FastQCFastQC Report
Fri 10 Feb 2017
ERR1631943.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631943.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114966
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6570.5714733051510881No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6150.5349407650957675No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5210.45317746116243063No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4740.41229580919576225No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4550.395769183932641No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3980.34618930814327714No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3800.3305325052624254No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3560.30965676808795645No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3340.2905206756780266No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3290.28617156376667885No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3250.2826922742376007No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3170.27573369517944435No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3160.27486387279717484No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3090.268775116121288No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2960.257467425151784No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2860.24876920132908859No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2860.24876920132908859No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2840.24702955656454953No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2680.23311239844823686No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2670.23224257606596732No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2560.2226745298610024No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2520.21919524033192422No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2430.21136683889149835No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2430.21136683889149835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2380.20701772698015064No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC2370.20614790459788113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.20353843745107247No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2320.20179879268653342No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2230.19397039124610754No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2220.193100568863838No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC2200.19136092409929892No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2190.19049110171702938No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2190.19049110171702938No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2160.18788163457022078No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT2160.18788163457022078No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2140.1861419898056817No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2140.1861419898056817No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2140.1861419898056817No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2120.1844023450411426No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2110.18353252265887304No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2080.18092305551206445No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA2060.17918341074752536No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC2010.17483429883617765No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2010.17483429883617765No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1980.17222483168936903No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA1920.16700589739575178No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1900.1652662526312127No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA1880.16352660786667364No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1880.16352660786667364No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA1870.16265678548440407No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1830.1591774959553259No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1820.1583076735730564No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC1820.1583076735730564No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1810.15743785119078685No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1750.1522189168971696No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1700.1478698049858219No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC1660.14439051545674372No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1620.14091122592766556No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA1620.14091122592766556No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA1620.14091122592766556No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC1550.13482246925177876No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1510.13134317972270063No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG1490.12960353495816154No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT1470.12786389019362246No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC1450.12612424542908338No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT1430.12438460066454429No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1430.12438460066454429No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC1420.12351477828227476No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1400.12177513351773568No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC1370.11916566637092706No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC1360.11829584398865751No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC1340.11655619922411843No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT1300.11307690969504028No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG1300.11307690969504028No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA1290.11220708731277072No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA1270.11046744254823164No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT1260.10959762016596211No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG1230.1069881530191535No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC1220.10611833063688395No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC1210.1052485082546144No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC1210.1052485082546144No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG1200.10437868587234486No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT1200.10437868587234486No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGC1200.10437868587234486No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG1200.10437868587234486No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA1190.10350886349007532No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT1180.10263904110780579No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA1180.10263904110780579No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1170.10176921872553624No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA1170.10176921872553624No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG1150.10002957396099715No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCGG251.2267263E-437.010
CCGTGAC200.001835054437.037
ATACGGC401.583543E-632.37529
TGGGCAG303.5795374E-430.8333326
TTCACGA250.005476042629.62
CGTTATT250.005476042629.610
CCTCGTA250.005476042629.630
TATTGTT250.005476042629.613
TGTTACG250.005476042629.616
ACACAGC250.005476042629.61
TCACGAC250.005476042629.63
ATTGTTA250.005476042629.614
AGTGTCA250.005476042629.627
CGTAAAA250.005476042629.633
TCGTAAA250.005476042629.632
AATGTTC250.005476042629.620
TTCAGTG250.005476042629.624
GTTATTG250.005476042629.611
GCCGACC250.005476042629.632
CTCGTAA250.005476042629.631
Produced by FastQC (version 0.11.5)