FastQCFastQC Report
Fri 10 Feb 2017
ERR1631941.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631941.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308548
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7280.23594384018045816No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC6880.22297989291779563No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6480.2100159456551331No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6050.19607970234777083No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5400.17501328804594424No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT5190.1682072157330464No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4680.15167818297315167No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4420.14325161725242103No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4260.138066038347356No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4130.13385275548699066No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4080.13223226207915786No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4020.1302876699897585No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3960.1283430779003591No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3840.12445389372156034No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG3710.12024061086119502No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3420.11084174909576468No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3400.11019355173263155No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT3210.10403567678286686No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG3200.10371157810130029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGACC451.075623E-732.8888937
GTATTGT250.005490190329.5999981
AAATTTA250.005490190329.59999818
GGTATCA4150.028.530121
GTTCTAA358.8546355E-426.428571
GCGGTAA3250.023.3384623
ATTAGAG802.7093847E-823.1253
CGGTAAT3300.022.98484824
AGCCGCG3500.022.7285719
CACATGT753.7224345E-722.228
TGAGTAT753.7224345E-722.23
TAATACG3100.022.08064527
GGTAATA3700.022.025
CATCAGA2450.021.89795916
CGCGGTA3550.021.88732322
GACACAT855.149741E-821.76470626
AGAAGAG2550.021.76470620
AGTGACA855.149741E-821.76470623
TATTAGA855.149741E-821.7647062
TTTAACG603.714922E-521.58333434
Produced by FastQC (version 0.11.5)