##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631944.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	750085
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56331482431991	34.0	31.0	34.0	31.0	34.0
2	32.731418439243555	34.0	31.0	34.0	31.0	34.0
3	32.83281094809255	34.0	31.0	34.0	31.0	34.0
4	36.259753227967494	37.0	37.0	37.0	35.0	37.0
5	36.18016358146077	37.0	35.0	37.0	35.0	37.0
6	36.22677963164175	37.0	36.0	37.0	35.0	37.0
7	36.21242259210623	37.0	36.0	37.0	35.0	37.0
8	36.194865915196274	37.0	36.0	37.0	35.0	37.0
9	38.02983128578761	39.0	38.0	39.0	35.0	39.0
10	37.950980222241476	39.0	38.0	39.0	35.0	39.0
11	38.03553730577201	39.0	38.0	39.0	35.0	39.0
12	37.96250691588287	39.0	38.0	39.0	35.0	39.0
13	38.024774525553774	39.0	38.0	39.0	35.0	39.0
14	39.40124919175827	40.0	39.0	41.0	37.0	41.0
15	39.40434350773579	40.0	39.0	41.0	37.0	41.0
16	39.36216695441183	40.0	39.0	41.0	36.0	41.0
17	39.324484558416714	40.0	39.0	41.0	36.0	41.0
18	39.34317444023011	40.0	39.0	41.0	36.0	41.0
19	39.36423738642954	40.0	39.0	41.0	36.0	41.0
20	39.34832718958518	40.0	39.0	41.0	36.0	41.0
21	39.31831459101302	40.0	39.0	41.0	36.0	41.0
22	39.29811688008692	40.0	39.0	41.0	36.0	41.0
23	39.22809814887646	40.0	39.0	41.0	36.0	41.0
24	39.24549084437097	40.0	39.0	41.0	36.0	41.0
25	39.18639620842971	40.0	39.0	41.0	36.0	41.0
26	39.12777618536566	40.0	39.0	41.0	36.0	41.0
27	39.02502516381477	40.0	39.0	41.0	35.0	41.0
28	39.013021190931696	40.0	39.0	41.0	35.0	41.0
29	38.97513348487171	40.0	39.0	41.0	35.0	41.0
30	38.9433570861969	40.0	38.0	41.0	35.0	41.0
31	38.86513261830326	40.0	38.0	41.0	35.0	41.0
32	38.812818547231316	40.0	38.0	41.0	35.0	41.0
33	38.74752994660605	40.0	38.0	41.0	35.0	41.0
34	38.71561889652506	40.0	38.0	41.0	35.0	41.0
35	38.64981435437317	40.0	38.0	41.0	35.0	41.0
36	38.60436483865162	40.0	38.0	41.0	35.0	41.0
37	38.55817807315171	40.0	38.0	41.0	35.0	41.0
38	38.51970110054194	40.0	38.0	41.0	35.0	41.0
39	38.4547724591213	40.0	38.0	41.0	34.0	41.0
40	38.160035196011115	40.0	37.0	41.0	34.0	41.0
41	38.21828592759487	40.0	37.0	41.0	34.0	41.0
42	38.19793356752901	40.0	37.0	41.0	34.0	41.0
43	37.733126245692155	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	4.0
19	8.0
20	13.0
21	43.0
22	94.0
23	190.0
24	362.0
25	577.0
26	1016.0
27	1684.0
28	2478.0
29	3634.0
30	5198.0
31	7267.0
32	9748.0
33	13435.0
34	19544.0
35	29233.0
36	45366.0
37	81071.0
38	182180.0
39	346936.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.1584420432351	16.633981482098694	12.379130365225274	27.82844610944093
2	20.580734183459207	19.422465453915223	32.527246912016636	27.46955345060893
3	20.900031329782625	19.98040222108161	29.007912436590523	30.111654012545248
4	15.251204863448809	15.739816154169194	33.90789043908357	35.101088543298424
5	16.92208216402141	32.88453975216142	33.299292746822026	16.89408533699514
6	34.794323310024865	33.68364918642554	15.288000693254764	16.234026810294832
7	30.93089449862349	27.7940500076658	19.91467633668184	21.36037915702887
8	27.774052274075604	30.86636847823913	19.733363552130758	21.626215695554503
9	28.22306805228741	12.920402354399835	17.823846630715185	41.03268296259757
10	18.970649993000794	23.810368158275395	30.338961584353775	26.880020264370035
11	39.00891232327003	19.58778005159415	19.290747048667818	22.112560576468002
12	23.086450202310406	23.805168747541945	26.944146330082592	26.16423472006506
13	34.56474932840944	17.751721471566555	21.052014105068093	26.631515094955905
14	23.99194757927435	19.65350593599392	23.3274895511842	33.027056933547534
15	28.883793170107385	24.449495723817968	20.149316410806776	26.51739469526787
16	25.730950492277543	24.128332122359467	22.464654005879332	27.676063379483658
17	26.39740829372671	23.8384983035256	22.389329209356273	27.374764193391414
18	26.69404134198124	22.104428164807988	23.44814254384503	27.753387949365738
19	27.80338228334122	22.71609217622003	23.377083930487878	26.103441609950874
20	28.95791810261504	21.737936367211717	22.88607291173667	26.41807261843658
21	27.389162561576352	22.657832112360598	22.292673497003673	27.660331829059377
22	27.985494977235913	23.1653745908797	21.790997020337695	27.05813341154669
23	27.6661978309125	22.50371624549218	22.688895258537368	27.141190665057962
24	27.43542398528167	22.17028736743169	22.772352466720438	27.621936180566202
25	27.42502516381477	22.37973029723298	23.20723651319517	26.988008025757082
26	27.285041028683416	22.994460627795515	22.76435337328436	26.956144970236707
27	26.686842157888773	22.40626062379597	23.764773325689756	27.142123892625502
28	26.43220435017365	23.040322096829026	22.652766019851082	27.874707533146243
29	26.925081824059937	23.522667431024484	22.778885059693234	26.773365685222338
30	26.802962330935827	22.367198384183126	23.60079191025017	27.229047374630877
31	27.353033322890074	22.740489411200063	23.011125405787343	26.895351860122517
32	25.682289340541402	22.76022050834239	23.244965570568667	28.31252458054754
33	25.75361459034643	22.434657405494043	24.558283394548617	27.25344460961091
34	26.246092109560916	22.299606044648275	24.011678676416672	27.442623169374137
35	25.577901171200594	23.525867068398913	24.617743322423458	26.27848843797703
36	26.027850176979943	22.37653065985855	24.820387022804084	26.775232140357424
37	26.030249905010766	22.158288727277576	24.796123106047983	27.01533826166368
38	25.35752614703667	21.687408760340496	25.673357019537786	27.28170807308505
39	24.8774472226481	21.032816280821507	26.529659971869858	27.560076524660538
40	24.904377503882895	21.624749195091223	27.10626129038709	26.364612010638794
41	23.545198210869433	21.10014198390849	28.05002099762027	27.304638807601805
42	22.286140904030876	21.655945659491923	28.58542698494171	27.472486451535495
43	21.683942486518195	21.792196884353107	28.587426758300726	27.93643387082797
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	6.0
2	7.0
3	17.5
4	28.0
5	28.0
6	40.5
7	53.0
8	61.5
9	70.0
10	117.5
11	165.0
12	165.0
13	327.5
14	490.0
15	961.0
16	1432.0
17	1425.5
18	1419.0
19	1419.0
20	1713.5
21	2008.0
22	1803.5
23	1599.0
24	1937.5
25	2276.0
26	2276.0
27	2791.0
28	3306.0
29	4283.0
30	5260.0
31	6420.5
32	7581.0
33	7581.0
34	9636.0
35	11691.0
36	13646.0
37	15601.0
38	18460.0
39	21319.0
40	21319.0
41	24959.0
42	28599.0
43	33088.5
44	37578.0
45	46367.5
46	55157.0
47	55157.0
48	59504.5
49	63852.0
50	70424.5
51	76997.0
52	77074.5
53	77152.0
54	77152.0
55	71719.0
56	66286.0
57	63199.5
58	60113.0
59	57454.5
60	54796.0
61	54796.0
62	50401.0
63	46006.0
64	39561.5
65	33117.0
66	29027.5
67	24938.0
68	24938.0
69	21541.5
70	18145.0
71	15372.0
72	12599.0
73	10153.5
74	7708.0
75	7708.0
76	6420.5
77	5133.0
78	4176.5
79	3220.0
80	2620.5
81	2021.0
82	2021.0
83	1585.0
84	1149.0
85	886.5
86	624.0
87	490.0
88	356.0
89	356.0
90	264.5
91	173.0
92	107.0
93	41.0
94	26.5
95	12.0
96	12.0
97	7.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	750085.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.78521268482277
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.19607061046254	53.610169100226116
2	6.421652230376027	7.8068299322274655
3	1.8554432238605885	3.383505329609373
4	0.8934085508084654	2.1722411510132744
5	0.5478119733847417	1.6649433656741996
6	0.34353609782847594	1.2529148862850792
7	0.26562844492090687	1.1302397063759237
8	0.19561745459376212	0.9512518865876394
9	0.1430611047059875	0.7826399708831234
>10	0.9596419425525365	11.365300225295934
>50	0.10238548008219732	4.40932817829165
>100	0.06869703652434508	8.239906568489669
>500	0.0059449358527514096	2.438672929542215
>1k	0.0011009140468058165	0.7920567694984266
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1283	0.17104728130811842	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1228	0.16371477899171427	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1214	0.1618483238566296	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1167	0.15558236733170241	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1026	0.13678449775692086	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	989	0.13185172347133992	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	954	0.1271855856336282	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	927	0.12358599358739343	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	920	0.12265276601985109	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	856	0.11412039968803536	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	832	0.11092076231360445	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	828	0.11038748941786598	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	820	0.10932094362638901	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	797	0.10625462447589272	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3331822393462074E-4	0.0	0.0	0.0
3	0.0	1.3331822393462074E-4	0.0	0.0	0.0
4	0.0	1.3331822393462074E-4	0.0	0.0	0.0
5	0.0	1.3331822393462074E-4	0.0	0.0	0.0
6	0.0	1.3331822393462074E-4	0.0	0.0	0.0
7	0.0	1.3331822393462074E-4	0.0	0.0	0.0
8	1.3331822393462074E-4	1.3331822393462074E-4	0.0	0.0	0.0
9	1.3331822393462074E-4	1.3331822393462074E-4	0.0	0.0	0.0
10	2.666364478692415E-4	1.3331822393462074E-4	0.0	0.0	0.0
11	2.666364478692415E-4	1.3331822393462074E-4	0.0	0.0	0.0
12	2.666364478692415E-4	1.3331822393462074E-4	0.0	0.0	0.0
13	2.666364478692415E-4	1.3331822393462074E-4	0.0	0.0	0.0
14	2.666364478692415E-4	1.3331822393462074E-4	0.0	0.0	0.0
15	2.666364478692415E-4	1.3331822393462074E-4	0.0	2.666364478692415E-4	0.0
16	2.666364478692415E-4	1.3331822393462074E-4	0.0	3.999546718038622E-4	0.0
17	2.666364478692415E-4	1.3331822393462074E-4	0.0	7.999093436077244E-4	0.0
18	2.666364478692415E-4	1.3331822393462074E-4	0.0	0.001066545791476966	0.0
19	2.666364478692415E-4	1.3331822393462074E-4	0.0	0.0011998640154115866	0.0
20	2.666364478692415E-4	1.3331822393462074E-4	0.0	0.002533046254757794	0.0
21	2.666364478692415E-4	1.3331822393462074E-4	0.0	0.003999546718038622	0.0
22	2.666364478692415E-4	1.3331822393462074E-4	0.0	0.0058660018531233125	0.0
23	2.666364478692415E-4	1.3331822393462074E-4	0.0	0.006799229420665658	0.0
24	2.666364478692415E-4	1.3331822393462074E-4	0.0	0.008265729883946487	0.0
25	2.666364478692415E-4	1.3331822393462074E-4	0.0	0.008932321003619589	0.0
26	2.666364478692415E-4	1.3331822393462074E-4	0.0	0.011732003706246625	0.0
27	2.666364478692415E-4	1.3331822393462074E-4	0.0	0.03239632841611284	0.0
28	2.666364478692415E-4	1.3331822393462074E-4	0.0	0.11518694547951232	0.0
29	2.666364478692415E-4	1.3331822393462074E-4	0.0	0.2449055773678983	0.0
30	2.666364478692415E-4	1.3331822393462074E-4	0.0	0.41781931381110143	0.0
31	2.666364478692415E-4	1.3331822393462074E-4	0.0	0.8982981928714746	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	765	0.0	30.228758	1
ATCCGAT	25	0.0054951417	29.599998	21
CCGCTTA	35	8.8665116E-4	26.42857	25
ATAACGC	70	5.098025E-6	21.142857	35
CGGCATA	45	0.0038245877	20.555555	21
TTATGCA	45	0.0038245877	20.555555	4
TCAATAC	55	5.140805E-4	20.181818	3
ATCCTAG	65	6.898045E-5	19.923077	1
TTATACA	420	0.0	18.940477	37
ATCTAAT	70	1.2184809E-4	18.5	1
CTAATCC	120	5.164111E-9	18.5	3
CTAGACA	50	0.00703266	18.499998	4
TATCCCG	50	0.00703266	18.499998	5
GTATCAA	1240	0.0	18.350807	2
CTTATAC	790	0.0	17.563292	37
ATACGGC	85	2.7210725E-5	17.411764	29
TCTAATC	75	2.0660603E-4	17.266666	2
GCGGTAA	510	0.0	17.049019	23
GGTAATA	540	0.0	16.444445	25
GTATTAG	135	2.2155291E-8	16.444445	1
>>END_MODULE