##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631942.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	804676
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36438516868901	33.0	31.0	34.0	31.0	34.0
2	32.54701768165075	34.0	31.0	34.0	31.0	34.0
3	32.631413140195555	34.0	31.0	34.0	31.0	34.0
4	36.135607374893745	37.0	35.0	37.0	35.0	37.0
5	36.02699471588565	37.0	35.0	37.0	35.0	37.0
6	36.07428828497432	37.0	35.0	37.0	35.0	37.0
7	36.06925271786408	37.0	35.0	37.0	35.0	37.0
8	36.043402562024966	37.0	35.0	37.0	35.0	37.0
9	37.850963617654806	39.0	38.0	39.0	35.0	39.0
10	37.75456208461542	39.0	37.0	39.0	35.0	39.0
11	37.83664108287062	39.0	38.0	39.0	35.0	39.0
12	37.76026003012392	39.0	37.0	39.0	35.0	39.0
13	37.812609050102154	39.0	38.0	39.0	35.0	39.0
14	39.14412757432805	40.0	39.0	41.0	36.0	41.0
15	39.134691478309286	40.0	39.0	41.0	36.0	41.0
16	39.08536230731375	40.0	38.0	41.0	36.0	41.0
17	39.04831634098693	40.0	38.0	41.0	36.0	41.0
18	39.06115629147632	40.0	38.0	41.0	36.0	41.0
19	39.0805417335673	40.0	39.0	41.0	36.0	41.0
20	39.064332725221085	40.0	39.0	41.0	36.0	41.0
21	39.031722084416586	40.0	39.0	41.0	36.0	41.0
22	38.99746108992936	40.0	38.0	41.0	36.0	41.0
23	38.935219889744445	40.0	38.0	41.0	35.0	41.0
24	38.944867250918385	40.0	38.0	41.0	35.0	41.0
25	38.877621552028394	40.0	38.0	41.0	35.0	41.0
26	38.795434435723195	40.0	38.0	41.0	35.0	41.0
27	38.689470047571945	40.0	38.0	41.0	35.0	41.0
28	38.67515248373258	40.0	38.0	41.0	35.0	41.0
29	38.647956444581425	40.0	38.0	41.0	35.0	41.0
30	38.59821965611004	40.0	38.0	41.0	35.0	41.0
31	38.52558172481844	40.0	38.0	41.0	35.0	41.0
32	38.474194582664325	40.0	38.0	41.0	34.0	41.0
33	38.404090590498534	40.0	38.0	41.0	34.0	41.0
34	38.36823392272169	40.0	38.0	41.0	34.0	41.0
35	38.27136636360473	40.0	38.0	41.0	34.0	41.0
36	38.23053626552799	40.0	38.0	41.0	34.0	41.0
37	38.188801455492644	40.0	38.0	41.0	34.0	41.0
38	38.15627159254159	40.0	37.0	41.0	34.0	41.0
39	38.09048859416709	40.0	37.0	41.0	33.0	41.0
40	37.774515457152944	40.0	37.0	41.0	33.0	41.0
41	37.83751596916026	40.0	37.0	41.0	33.0	41.0
42	37.80581376852298	40.0	37.0	41.0	33.0	41.0
43	37.305546580238506	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	3.0
17	3.0
18	10.0
19	7.0
20	40.0
21	90.0
22	147.0
23	292.0
24	546.0
25	869.0
26	1350.0
27	2252.0
28	3483.0
29	4891.0
30	6842.0
31	9365.0
32	12697.0
33	17420.0
34	25297.0
35	38026.0
36	57484.0
37	100434.0
38	225089.0
39	298036.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.100204305832406	16.68323648275828	12.530260626637304	27.686298584772008
2	20.698020072675213	19.110797389259776	33.469247249824775	26.721935288240235
3	21.086002316460288	20.303202779752347	29.013043759227315	29.597751144560046
4	15.448329513990725	15.75789510312225	34.021519220158176	34.77225616272885
5	16.815090794307274	33.11270623207353	33.30085649379377	16.771346479825418
6	34.24633019998111	33.932539307746225	15.264777376235902	16.556353116036764
7	30.653952646779576	27.86264285252698	19.92193131148437	21.561473189209075
8	27.423459877018825	31.28998503745607	19.64641669442111	21.640138391103996
9	28.38645616372304	13.062400270419399	17.74515457152941	40.80598899432815
10	19.686308526661662	23.598939200373813	29.628198181628385	27.08655409133614
11	38.62386848868364	19.969279560966154	19.102719603915116	22.304132346435086
12	23.564142586581433	23.80796743036949	26.377573085316325	26.250316897732752
13	33.722019794302305	17.921001744801636	21.44440743852184	26.91257102237422
14	23.89098220898846	19.808593769417755	23.67188781571713	32.62853620587665
15	29.144152429052188	24.04110474277846	20.82987438422421	25.984868443945143
16	25.44924913878381	24.082487858467257	22.357818550571906	28.110444452177024
17	26.30884977307637	24.09193265363948	22.44505863229424	27.154158940989916
18	26.032713787909668	21.960888606097363	23.935596438815125	28.070801167177844
19	27.870844911492327	22.322152021434714	23.636594107442	26.170408959630958
20	28.49469848734149	21.51772887472722	23.60564997589092	26.381922662040374
21	27.804482798045427	22.44406444332874	22.521486909016797	27.229965849609034
22	28.119889247349246	23.302173794173058	21.804055296790263	26.773881661687437
23	27.673249854599863	22.777366294011504	22.76630594177035	26.78307790961828
24	27.731782729943482	22.33557357246892	22.6811785115997	27.2514651859879
25	27.344670401503212	22.321282106089903	23.60092757830481	26.733119914102073
26	27.576316430463937	22.971108868662668	23.219780383657522	26.232794317215873
27	27.46422162460419	22.236651770402	23.51517878003072	26.78394782496309
28	26.100567184804817	22.945632776421814	23.251470156932726	27.702329881840644
29	27.212940363574905	23.223881413140194	23.273715135035715	26.28946308824918
30	26.527074251002887	22.17140811954128	24.4659962519076	26.835521377548233
31	27.019446336165114	22.605744423842637	23.464350869169703	26.910458370822543
32	25.13433978396274	22.54982129453345	23.87532373278189	28.44051518872192
33	25.708608185157754	22.58610919177408	24.8951130641401	26.810169558928067
34	26.321401408765766	22.38130626488177	24.608910915697745	26.68838141065472
35	25.997544353255226	23.483986101238262	24.58877858914644	25.929690956360073
36	26.231551581009004	22.335697846089605	24.66160293086907	26.77114764203232
37	25.651939414124442	22.03831107178541	25.032435414999327	27.277314099090816
38	25.65691035895193	21.171129746630942	26.00152110911721	27.170438785299922
39	24.391059258633287	20.949798428187247	27.114391382370044	27.54475093080942
40	24.565912242940016	21.399917482315864	27.899427844250358	26.134742430493763
41	23.184735222623765	20.67465663198604	28.626055704407737	27.51455244098246
42	21.83649071178959	21.7555885847223	28.916234608712077	27.491686094776036
43	21.43198007645313	21.31056474904185	29.079654419915595	28.177800754589427
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	14.5
2	16.0
3	35.0
4	54.0
5	54.0
6	74.5
7	95.0
8	107.0
9	119.0
10	177.0
11	235.0
12	235.0
13	549.0
14	863.0
15	1586.5
16	2310.0
17	2227.5
18	2145.0
19	2145.0
20	2469.5
21	2794.0
22	2256.0
23	1718.0
24	1835.0
25	1952.0
26	1952.0
27	2280.0
28	2608.0
29	3432.5
30	4257.0
31	5577.0
32	6897.0
33	6897.0
34	9655.5
35	12414.0
36	14496.5
37	16579.0
38	20898.0
39	25217.0
40	25217.0
41	28819.5
42	32422.0
43	38173.0
44	43924.0
45	51698.0
46	59472.0
47	59472.0
48	64555.0
49	69638.0
50	76948.0
51	84258.0
52	84947.5
53	85637.0
54	85637.0
55	79907.0
56	74177.0
57	68218.5
58	62260.0
59	57720.5
60	53181.0
61	53181.0
62	50355.5
63	47530.0
64	38602.5
65	29675.0
66	26086.0
67	22497.0
68	22497.0
69	19421.5
70	16346.0
71	14724.0
72	13102.0
73	13282.0
74	13462.0
75	13462.0
76	11546.0
77	9630.0
78	6741.0
79	3852.0
80	2671.0
81	1490.0
82	1490.0
83	1213.5
84	937.0
85	715.0
86	493.0
87	368.0
88	243.0
89	243.0
90	185.5
91	128.0
92	78.5
93	29.0
94	17.5
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	804676.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.17206295914226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.2987992942631	37.678239327654296
2	6.626083578997474	5.853755620480302
3	2.2953798982752383	3.041749961452901
4	1.2170003644992904	2.1502966688784695
5	0.7593837027810018	1.677177236469449
6	0.553513023754806	1.4669887280401497
7	0.39296522482785623	1.215065925629469
8	0.3268009207136255	1.1548376675894338
9	0.24654425442439945	0.9801331495784424
>10	1.8470371100974436	16.281282652403796
>50	0.23370368099269317	7.2887365201706675
>100	0.18775703846009875	16.21000974164986
>500	0.012479319776801837	3.590434033696044
>1k	0.002552588136164012	1.4112927663067774
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1333	0.16565673637588296	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1330	0.1652839155138217	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1326	0.16478682103107337	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1320	0.16404117930695086	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1313	0.1631712639621413	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1263	0.15695758292778708	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1187	0.1475127877555687	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1105	0.1373223508592278	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1088	0.13520969930754737	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	995	0.12365225258364858	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	983	0.12216096913540357	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	980	0.12178814827334232	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	954	0.11855703413547813	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	889	0.11047924879081768	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	880	0.10936078620463392	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	876	0.10886369172188558	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.24273620687084E-4	0.0	0.0	0.0	0.0
3	1.24273620687084E-4	0.0	0.0	0.0	0.0
4	1.24273620687084E-4	0.0	0.0	0.0	0.0
5	1.24273620687084E-4	0.0	0.0	0.0	1.24273620687084E-4
6	1.24273620687084E-4	0.0	0.0	0.0	1.24273620687084E-4
7	1.24273620687084E-4	0.0	0.0	0.0	1.24273620687084E-4
8	2.48547241374168E-4	0.0	0.0	0.0	1.24273620687084E-4
9	3.7282086206125197E-4	0.0	0.0	0.0	1.24273620687084E-4
10	3.7282086206125197E-4	0.0	0.0	1.24273620687084E-4	1.24273620687084E-4
11	3.7282086206125197E-4	0.0	0.0	1.24273620687084E-4	2.48547241374168E-4
12	3.7282086206125197E-4	0.0	0.0	1.24273620687084E-4	2.48547241374168E-4
13	3.7282086206125197E-4	0.0	0.0	1.24273620687084E-4	2.48547241374168E-4
14	3.7282086206125197E-4	0.0	0.0	1.24273620687084E-4	2.48547241374168E-4
15	4.97094482748336E-4	0.0	0.0	1.24273620687084E-4	3.7282086206125197E-4
16	4.97094482748336E-4	0.0	0.0	4.97094482748336E-4	3.7282086206125197E-4
17	4.97094482748336E-4	0.0	0.0	7.456417241225039E-4	4.97094482748336E-4
18	7.456417241225039E-4	0.0	0.0	0.00124273620687084	4.97094482748336E-4
19	7.456417241225039E-4	0.0	0.0	0.00124273620687084	6.2136810343542E-4
20	7.456417241225039E-4	0.0	0.0	0.0014912834482450079	6.2136810343542E-4
21	7.456417241225039E-4	0.0	0.0	0.003355387758551268	7.456417241225039E-4
22	7.456417241225039E-4	0.0	0.0	0.006835049137789619	7.456417241225039E-4
23	7.456417241225039E-4	1.24273620687084E-4	0.0	0.008823427068782964	7.456417241225039E-4
24	7.456417241225039E-4	1.24273620687084E-4	0.0	0.010687531379089224	7.456417241225039E-4
25	7.456417241225039E-4	1.24273620687084E-4	0.0	0.0124273620687084	7.456417241225039E-4
26	7.456417241225039E-4	1.24273620687084E-4	0.0	0.016652665172069255	8.69915344809588E-4
27	7.456417241225039E-4	1.24273620687084E-4	0.0	0.03939473775780562	8.69915344809588E-4
28	7.456417241225039E-4	1.24273620687084E-4	0.0	0.14552440982457535	8.69915344809588E-4
29	7.456417241225039E-4	1.24273620687084E-4	0.0	0.3057131068902266	8.69915344809588E-4
30	7.456417241225039E-4	1.24273620687084E-4	0.0	0.5138714215410923	8.69915344809588E-4
31	7.456417241225039E-4	1.24273620687084E-4	0.0	1.1225636156664298	8.69915344809588E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1105	0.0	29.968325	1
ATACGGC	60	4.3193722E-8	27.750002	29
TTTTACG	55	1.9017387E-5	23.545456	4
CTAGACC	40	0.0019306868	23.125	3
AATACGC	40	0.0019306868	23.125	5
TTACGAG	60	3.7244972E-5	21.583334	6
GACAGGC	405	0.0	21.469135	7
GTACTAT	70	5.0987874E-6	21.142857	1
GCGGTAA	540	0.0	20.89815	23
TAGACCA	45	0.003824826	20.555555	4
ACCGCTC	45	0.003824826	20.555555	8
CTTATAC	925	0.0	20.0	37
GACCGTT	65	6.898845E-5	19.923077	7
CATCAGA	420	0.0	19.38095	16
GTATCAA	1720	0.0	19.252907	2
CGGTAAT	595	0.0	18.966387	24
GGACAGG	475	0.0	18.694736	6
CCTAGAC	60	9.233531E-4	18.5	2
GATTTAT	50	0.0070330915	18.5	1
GGACGTA	60	9.233531E-4	18.5	27
>>END_MODULE