.0509665606213645E-4	0.0	0.0	0.0031057410298097124	0.0
22	4.0509665606213645E-4	0.0	0.0	0.0037809021232466065	0.0
23	4.0509665606213645E-4	0.0	0.0	0.005266256528807773	0.0
24	4.0509665606213645E-4	0.0	0.0	0.0066165787156815615	0.0
25	4.0509665606213645E-4	0.0	0.0	0.007291739809118456	0.0
26	5.401288747495152E-4	0.0	0.0	0.008642061995992243	0.0
27	5.401288747495152E-4	0.0	0.0	0.025926185987976733	0.0
28	5.401288747495152E-4	0.0	0.0	0.1031646150771574	0.0
29	5.401288747495152E-4	0.0	0.0	0.2184821298361789	0.0
30	5.401288747495152E-4	0.0	0.0	0.3809258889170956	0.0
31	5.401288747495152E-4	0.0	0.0	0.8380099491738728	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGAGT	30	3.5982535E-4	30.833332	4
GGTATCA	1035	0.0	28.599035	1
CAGTCGG	275	0.0	21.527273	10
GCAGTCG	280	0.0	21.142859	9
TCGTGTA	70	5.0978797E-6	21.142859	13
AACGACC	105	9.804353E-10	21.142857	37
ATACGGC	90	9.46693E-8	20.555555	29
GCTAAAC	45	0.0038245423	20.555555	3
ATACACA	155	0.0	20.290323	37
TAGCAAT	65	6.897894E-5	19.923077	18
GTTTAGG	75	9.258472E-6	19.733334	1
AGTCGGT	300	0.0	19.116667	11
CGTTATT	50	0.007032576	18.5	10
TGTTACG	50	0.007032576	18.5	16
GATGCTA	60	9.2325883E-4	18.5	14
TTATTCA	50	0.007032576	18.5	4
GTATCAA	1620	0.0	18.271605	2
GTGATTC	290	0.0	17.862068	16
TCCTCGC	230	0.0	17.695652	21
GCCTAGT	105	4.7915637E-7	17.619047	1
>>END_MODULE