FastQCFastQC Report
Fri 10 Feb 2017
ERR1631944.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631944.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750085
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA12830.17104728130811842No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA12280.16371477899171427No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12140.1618483238566296No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11670.15558236733170241No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10260.13678449775692086No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9890.13185172347133992No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9540.1271855856336282No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9270.12358599358739343No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9200.12265276601985109No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC8560.11412039968803536No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT8320.11092076231360445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8280.11038748941786598No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8200.10932094362638901No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7970.10625462447589272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7650.030.2287581
ATCCGAT250.005495141729.59999821
CCGCTTA358.8665116E-426.4285725
ATAACGC705.098025E-621.14285735
CGGCATA450.003824587720.55555521
TTATGCA450.003824587720.5555554
TCAATAC555.140805E-420.1818183
ATCCTAG656.898045E-519.9230771
TTATACA4200.018.94047737
ATCTAAT701.2184809E-418.51
CTAATCC1205.164111E-918.53
CTAGACA500.0070326618.4999984
TATCCCG500.0070326618.4999985
GTATCAA12400.018.3508072
CTTATAC7900.017.56329237
ATACGGC852.7210725E-517.41176429
TCTAATC752.0660603E-417.2666662
GCGGTAA5100.017.04901923
GGTAATA5400.016.44444525
GTATTAG1352.2155291E-816.4444451
Produced by FastQC (version 0.11.5)