FastQCFastQC Report
Fri 10 Feb 2017
ERR1631942.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631942.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804676
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13330.16565673637588296No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC13300.1652839155138217No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA13260.16478682103107337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13200.16404117930695086No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA13130.1631712639621413No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA12630.15695758292778708No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11870.1475127877555687No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA11050.1373223508592278No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10880.13520969930754737No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9950.12365225258364858No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9830.12216096913540357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9800.12178814827334232No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT9540.11855703413547813No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC8890.11047924879081768No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA8800.10936078620463392No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8760.10886369172188558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11050.029.9683251
ATACGGC604.3193722E-827.75000229
TTTTACG551.9017387E-523.5454564
CTAGACC400.001930686823.1253
AATACGC400.001930686823.1255
TTACGAG603.7244972E-521.5833346
GACAGGC4050.021.4691357
GTACTAT705.0987874E-621.1428571
GCGGTAA5400.020.8981523
TAGACCA450.00382482620.5555554
ACCGCTC450.00382482620.5555558
CTTATAC9250.020.037
GACCGTT656.898845E-519.9230777
CATCAGA4200.019.3809516
GTATCAA17200.019.2529072
CGGTAAT5950.018.96638724
GGACAGG4750.018.6947366
CCTAGAC609.233531E-418.52
GATTTAT500.007033091518.51
GGACGTA609.233531E-418.527
Produced by FastQC (version 0.11.5)